Cryobacterium arcticum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Cryobacterium

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3936 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B1BNE9|A0A1B1BNE9_9MICO Transcriptional regulator OS=Cryobacterium arcticum OX=670052 GN=PA27867_3263 PE=3 SV=1
MM1 pKa = 6.82ATKK4 pKa = 10.42RR5 pKa = 11.84HH6 pKa = 6.14PATFLGAGVAATVLFLSGCAANEE29 pKa = 4.27AGDD32 pKa = 4.31EE33 pKa = 4.15PSDD36 pKa = 4.54LAGTLDD42 pKa = 3.8GAGSSAQASAEE53 pKa = 4.2DD54 pKa = 3.5VWISQFQRR62 pKa = 11.84ANGSVTINYY71 pKa = 8.96DD72 pKa = 3.27PTGSGAGRR80 pKa = 11.84EE81 pKa = 3.99QFIGGGVNFAGSDD94 pKa = 3.48SALSDD99 pKa = 4.09DD100 pKa = 4.32EE101 pKa = 4.61LAGSFGACAPGSAAIDD117 pKa = 3.62LPVYY121 pKa = 10.27VSPLVLVYY129 pKa = 10.82NVDD132 pKa = 3.66GVDD135 pKa = 3.89SLRR138 pKa = 11.84LDD140 pKa = 3.79PQTIASIYY148 pKa = 10.16SGAITRR154 pKa = 11.84WNDD157 pKa = 3.13PALVALNPDD166 pKa = 3.31AGLPDD171 pKa = 3.77EE172 pKa = 5.66PISAVHH178 pKa = 6.78RR179 pKa = 11.84SDD181 pKa = 5.84DD182 pKa = 3.99SGTTKK187 pKa = 10.73NFADD191 pKa = 3.8YY192 pKa = 10.6LYY194 pKa = 11.1QNVPDD199 pKa = 3.39VWTNEE204 pKa = 3.91PADD207 pKa = 3.77AFPYY211 pKa = 9.94PGEE214 pKa = 4.25GAQGNSGVVSAVANGRR230 pKa = 11.84NTIGYY235 pKa = 9.71ADD237 pKa = 4.09ASRR240 pKa = 11.84VGDD243 pKa = 3.86LSVAALKK250 pKa = 10.68VGNDD254 pKa = 3.32FVDD257 pKa = 3.94YY258 pKa = 9.63STEE261 pKa = 3.54AAAAIIDD268 pKa = 4.1EE269 pKa = 4.67SPLVARR275 pKa = 11.84DD276 pKa = 3.69NPNDD280 pKa = 3.6LVVDD284 pKa = 3.73VDD286 pKa = 4.06RR287 pKa = 11.84TSTAAGVYY295 pKa = 9.4PLVLVSYY302 pKa = 10.12LIACEE307 pKa = 4.35EE308 pKa = 4.27YY309 pKa = 10.26PDD311 pKa = 4.51ADD313 pKa = 3.53QAEE316 pKa = 4.77LVSAYY321 pKa = 10.0LGYY324 pKa = 10.25VASPEE329 pKa = 4.25GQNAAAEE336 pKa = 4.57SAGSAPLSAEE346 pKa = 4.07FSARR350 pKa = 11.84VLDD353 pKa = 5.24AIDD356 pKa = 4.07SIRR359 pKa = 3.71

Molecular weight: 36.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B1BQL1|A0A1B1BQL1_9MICO Uncharacterized protein OS=Cryobacterium arcticum OX=670052 GN=PA27867_3937 PE=4 SV=1
MM1 pKa = 7.44SANSLKK7 pKa = 10.63RR8 pKa = 11.84KK9 pKa = 9.57LLLIKK14 pKa = 9.94QLHH17 pKa = 5.92KK18 pKa = 10.71RR19 pKa = 11.84GPTDD23 pKa = 3.22PKK25 pKa = 10.24EE26 pKa = 3.82IRR28 pKa = 11.84RR29 pKa = 11.84LLSCEE34 pKa = 3.85RR35 pKa = 11.84LRR37 pKa = 11.84LRR39 pKa = 11.84HH40 pKa = 4.86NTHH43 pKa = 7.07RR44 pKa = 11.84KK45 pKa = 8.45PPLHH49 pKa = 5.96GVNYY53 pKa = 9.86LQEE56 pKa = 5.1HH57 pKa = 6.49PMNLFWDD64 pKa = 4.19LDD66 pKa = 3.86LVTRR70 pKa = 11.84CGAGEE75 pKa = 4.21KK76 pKa = 8.18VTRR79 pKa = 11.84DD80 pKa = 3.21RR81 pKa = 11.84GLSTGSLVRR90 pKa = 11.84RR91 pKa = 11.84EE92 pKa = 4.52KK93 pKa = 10.28IVHH96 pKa = 6.68PGDD99 pKa = 3.33TSQVVAYY106 pKa = 10.11VCLLAQCHH114 pKa = 5.12RR115 pKa = 11.84VAHH118 pKa = 6.17NYY120 pKa = 10.74SLFARR125 pKa = 11.84NVTHH129 pKa = 7.41LRR131 pKa = 11.84IFF133 pKa = 3.73

Molecular weight: 15.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3936

0

3936

1284498

50

2228

326.3

34.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.351 ± 0.055

0.508 ± 0.009

5.989 ± 0.037

5.045 ± 0.041

3.184 ± 0.023

8.885 ± 0.035

1.924 ± 0.021

4.58 ± 0.028

2.035 ± 0.028

10.531 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.766 ± 0.016

2.238 ± 0.021

5.516 ± 0.028

2.883 ± 0.021

6.627 ± 0.046

5.952 ± 0.03

6.685 ± 0.039

8.796 ± 0.037

1.469 ± 0.02

2.038 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski