Lewinella sp. 4G2

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Saprospiria; Saprospirales; Lewinellaceae; Lewinella; unclassified Lewinella

Average proteome isoelectric point is 5.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3730 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A180ESP5|A0A180ESP5_9BACT 50S ribosomal protein L18 OS=Lewinella sp. 4G2 OX=1803372 GN=rplR PE=3 SV=1
MM1 pKa = 7.5TFFKK5 pKa = 10.84KK6 pKa = 10.44SITLICLVLLASTLSGQSFSDD27 pKa = 4.21DD28 pKa = 3.34FAADD32 pKa = 4.15LSNWPVTAGWQWDD45 pKa = 3.7ADD47 pKa = 3.74GKK49 pKa = 11.04ADD51 pKa = 5.58NIPNVNWNNRR61 pKa = 11.84SRR63 pKa = 11.84LRR65 pKa = 11.84SASEE69 pKa = 3.95GGAALYY75 pKa = 10.47DD76 pKa = 3.79SSTGAGRR83 pKa = 11.84LTSRR87 pKa = 11.84IFTPDD92 pKa = 3.34TEE94 pKa = 4.86PGLFVSFYY102 pKa = 10.72HH103 pKa = 6.23YY104 pKa = 10.23FQNQGGQIRR113 pKa = 11.84LQVIEE118 pKa = 4.14SGGNVLYY125 pKa = 10.56DD126 pKa = 3.7EE127 pKa = 4.88PLVPNVVSDD136 pKa = 4.11EE137 pKa = 4.14EE138 pKa = 4.38TSAGNFQIIDD148 pKa = 3.69LSFLKK153 pKa = 10.37TNGLNDD159 pKa = 3.51FQISFEE165 pKa = 4.09VATNIEE171 pKa = 4.39FWLLDD176 pKa = 3.86DD177 pKa = 4.1VTVGTDD183 pKa = 3.18RR184 pKa = 11.84PAWPTFPIYY193 pKa = 10.62FGQQLTNFGIPFNVDD208 pKa = 2.87SLAMPFVPFEE218 pKa = 4.19VVLDD222 pKa = 4.83FAATATPAIRR232 pKa = 11.84DD233 pKa = 4.0AIRR236 pKa = 11.84AEE238 pKa = 3.8FDD240 pKa = 3.17IVSVEE245 pKa = 4.0SCVCDD250 pKa = 3.63RR251 pKa = 11.84LEE253 pKa = 3.71VWTMGGGVFFDD264 pKa = 4.21EE265 pKa = 5.1ATGQPLGPPGDD276 pKa = 3.37ILEE279 pKa = 4.49RR280 pKa = 11.84VLGSSTNSGAEE291 pKa = 3.79SGEE294 pKa = 3.77VDD296 pKa = 3.84NIEE299 pKa = 4.84LNVLTYY305 pKa = 10.99DD306 pKa = 3.98EE307 pKa = 5.42LLSNPNAPTSPLQSGDD323 pKa = 3.53LAFLTDD329 pKa = 3.96APAGAVKK336 pKa = 10.5VAVLDD341 pKa = 3.61TGLDD345 pKa = 3.58LDD347 pKa = 4.8HH348 pKa = 7.31PTLQPYY354 pKa = 10.09LYY356 pKa = 10.87KK357 pKa = 10.62NDD359 pKa = 4.36DD360 pKa = 3.84PLGDD364 pKa = 4.78GEE366 pKa = 6.07DD367 pKa = 4.63DD368 pKa = 5.41DD369 pKa = 6.38SNCQTDD375 pKa = 6.12DD376 pKa = 4.38LIGWNFVDD384 pKa = 5.45GNNNPDD390 pKa = 4.12DD391 pKa = 3.96GHH393 pKa = 7.07GHH395 pKa = 5.23GTHH398 pKa = 5.77VAGIVAQTVSEE409 pKa = 4.32CDD411 pKa = 3.09NCTVQIMPYY420 pKa = 8.33KK421 pKa = 8.54THH423 pKa = 7.6DD424 pKa = 3.51NFGVGTVFNAACAVLQASVYY444 pKa = 11.05DD445 pKa = 3.84EE446 pKa = 4.86ADD448 pKa = 3.51VINASWGFYY457 pKa = 10.18GQGSDD462 pKa = 2.76ILRR465 pKa = 11.84RR466 pKa = 11.84AIDD469 pKa = 3.26TAAVYY474 pKa = 9.8GALVVAAVGNDD485 pKa = 3.74SLNMVADD492 pKa = 4.08DD493 pKa = 4.54QFPATYY499 pKa = 9.68TSNTVMSVAAFDD511 pKa = 3.99FDD513 pKa = 4.34GVSEE517 pKa = 4.74LSLAPYY523 pKa = 10.64SNYY526 pKa = 9.97NPSFADD532 pKa = 2.9ISYY535 pKa = 10.29YY536 pKa = 9.49GTAINSALPGGGLGNKK552 pKa = 8.6TGTSMATPALSATAALHH569 pKa = 6.61LCMTPSLSAAALRR582 pKa = 11.84TEE584 pKa = 4.35ILNNAYY590 pKa = 10.37DD591 pKa = 3.68NSAEE595 pKa = 3.99LGTFIFNGRR604 pKa = 11.84IPDD607 pKa = 3.75IEE609 pKa = 4.81DD610 pKa = 3.29VCEE613 pKa = 4.02MTDD616 pKa = 3.34PGNDD620 pKa = 3.28GTAAPYY626 pKa = 9.45SACICDD632 pKa = 4.35DD633 pKa = 3.76EE634 pKa = 4.76QPSVVSVTADD644 pKa = 3.37SPEE647 pKa = 3.9GEE649 pKa = 4.08YY650 pKa = 10.27TIRR653 pKa = 11.84VLTLSGEE660 pKa = 4.14EE661 pKa = 4.09LNRR664 pKa = 11.84VDD666 pKa = 4.98NAPLEE671 pKa = 4.54AGQPLNVEE679 pKa = 4.8LGTDD683 pKa = 3.48EE684 pKa = 5.01PGTYY688 pKa = 10.38LIVIKK693 pKa = 10.73NDD695 pKa = 2.82LEE697 pKa = 4.38TRR699 pKa = 11.84VLRR702 pKa = 11.84VVRR705 pKa = 11.84QDD707 pKa = 2.85

Molecular weight: 75.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A180EMX3|A0A180EMX3_9BACT Uncharacterized protein OS=Lewinella sp. 4G2 OX=1803372 GN=A3850_015780 PE=4 SV=1
MM1 pKa = 7.24GSRR4 pKa = 11.84YY5 pKa = 10.46AFVGAAAGARR15 pKa = 11.84QWASKK20 pKa = 10.53RR21 pKa = 11.84CVTTALKK28 pKa = 10.88YY29 pKa = 10.41ILFRR33 pKa = 11.84NNRR36 pKa = 11.84AKK38 pKa = 11.18GNDD41 pKa = 3.38CQINLTEE48 pKa = 4.61RR49 pKa = 11.84GAFVDD54 pKa = 4.09RR55 pKa = 11.84LSKK58 pKa = 10.97ADD60 pKa = 3.28ARR62 pKa = 11.84LNNTRR67 pKa = 11.84FPQLVGLTAAPLALVSSLAVSDD89 pKa = 4.77KK90 pKa = 10.95II91 pKa = 4.64

Molecular weight: 9.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3730

0

3730

1497538

38

16096

401.5

44.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.817 ± 0.046

0.887 ± 0.028

6.463 ± 0.092

6.234 ± 0.038

4.464 ± 0.034

8.079 ± 0.065

1.696 ± 0.039

5.487 ± 0.031

3.917 ± 0.08

9.682 ± 0.09

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.994 ± 0.027

4.458 ± 0.041

4.57 ± 0.035

3.548 ± 0.025

5.527 ± 0.077

5.822 ± 0.032

6.591 ± 0.122

6.932 ± 0.053

1.202 ± 0.021

3.63 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski