Roseovarius azorensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Roseovarius

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3792 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H7V2W4|A0A1H7V2W4_9RHOB Putative thiamine transport system ATP-binding protein OS=Roseovarius azorensis OX=1287727 GN=SAMN05443999_111109 PE=4 SV=1
MM1 pKa = 7.59LKK3 pKa = 7.82TTTALIAATAFFAAPALAGNISEE26 pKa = 4.61PAPEE30 pKa = 4.17PVIATPVAPPAPATPNWTGFYY51 pKa = 10.54AGGQLGYY58 pKa = 10.75GWVDD62 pKa = 3.0TDD64 pKa = 3.84TPGVDD69 pKa = 3.55GDD71 pKa = 4.81DD72 pKa = 4.1IIGGLILGYY81 pKa = 10.68DD82 pKa = 3.76YY83 pKa = 11.59DD84 pKa = 5.25LGNNWVIGAGIDD96 pKa = 3.99YY97 pKa = 10.9DD98 pKa = 3.85FADD101 pKa = 4.28INLSGAGSLEE111 pKa = 3.86EE112 pKa = 4.0VFRR115 pKa = 11.84AKK117 pKa = 10.65LRR119 pKa = 11.84GGYY122 pKa = 10.07KK123 pKa = 9.6IGNGLLYY130 pKa = 9.66GTGGYY135 pKa = 10.01AWADD139 pKa = 3.38TDD141 pKa = 4.8VLGDD145 pKa = 3.53DD146 pKa = 3.22SGYY149 pKa = 10.61FIGGGYY155 pKa = 8.58EE156 pKa = 4.28HH157 pKa = 7.24MVTQNISLGGEE168 pKa = 4.05LLYY171 pKa = 10.91HH172 pKa = 5.99QFDD175 pKa = 4.13NFGSTGADD183 pKa = 2.69VDD185 pKa = 3.86ATTLQARR192 pKa = 11.84AAFRR196 pKa = 11.84FF197 pKa = 3.75

Molecular weight: 20.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H7UDH4|A0A1H7UDH4_9RHOB Shikimate dehydrogenase OS=Roseovarius azorensis OX=1287727 GN=SAMN05443999_11047 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 8.83HH14 pKa = 4.64RR15 pKa = 11.84HH16 pKa = 3.91GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.43VLCAA44 pKa = 3.88

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3792

0

3792

1160880

25

1847

306.1

33.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.43 ± 0.054

0.952 ± 0.014

5.864 ± 0.032

5.817 ± 0.038

3.62 ± 0.022

8.79 ± 0.041

2.162 ± 0.021

5.239 ± 0.032

2.703 ± 0.027

10.171 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.869 ± 0.018

2.478 ± 0.02

5.136 ± 0.026

3.097 ± 0.019

7.527 ± 0.041

4.832 ± 0.024

5.376 ± 0.024

7.279 ± 0.035

1.436 ± 0.017

2.222 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski