Deinococcus hopiensis KR-140

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Deinococcus-Thermus; Deinococci; Deinococcales; Deinococcaceae; Deinococcus; Deinococcus hopiensis

Average proteome isoelectric point is 7.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5843 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W1UCL2|A0A1W1UCL2_9DEIO Dihydroorotate dehydrogenase OS=Deinococcus hopiensis KR-140 OX=695939 GN=SAMN00790413_05652 PE=4 SV=1
MM1 pKa = 7.46PHH3 pKa = 6.98LKK5 pKa = 10.65ALMLSALIATTALFTSASATPVYY28 pKa = 10.25FSNDD32 pKa = 3.12SGQSGVVDD40 pKa = 3.97VYY42 pKa = 11.93VDD44 pKa = 3.57GQLVFDD50 pKa = 4.87NVFADD55 pKa = 4.03DD56 pKa = 4.27SMMFPRR62 pKa = 11.84EE63 pKa = 3.89LSAGQHH69 pKa = 4.87NVVVTPNYY77 pKa = 10.38LPLGEE82 pKa = 4.05QDD84 pKa = 3.34ILRR87 pKa = 11.84TTLTVPEE94 pKa = 4.21EE95 pKa = 4.34TAGNTAYY102 pKa = 9.69TINLATEE109 pKa = 4.04TDD111 pKa = 3.7DD112 pKa = 5.65LGVEE116 pKa = 4.36GLTLTWNTSQIDD128 pKa = 3.37

Molecular weight: 13.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W1VF05|A0A1W1VF05_9DEIO Uncharacterized protein OS=Deinococcus hopiensis KR-140 OX=695939 GN=SAMN00790413_01405 PE=4 SV=1
MM1 pKa = 7.86PPNLNVSRR9 pKa = 11.84AAPGAPFFRR18 pKa = 11.84YY19 pKa = 8.93SVAMKK24 pKa = 10.2RR25 pKa = 11.84LWFALFAASLLGACGTVATPASSTPRR51 pKa = 11.84TAGEE55 pKa = 3.98LLGAPTSLQVSGRR68 pKa = 11.84SVRR71 pKa = 11.84ADD73 pKa = 3.48AAPVLAGDD81 pKa = 4.31TFRR84 pKa = 11.84VRR86 pKa = 11.84VKK88 pKa = 10.28VQASRR93 pKa = 11.84APLPPLTVTGVYY105 pKa = 10.02VVTEE109 pKa = 4.28DD110 pKa = 3.95GVWRR114 pKa = 11.84AGRR117 pKa = 11.84TRR119 pKa = 11.84SGEE122 pKa = 4.11AGCAAQSCVQGTGEE136 pKa = 4.17GAASGLRR143 pKa = 11.84AGDD146 pKa = 3.65GVQVVVSLKK155 pKa = 10.53DD156 pKa = 3.22AGGRR160 pKa = 11.84TMWLRR165 pKa = 11.84DD166 pKa = 3.45PQASIKK172 pKa = 10.6

Molecular weight: 17.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5843

0

5843

1675551

29

3868

286.8

31.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.024 ± 0.049

0.676 ± 0.009

4.755 ± 0.022

5.734 ± 0.034

3.157 ± 0.02

9.054 ± 0.033

2.293 ± 0.02

3.248 ± 0.028

2.568 ± 0.027

11.656 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.842 ± 0.014

2.479 ± 0.022

5.89 ± 0.029

3.958 ± 0.028

7.682 ± 0.035

5.225 ± 0.026

6.163 ± 0.04

7.86 ± 0.028

1.451 ± 0.015

2.284 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski