Amino acid dipepetide frequency for Thermodesulfobacterium geofontis (strain OPF15)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.14AlaAla: 2.14 ± 0.085
0.829AlaCys: 0.829 ± 0.052
1.976AlaAsp: 1.976 ± 0.075
4.322AlaGlu: 4.322 ± 0.103
2.9AlaPhe: 2.9 ± 0.084
3.874AlaGly: 3.874 ± 0.112
0.979AlaHis: 0.979 ± 0.045
5.26AlaIle: 5.26 ± 0.121
5.657AlaLys: 5.657 ± 0.122
7.321AlaLeu: 7.321 ± 0.121
1.027AlaMet: 1.027 ± 0.055
1.82AlaAsn: 1.82 ± 0.063
1.688AlaPro: 1.688 ± 0.057
1.696AlaGln: 1.696 ± 0.058
2.073AlaArg: 2.073 ± 0.071
2.677AlaSer: 2.677 ± 0.084
2.496AlaThr: 2.496 ± 0.094
3.137AlaVal: 3.137 ± 0.082
0.48AlaTrp: 0.48 ± 0.031
2.49AlaTyr: 2.49 ± 0.071
0.0AlaXaa: 0.0 ± 0.0
Cys
0.634CysAla: 0.634 ± 0.039
0.126CysCys: 0.126 ± 0.016
0.385CysAsp: 0.385 ± 0.028
0.851CysGlu: 0.851 ± 0.041
0.555CysPhe: 0.555 ± 0.031
1.033CysGly: 1.033 ± 0.053
0.316CysHis: 0.316 ± 0.072
0.746CysIle: 0.746 ± 0.035
0.932CysLys: 0.932 ± 0.059
0.875CysLeu: 0.875 ± 0.047
0.154CysMet: 0.154 ± 0.018
0.344CysAsn: 0.344 ± 0.031
0.727CysPro: 0.727 ± 0.047
0.251CysGln: 0.251 ± 0.023
0.324CysArg: 0.324 ± 0.025
0.6CysSer: 0.6 ± 0.035
0.353CysThr: 0.353 ± 0.032
0.533CysVal: 0.533 ± 0.036
0.103CysTrp: 0.103 ± 0.014
0.409CysTyr: 0.409 ± 0.029
0.0CysXaa: 0.0 ± 0.0
Asp
1.876AspAla: 1.876 ± 0.073
0.413AspCys: 0.413 ± 0.033
1.281AspAsp: 1.281 ± 0.053
3.426AspGlu: 3.426 ± 0.094
3.167AspPhe: 3.167 ± 0.079
2.184AspGly: 2.184 ± 0.072
0.551AspHis: 0.551 ± 0.031
4.061AspIle: 4.061 ± 0.089
3.69AspLys: 3.69 ± 0.082
5.998AspLeu: 5.998 ± 0.106
0.628AspMet: 0.628 ± 0.04
1.216AspAsn: 1.216 ± 0.047
2.413AspPro: 2.413 ± 0.086
0.74AspGln: 0.74 ± 0.043
1.449AspArg: 1.449 ± 0.062
1.69AspSer: 1.69 ± 0.055
1.579AspThr: 1.579 ± 0.061
2.231AspVal: 2.231 ± 0.075
0.622AspTrp: 0.622 ± 0.034
2.059AspTyr: 2.059 ± 0.071
0.0AspXaa: 0.0 ± 0.0
Glu
4.598GluAla: 4.598 ± 0.1
0.533GluCys: 0.533 ± 0.036
4.028GluAsp: 4.028 ± 0.104
10.044GluGlu: 10.044 ± 0.184
3.83GluPhe: 3.83 ± 0.089
4.95GluGly: 4.95 ± 0.123
0.837GluHis: 0.837 ± 0.042
10.423GluIle: 10.423 ± 0.164
12.095GluLys: 12.095 ± 0.2
8.342GluLeu: 8.342 ± 0.165
1.56GluMet: 1.56 ± 0.065
4.942GluAsn: 4.942 ± 0.11
2.269GluPro: 2.269 ± 0.069
1.046GluGln: 1.046 ± 0.054
4.158GluArg: 4.158 ± 0.113
2.861GluSer: 2.861 ± 0.077
3.666GluThr: 3.666 ± 0.082
5.974GluVal: 5.974 ± 0.109
0.638GluTrp: 0.638 ± 0.044
2.675GluTyr: 2.675 ± 0.067
0.0GluXaa: 0.0 ± 0.0
Phe
2.454PheAla: 2.454 ± 0.083
0.709PheCys: 0.709 ± 0.039
2.298PheAsp: 2.298 ± 0.075
4.045PheGlu: 4.045 ± 0.105
3.333PhePhe: 3.333 ± 0.119
3.145PheGly: 3.145 ± 0.083
0.819PheHis: 0.819 ± 0.046
5.236PheIle: 5.236 ± 0.129
5.313PheLys: 5.313 ± 0.102
7.747PheLeu: 7.747 ± 0.194
0.938PheMet: 0.938 ± 0.046
2.231PheAsn: 2.231 ± 0.062
2.346PhePro: 2.346 ± 0.065
1.37PheGln: 1.37 ± 0.051
1.85PheArg: 1.85 ± 0.065
4.166PheSer: 4.166 ± 0.101
2.312PheThr: 2.312 ± 0.091
2.758PheVal: 2.758 ± 0.087
0.699PheTrp: 0.699 ± 0.042
2.869PheTyr: 2.869 ± 0.087
0.0PheXaa: 0.0 ± 0.0
Gly
3.856GlyAla: 3.856 ± 0.111
0.738GlyCys: 0.738 ± 0.049
2.598GlyAsp: 2.598 ± 0.087
4.74GlyGlu: 4.74 ± 0.098
3.625GlyPhe: 3.625 ± 0.088
4.111GlyGly: 4.111 ± 0.119
0.987GlyHis: 0.987 ± 0.049
6.427GlyIle: 6.427 ± 0.116
6.03GlyLys: 6.03 ± 0.112
6.095GlyLeu: 6.095 ± 0.108
1.108GlyMet: 1.108 ± 0.055
2.259GlyAsn: 2.259 ± 0.127
1.816GlyPro: 1.816 ± 0.059
1.11GlyGln: 1.11 ± 0.048
2.393GlyArg: 2.393 ± 0.083
2.924GlySer: 2.924 ± 0.089
2.916GlyThr: 2.916 ± 0.09
4.049GlyVal: 4.049 ± 0.106
0.681GlyTrp: 0.681 ± 0.04
2.764GlyTyr: 2.764 ± 0.072
0.0GlyXaa: 0.0 ± 0.0
His
0.677HisAla: 0.677 ± 0.037
0.178HisCys: 0.178 ± 0.021
0.432HisAsp: 0.432 ± 0.031
0.725HisGlu: 0.725 ± 0.037
0.867HisPhe: 0.867 ± 0.042
0.837HisGly: 0.837 ± 0.047
0.355HisHis: 0.355 ± 0.027
1.268HisIle: 1.268 ± 0.055
1.189HisLys: 1.189 ± 0.058
1.718HisLeu: 1.718 ± 0.062
0.249HisMet: 0.249 ± 0.022
0.547HisAsn: 0.547 ± 0.034
1.094HisPro: 1.094 ± 0.052
0.428HisGln: 0.428 ± 0.032
0.521HisArg: 0.521 ± 0.032
0.689HisSer: 0.689 ± 0.039
0.691HisThr: 0.691 ± 0.036
0.663HisVal: 0.663 ± 0.039
0.148HisTrp: 0.148 ± 0.017
0.636HisTyr: 0.636 ± 0.037
0.0HisXaa: 0.0 ± 0.0
Ile
5.481IleAla: 5.481 ± 0.122
0.932IleCys: 0.932 ± 0.048
4.251IleAsp: 4.251 ± 0.099
7.972IleGlu: 7.972 ± 0.147
5.597IlePhe: 5.597 ± 0.134
5.471IleGly: 5.471 ± 0.128
1.191IleHis: 1.191 ± 0.047
8.225IleIle: 8.225 ± 0.159
9.951IleLys: 9.951 ± 0.157
10.979IleLeu: 10.979 ± 0.166
1.4IleMet: 1.4 ± 0.054
4.075IleAsn: 4.075 ± 0.1
4.661IlePro: 4.661 ± 0.105
1.797IleGln: 1.797 ± 0.065
3.394IleArg: 3.394 ± 0.087
6.079IleSer: 6.079 ± 0.114
4.087IleThr: 4.087 ± 0.098
5.088IleVal: 5.088 ± 0.113
0.776IleTrp: 0.776 ± 0.039
3.84IleTyr: 3.84 ± 0.085
0.0IleXaa: 0.0 ± 0.0
Lys
5.901LysAla: 5.901 ± 0.125
0.709LysCys: 0.709 ± 0.05
5.279LysAsp: 5.279 ± 0.11
12.825LysGlu: 12.825 ± 0.234
4.217LysPhe: 4.217 ± 0.088
6.02LysGly: 6.02 ± 0.118
1.009LysHis: 1.009 ± 0.051
11.216LysIle: 11.216 ± 0.181
10.563LysLys: 10.563 ± 0.186
9.102LysLeu: 9.102 ± 0.147
1.686LysMet: 1.686 ± 0.054
5.797LysAsn: 5.797 ± 0.14
3.461LysPro: 3.461 ± 0.08
1.467LysGln: 1.467 ± 0.06
4.288LysArg: 4.288 ± 0.106
4.038LysSer: 4.038 ± 0.096
4.344LysThr: 4.344 ± 0.11
6.472LysVal: 6.472 ± 0.125
0.794LysTrp: 0.794 ± 0.042
3.615LysTyr: 3.615 ± 0.081
0.0LysXaa: 0.0 ± 0.0
Leu
6.646LeuAla: 6.646 ± 0.119
1.056LeuCys: 1.056 ± 0.046
4.375LeuAsp: 4.375 ± 0.1
10.241LeuGlu: 10.241 ± 0.196
5.651LeuPhe: 5.651 ± 0.141
6.851LeuGly: 6.851 ± 0.123
1.26LeuHis: 1.26 ± 0.051
10.014LeuIle: 10.014 ± 0.171
13.392LeuLys: 13.392 ± 0.235
10.5LeuLeu: 10.5 ± 0.217
1.759LeuMet: 1.759 ± 0.065
5.108LeuAsn: 5.108 ± 0.112
4.699LeuPro: 4.699 ± 0.113
2.436LeuGln: 2.436 ± 0.064
4.391LeuArg: 4.391 ± 0.111
7.368LeuSer: 7.368 ± 0.131
4.679LeuThr: 4.679 ± 0.093
5.736LeuVal: 5.736 ± 0.126
1.019LeuTrp: 1.019 ± 0.048
4.004LeuTyr: 4.004 ± 0.103
0.0LeuXaa: 0.0 ± 0.0
Met
1.193MetAla: 1.193 ± 0.054
0.156MetCys: 0.156 ± 0.019
0.756MetAsp: 0.756 ± 0.04
1.42MetGlu: 1.42 ± 0.059
0.659MetPhe: 0.659 ± 0.04
1.305MetGly: 1.305 ± 0.044
0.223MetHis: 0.223 ± 0.023
1.238MetIle: 1.238 ± 0.051
1.921MetLys: 1.921 ± 0.068
1.453MetLeu: 1.453 ± 0.055
0.251MetMet: 0.251 ± 0.022
0.65MetAsn: 0.65 ± 0.038
0.725MetPro: 0.725 ± 0.04
0.397MetGln: 0.397 ± 0.029
0.756MetArg: 0.756 ± 0.04
0.845MetSer: 0.845 ± 0.04
0.614MetThr: 0.614 ± 0.036
1.076MetVal: 1.076 ± 0.051
0.152MetTrp: 0.152 ± 0.016
0.462MetTyr: 0.462 ± 0.03
0.0MetXaa: 0.0 ± 0.0
Asn
1.856AsnAla: 1.856 ± 0.088
0.458AsnCys: 0.458 ± 0.03
1.015AsnAsp: 1.015 ± 0.046
2.654AsnGlu: 2.654 ± 0.071
3.435AsnPhe: 3.435 ± 0.106
1.797AsnGly: 1.797 ± 0.102
0.537AsnHis: 0.537 ± 0.032
4.089AsnIle: 4.089 ± 0.089
3.633AsnLys: 3.633 ± 0.103
6.768AsnLeu: 6.768 ± 0.168
0.707AsnMet: 0.707 ± 0.035
1.601AsnAsn: 1.601 ± 0.077
2.719AsnPro: 2.719 ± 0.066
1.046AsnGln: 1.046 ± 0.052
1.422AsnArg: 1.422 ± 0.054
1.99AsnSer: 1.99 ± 0.077
1.692AsnThr: 1.692 ± 0.055
2.01AsnVal: 2.01 ± 0.072
0.61AsnTrp: 0.61 ± 0.035
2.168AsnTyr: 2.168 ± 0.067
0.0AsnXaa: 0.0 ± 0.0
Pro
1.757ProAla: 1.757 ± 0.063
0.401ProCys: 0.401 ± 0.027
1.88ProAsp: 1.88 ± 0.063
4.658ProGlu: 4.658 ± 0.116
2.798ProPhe: 2.798 ± 0.079
2.509ProGly: 2.509 ± 0.081
0.792ProHis: 0.792 ± 0.037
3.313ProIle: 3.313 ± 0.082
3.682ProLys: 3.682 ± 0.096
4.44ProLeu: 4.44 ± 0.091
0.584ProMet: 0.584 ± 0.038
1.451ProAsn: 1.451 ± 0.057
1.927ProPro: 1.927 ± 0.081
1.414ProGln: 1.414 ± 0.051
1.175ProArg: 1.175 ± 0.056
2.494ProSer: 2.494 ± 0.072
1.755ProThr: 1.755 ± 0.059
2.677ProVal: 2.677 ± 0.084
0.462ProTrp: 0.462 ± 0.036
2.19ProTyr: 2.19 ± 0.073
0.0ProXaa: 0.0 ± 0.0
Gln
1.587GlnAla: 1.587 ± 0.051
0.162GlnCys: 0.162 ± 0.016
0.831GlnAsp: 0.831 ± 0.039
1.963GlnGlu: 1.963 ± 0.068
1.013GlnPhe: 1.013 ± 0.041
1.315GlnGly: 1.315 ± 0.058
0.251GlnHis: 0.251 ± 0.022
2.549GlnIle: 2.549 ± 0.077
2.715GlnLys: 2.715 ± 0.092
1.82GlnLeu: 1.82 ± 0.062
0.424GlnMet: 0.424 ± 0.029
1.052GlnAsn: 1.052 ± 0.051
0.748GlnPro: 0.748 ± 0.039
0.48GlnGln: 0.48 ± 0.036
0.989GlnArg: 0.989 ± 0.048
0.871GlnSer: 0.871 ± 0.041
1.025GlnThr: 1.025 ± 0.046
1.433GlnVal: 1.433 ± 0.055
0.18GlnTrp: 0.18 ± 0.021
0.691GlnTyr: 0.691 ± 0.037
0.0GlnXaa: 0.0 ± 0.0
Arg
2.399ArgAla: 2.399 ± 0.078
0.468ArgCys: 0.468 ± 0.033
1.714ArgAsp: 1.714 ± 0.069
3.641ArgGlu: 3.641 ± 0.095
2.041ArgPhe: 2.041 ± 0.059
2.334ArgGly: 2.334 ± 0.071
0.488ArgHis: 0.488 ± 0.03
3.765ArgIle: 3.765 ± 0.084
3.846ArgLys: 3.846 ± 0.088
3.826ArgLeu: 3.826 ± 0.098
0.642ArgMet: 0.642 ± 0.036
1.552ArgAsn: 1.552 ± 0.053
1.404ArgPro: 1.404 ± 0.062
0.772ArgGln: 0.772 ± 0.043
1.643ArgArg: 1.643 ± 0.062
1.427ArgSer: 1.427 ± 0.063
1.518ArgThr: 1.518 ± 0.056
2.391ArgVal: 2.391 ± 0.072
0.397ArgTrp: 0.397 ± 0.028
1.487ArgTyr: 1.487 ± 0.064
0.0ArgXaa: 0.0 ± 0.0
Ser
3.508SerAla: 3.508 ± 0.096
0.541SerCys: 0.541 ± 0.035
1.884SerAsp: 1.884 ± 0.057
4.03SerGlu: 4.03 ± 0.091
3.781SerPhe: 3.781 ± 0.092
3.291SerGly: 3.291 ± 0.093
0.877SerHis: 0.877 ± 0.039
4.377SerIle: 4.377 ± 0.102
4.428SerLys: 4.428 ± 0.102
6.49SerLeu: 6.49 ± 0.119
0.813SerMet: 0.813 ± 0.042
1.781SerAsn: 1.781 ± 0.061
2.545SerPro: 2.545 ± 0.067
1.86SerGln: 1.86 ± 0.058
1.479SerArg: 1.479 ± 0.053
3.24SerSer: 3.24 ± 0.104
2.073SerThr: 2.073 ± 0.065
2.351SerVal: 2.351 ± 0.069
0.521SerTrp: 0.521 ± 0.034
2.375SerTyr: 2.375 ± 0.071
0.0SerXaa: 0.0 ± 0.0
Thr
2.389ThrAla: 2.389 ± 0.076
0.545ThrCys: 0.545 ± 0.036
1.552ThrAsp: 1.552 ± 0.062
2.839ThrGlu: 2.839 ± 0.079
2.533ThrPhe: 2.533 ± 0.073
3.408ThrGly: 3.408 ± 0.111
0.798ThrHis: 0.798 ± 0.036
3.481ThrIle: 3.481 ± 0.084
3.236ThrLys: 3.236 ± 0.074
5.321ThrLeu: 5.321 ± 0.11
0.638ThrMet: 0.638 ± 0.04
1.526ThrAsn: 1.526 ± 0.091
2.456ThrPro: 2.456 ± 0.083
1.179ThrGln: 1.179 ± 0.053
1.268ThrArg: 1.268 ± 0.051
2.413ThrSer: 2.413 ± 0.078
1.929ThrThr: 1.929 ± 0.079
2.29ThrVal: 2.29 ± 0.071
0.347ThrTrp: 0.347 ± 0.027
1.86ThrTyr: 1.86 ± 0.066
0.0ThrXaa: 0.0 ± 0.0
Val
3.404ValAla: 3.404 ± 0.09
0.817ValCys: 0.817 ± 0.044
2.723ValAsp: 2.723 ± 0.068
4.808ValGlu: 4.808 ± 0.098
3.179ValPhe: 3.179 ± 0.082
3.568ValGly: 3.568 ± 0.099
0.748ValHis: 0.748 ± 0.043
5.489ValIle: 5.489 ± 0.122
5.692ValLys: 5.692 ± 0.111
5.949ValLeu: 5.949 ± 0.124
0.965ValMet: 0.965 ± 0.045
2.197ValAsn: 2.197 ± 0.075
2.344ValPro: 2.344 ± 0.074
0.956ValGln: 0.956 ± 0.04
2.166ValArg: 2.166 ± 0.073
3.349ValSer: 3.349 ± 0.082
2.274ValThr: 2.274 ± 0.073
3.82ValVal: 3.82 ± 0.109
0.531ValTrp: 0.531 ± 0.031
2.288ValTyr: 2.288 ± 0.072
0.0ValXaa: 0.0 ± 0.0
Trp
0.571TrpAla: 0.571 ± 0.035
0.111TrpCys: 0.111 ± 0.017
0.541TrpAsp: 0.541 ± 0.035
1.056TrpGlu: 1.056 ± 0.042
0.486TrpPhe: 0.486 ± 0.028
0.738TrpGly: 0.738 ± 0.04
0.182TrpHis: 0.182 ± 0.018
0.92TrpIle: 0.92 ± 0.047
0.875TrpLys: 0.875 ± 0.042
0.981TrpLeu: 0.981 ± 0.047
0.158TrpMet: 0.158 ± 0.019
0.375TrpAsn: 0.375 ± 0.027
0.197TrpPro: 0.197 ± 0.028
0.294TrpGln: 0.294 ± 0.026
0.419TrpArg: 0.419 ± 0.028
0.34TrpSer: 0.34 ± 0.027
0.28TrpThr: 0.28 ± 0.026
0.59TrpVal: 0.59 ± 0.032
0.13TrpTrp: 0.13 ± 0.015
0.411TrpTyr: 0.411 ± 0.033
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.917TyrAla: 1.917 ± 0.077
0.367TyrCys: 0.367 ± 0.028
1.475TyrAsp: 1.475 ± 0.054
3.21TyrGlu: 3.21 ± 0.087
2.971TyrPhe: 2.971 ± 0.084
2.488TyrGly: 2.488 ± 0.075
0.713TyrHis: 0.713 ± 0.041
3.151TyrIle: 3.151 ± 0.082
3.965TyrLys: 3.965 ± 0.102
5.459TyrLeu: 5.459 ± 0.124
0.559TyrMet: 0.559 ± 0.037
1.682TyrAsn: 1.682 ± 0.067
2.12TyrPro: 2.12 ± 0.065
1.36TyrGln: 1.36 ± 0.051
1.597TyrArg: 1.597 ± 0.053
2.186TyrSer: 2.186 ± 0.064
1.722TyrThr: 1.722 ± 0.056
1.984TyrVal: 1.984 ± 0.064
0.365TyrTrp: 0.365 ± 0.027
2.024TyrTyr: 2.024 ± 0.07
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1594 proteins (506494 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski