Amycolatopsis saalfeldensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Amycolatopsis

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9167 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H8YIQ7|A0A1H8YIQ7_9PSEU Arabinofuranosyltransferase OS=Amycolatopsis saalfeldensis OX=394193 GN=SAMN04489732_118130 PE=4 SV=1
MM1 pKa = 7.38WVDD4 pKa = 3.3EE5 pKa = 4.53SGGTDD10 pKa = 3.19TSGTDD15 pKa = 2.89AMTVHH20 pKa = 6.71VDD22 pKa = 3.3GQEE25 pKa = 3.95YY26 pKa = 8.88QAEE29 pKa = 4.44VNFDD33 pKa = 3.63SDD35 pKa = 3.56HH36 pKa = 7.32DD37 pKa = 4.45GVSDD41 pKa = 3.68TAIVEE46 pKa = 4.22HH47 pKa = 7.7DD48 pKa = 4.7DD49 pKa = 3.88GSAQAFTDD57 pKa = 3.51TDD59 pKa = 3.67HH60 pKa = 7.81DD61 pKa = 4.12GTADD65 pKa = 3.38HH66 pKa = 6.69YY67 pKa = 11.71AVLDD71 pKa = 3.91STGHH75 pKa = 5.46VVDD78 pKa = 3.56QAVYY82 pKa = 10.88DD83 pKa = 4.13EE84 pKa = 5.81ASGAWVEE91 pKa = 4.46SGGGHH96 pKa = 7.17PDD98 pKa = 2.78SHH100 pKa = 7.75SQDD103 pKa = 3.87DD104 pKa = 4.03STTGHH109 pKa = 6.12IHH111 pKa = 7.0ADD113 pKa = 3.6LPGGEE118 pKa = 4.01VDD120 pKa = 5.07AGVATIDD127 pKa = 3.96TNHH130 pKa = 7.3DD131 pKa = 3.42GHH133 pKa = 6.97NDD135 pKa = 3.36TAIVDD140 pKa = 4.13TKK142 pKa = 11.2SGGTIAFTDD151 pKa = 3.65TNGDD155 pKa = 3.58GEE157 pKa = 4.51ADD159 pKa = 3.11IAVQIDD165 pKa = 3.61ADD167 pKa = 4.09GTTTTFDD174 pKa = 3.21HH175 pKa = 6.64TGAGQWTEE183 pKa = 3.76EE184 pKa = 4.16SGGNGAAAPADD195 pKa = 3.68PAGDD199 pKa = 4.17HH200 pKa = 6.36LWGGDD205 pKa = 3.33GTQLLNGVAKK215 pKa = 9.89IDD217 pKa = 3.73SGTGQWISPNN227 pKa = 3.39

Molecular weight: 23.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H8YNJ0|A0A1H8YNJ0_9PSEU 3'-phosphate/5'-hydroxy nucleic acid ligase OS=Amycolatopsis saalfeldensis OX=394193 GN=SAMN04489732_12364 PE=3 SV=1
MM1 pKa = 7.55TSQAHH6 pKa = 4.8NRR8 pKa = 11.84RR9 pKa = 11.84GNRR12 pKa = 11.84QRR14 pKa = 11.84AGFPAGWRR22 pKa = 11.84RR23 pKa = 11.84SRR25 pKa = 11.84RR26 pKa = 11.84CRR28 pKa = 11.84RR29 pKa = 11.84CRR31 pKa = 11.84RR32 pKa = 11.84GISLRR37 pKa = 11.84RR38 pKa = 11.84TRR40 pKa = 11.84SRR42 pKa = 11.84SRR44 pKa = 11.84GLGRR48 pKa = 11.84AGSSRR53 pKa = 11.84RR54 pKa = 11.84PGARR58 pKa = 11.84RR59 pKa = 11.84VRR61 pKa = 11.84RR62 pKa = 11.84SRR64 pKa = 11.84GSGRR68 pKa = 11.84GGSNRR73 pKa = 11.84HH74 pKa = 5.02LGARR78 pKa = 11.84RR79 pKa = 11.84VRR81 pKa = 11.84RR82 pKa = 11.84SRR84 pKa = 11.84RR85 pKa = 11.84VRR87 pKa = 11.84RR88 pKa = 11.84VGRR91 pKa = 11.84RR92 pKa = 11.84RR93 pKa = 11.84RR94 pKa = 11.84GVRR97 pKa = 11.84VRR99 pKa = 11.84SRR101 pKa = 11.84RR102 pKa = 11.84RR103 pKa = 11.84RR104 pKa = 11.84EE105 pKa = 3.78SVRR108 pKa = 11.84AGSRR112 pKa = 11.84RR113 pKa = 11.84RR114 pKa = 11.84PGVRR118 pKa = 11.84RR119 pKa = 11.84VGSSRR124 pKa = 11.84RR125 pKa = 11.84KK126 pKa = 9.02LGRR129 pKa = 11.84VGSRR133 pKa = 11.84RR134 pKa = 11.84RR135 pKa = 11.84RR136 pKa = 11.84GAGRR140 pKa = 11.84VGSRR144 pKa = 11.84HH145 pKa = 5.99RR146 pKa = 11.84GLGRR150 pKa = 11.84VGSRR154 pKa = 11.84HH155 pKa = 5.49RR156 pKa = 11.84PGVGLVRR163 pKa = 11.84RR164 pKa = 11.84RR165 pKa = 11.84RR166 pKa = 11.84RR167 pKa = 11.84GLVGSRR173 pKa = 11.84RR174 pKa = 11.84RR175 pKa = 11.84PGVRR179 pKa = 11.84RR180 pKa = 11.84VGSRR184 pKa = 11.84RR185 pKa = 11.84VRR187 pKa = 11.84RR188 pKa = 11.84GGSSSRR194 pKa = 11.84GRR196 pKa = 11.84VVLRR200 pKa = 11.84GGRR203 pKa = 11.84NSRR206 pKa = 11.84GRR208 pKa = 11.84EE209 pKa = 3.88GGRR212 pKa = 11.84SRR214 pKa = 11.84GVCRR218 pKa = 11.84VGGRR222 pKa = 11.84RR223 pKa = 11.84SRR225 pKa = 11.84RR226 pKa = 11.84RR227 pKa = 11.84GSGLGG232 pKa = 3.34

Molecular weight: 26.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9167

0

9167

2936693

11

9301

320.4

34.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.651 ± 0.038

0.726 ± 0.008

5.842 ± 0.019

5.437 ± 0.027

2.919 ± 0.013

9.561 ± 0.027

2.184 ± 0.012

3.288 ± 0.02

2.115 ± 0.018

10.509 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.618 ± 0.01

1.89 ± 0.014

6.064 ± 0.024

2.88 ± 0.017

7.646 ± 0.027

5.33 ± 0.023

6.052 ± 0.02

8.747 ± 0.024

1.537 ± 0.012

2.002 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski