Roseivirga ehrenbergii (strain DSM 102268 / JCM 13514 / KCTC 12282 / NCIMB 14502 / KMM 6017)

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales;

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3632 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A150XLH7|A0A150XLH7_ROSEK POTRA domain-containing protein OS=Roseivirga ehrenbergii (strain DSM 102268 / JCM 13514 / KCTC 12282 / NCIMB 14502 / KMM 6017) OX=279360 GN=MB14_17200 PE=4 SV=1
MM1 pKa = 6.9VRR3 pKa = 11.84KK4 pKa = 7.9TRR6 pKa = 11.84ILLLLLGITFSVLTQAQNFTRR27 pKa = 11.84HH28 pKa = 4.91NWYY31 pKa = 10.37FSNNDD36 pKa = 2.85QALIFGKK43 pKa = 9.75EE44 pKa = 3.39QAARR48 pKa = 11.84SFIADD53 pKa = 3.49GKK55 pKa = 9.93IAQTNAGEE63 pKa = 4.52KK64 pKa = 9.13LTATDD69 pKa = 3.89PTTGDD74 pKa = 3.67LLFYY78 pKa = 10.88SDD80 pKa = 5.7GINIYY85 pKa = 10.31DD86 pKa = 3.54ATHH89 pKa = 7.03AIMEE93 pKa = 4.74NGTGLTTDD101 pKa = 3.85PNGIQPLALSPVPGVGNEE119 pKa = 3.23SRR121 pKa = 11.84YY122 pKa = 9.77YY123 pKa = 9.27VFHH126 pKa = 7.11RR127 pKa = 11.84NVTGEE132 pKa = 3.79ILYY135 pKa = 9.12TLVDD139 pKa = 3.71MSSQGNRR146 pKa = 11.84VDD148 pKa = 4.13GPPAGAVVIGSKK160 pKa = 9.42NQPTGIISRR169 pKa = 11.84GDD171 pKa = 3.16GMLTVASGDD180 pKa = 3.41MTQFWLVTQNSTNGNFEE197 pKa = 3.59IHH199 pKa = 7.25EE200 pKa = 4.43IPVAGQPFDD209 pKa = 3.75PQGAPTVLTANVQAAHH225 pKa = 7.02LSLNPMGTQIAVRR238 pKa = 11.84PSNNVNIQILDD249 pKa = 3.71FNRR252 pKa = 11.84DD253 pKa = 3.28TPEE256 pKa = 3.78LMFNRR261 pKa = 11.84SVANSFVPNQTLTGSIGWSNDD282 pKa = 2.38GTKK285 pKa = 10.48LYY287 pKa = 10.67FSRR290 pKa = 11.84NLANSTSGNLYY301 pKa = 10.41RR302 pKa = 11.84IDD304 pKa = 4.88LSDD307 pKa = 3.41PMASVEE313 pKa = 4.32PVFSNPIGASQSVMLAPDD331 pKa = 3.03SSLYY335 pKa = 10.51HH336 pKa = 6.62IYY338 pKa = 10.61KK339 pKa = 8.39EE340 pKa = 4.29TAAGPRR346 pKa = 11.84LLARR350 pKa = 11.84INQPDD355 pKa = 3.72SALTALDD362 pKa = 4.17YY363 pKa = 11.13EE364 pKa = 4.57PSLFDD369 pKa = 3.82EE370 pKa = 5.39FDD372 pKa = 3.59PASDD376 pKa = 3.81YY377 pKa = 10.59FQQFMPPAAIGPTVTISDD395 pKa = 4.07DD396 pKa = 3.47GDD398 pKa = 3.68YY399 pKa = 11.58CMNTPVQFYY408 pKa = 11.34VNIDD412 pKa = 3.55PSTAIPTSYY421 pKa = 10.51SWDD424 pKa = 3.33IQPFGITSNAIAPIFTFDD442 pKa = 3.24QAGMYY447 pKa = 7.39MATLTVEE454 pKa = 4.25FDD456 pKa = 4.19GIGTVMSNTLMGEE469 pKa = 3.51IMEE472 pKa = 4.83NDD474 pKa = 3.31IQVSLQDD481 pKa = 3.37TTICEE486 pKa = 4.58GEE488 pKa = 4.5TLTLDD493 pKa = 4.69AEE495 pKa = 4.44PQSGGQGQQGGATGGPFEE513 pKa = 4.4YY514 pKa = 9.96RR515 pKa = 11.84WNTGEE520 pKa = 4.18TTSSIDD526 pKa = 3.17VTDD529 pKa = 4.11AGNYY533 pKa = 6.32WVVITPTTGCPIYY546 pKa = 9.66EE547 pKa = 4.17TVEE550 pKa = 3.94VKK552 pKa = 10.8VYY554 pKa = 11.03GDD556 pKa = 3.99EE557 pKa = 4.44NPTANIWYY565 pKa = 8.85FGNGAGIDD573 pKa = 3.89FNEE576 pKa = 4.31VDD578 pKa = 4.56GLAPPPRR585 pKa = 11.84SITTAHH591 pKa = 6.83AMNAPEE597 pKa = 4.36GTSTISDD604 pKa = 3.51ANGQVLFYY612 pKa = 11.07TNGTSVWNADD622 pKa = 3.4NGVMPNGTKK631 pKa = 9.86IGGDD635 pKa = 3.57STSTQAVVIVPFEE648 pKa = 4.52DD649 pKa = 5.32DD650 pKa = 3.02EE651 pKa = 4.68TLFYY655 pKa = 11.31VFTTEE660 pKa = 4.73EE661 pKa = 3.92VHH663 pKa = 7.41GDD665 pKa = 3.14NTYY668 pKa = 8.38MLKK671 pKa = 10.75YY672 pKa = 10.47SVVDD676 pKa = 4.09LKK678 pKa = 11.23EE679 pKa = 4.44DD680 pKa = 3.44NGLGAVISKK689 pKa = 10.96DD690 pKa = 3.06NVLFTRR696 pKa = 11.84STEE699 pKa = 3.84KK700 pKa = 10.98LIALEE705 pKa = 4.14GAGGYY710 pKa = 8.97WLMAHH715 pKa = 7.36EE716 pKa = 4.92YY717 pKa = 8.48GTNTFRR723 pKa = 11.84AYY725 pKa = 9.96PITNQGIGQPILSSVGSVHH744 pKa = 7.26SINDD748 pKa = 3.7ALSGQAGMKK757 pKa = 10.41FSGDD761 pKa = 3.23RR762 pKa = 11.84LRR764 pKa = 11.84IAVALIEE771 pKa = 4.38GNEE774 pKa = 4.23DD775 pKa = 3.53YY776 pKa = 11.62VEE778 pKa = 4.45LFSFDD783 pKa = 4.07ANTGEE788 pKa = 4.41VTDD791 pKa = 5.08LLYY794 pKa = 10.89QIDD797 pKa = 4.28LGEE800 pKa = 4.83GGPALNDD807 pKa = 3.12QVYY810 pKa = 10.2DD811 pKa = 3.43VHH813 pKa = 7.54FSTGGDD819 pKa = 3.17KK820 pKa = 11.09LFATMNNRR828 pKa = 11.84NGGAPGGRR836 pKa = 11.84ILEE839 pKa = 3.97YY840 pKa = 10.44RR841 pKa = 11.84IDD843 pKa = 3.78SASTEE848 pKa = 4.25VTRR851 pKa = 11.84LASKK855 pKa = 9.88TDD857 pKa = 2.92ISTSSTVTVNYY868 pKa = 10.34GAIQTGPDD876 pKa = 3.31GQLYY880 pKa = 9.53VAMEE884 pKa = 4.19IPGNPAGTPFIGSINVNEE902 pKa = 4.1DD903 pKa = 3.04TAAVSSFNQMQVPLTTGNSRR923 pKa = 11.84LGLPNFVQNNVNPPMEE939 pKa = 4.93PSMSAPDD946 pKa = 3.64STCVEE951 pKa = 4.04EE952 pKa = 5.47RR953 pKa = 11.84IEE955 pKa = 3.92LSGVGTSDD963 pKa = 2.73IDD965 pKa = 3.54EE966 pKa = 4.6FLWTIIDD973 pKa = 3.99DD974 pKa = 4.22ANTTVFSASGQDD986 pKa = 3.17TAYY989 pKa = 10.8VFPQGQAGRR998 pKa = 11.84FDD1000 pKa = 3.17ISLNISNRR1008 pKa = 11.84CGFDD1012 pKa = 2.92TTFVQTIDD1020 pKa = 3.5VFEE1023 pKa = 4.43IPPRR1027 pKa = 11.84PAVPTAVVLCEE1038 pKa = 4.31GSSHH1042 pKa = 7.47DD1043 pKa = 4.44LDD1045 pKa = 4.52ALGGEE1050 pKa = 4.59PDD1052 pKa = 4.56DD1053 pKa = 4.27PTLSFEE1059 pKa = 4.16WTNSQGAVVSNSRR1072 pKa = 11.84YY1073 pKa = 8.85YY1074 pKa = 10.93SVTEE1078 pKa = 3.8EE1079 pKa = 4.26EE1080 pKa = 5.27IYY1082 pKa = 10.6TITITSVGGGGCVSSAEE1099 pKa = 4.35VYY1101 pKa = 9.81AAPPFEE1107 pKa = 5.63IEE1109 pKa = 4.59LPEE1112 pKa = 4.05TANLCEE1118 pKa = 4.55GEE1120 pKa = 4.66SLTLDD1125 pKa = 3.54PNVNANNYY1133 pKa = 7.51IWTRR1137 pKa = 11.84INPDD1141 pKa = 2.82NSTTTLPNQRR1151 pKa = 11.84TATVDD1156 pKa = 3.17SSVPGIFTYY1165 pKa = 10.59VVSIEE1170 pKa = 4.52DD1171 pKa = 4.86PIEE1174 pKa = 4.39PGCFANDD1181 pKa = 3.49STVVTINGSPNLSLVSSQDD1200 pKa = 3.04ASCPQVGPTPDD1211 pKa = 3.34GEE1213 pKa = 4.64FVFNIAGTGNYY1224 pKa = 9.98SYY1226 pKa = 10.07TVSNATGATVAQSNSVNGPNDD1247 pKa = 3.25VSVTGLDD1254 pKa = 3.33GGIYY1258 pKa = 9.9SVNVTDD1264 pKa = 5.45NSTNCTDD1271 pKa = 3.44EE1272 pKa = 3.99LTGIEE1277 pKa = 3.89IQNNNFNIIEE1287 pKa = 4.24PVVTADD1293 pKa = 3.79ATCDD1297 pKa = 3.42TADD1300 pKa = 3.4GSITITLDD1308 pKa = 3.4DD1309 pKa = 4.29ASVFPVSYY1317 pKa = 11.04VLTRR1321 pKa = 11.84DD1322 pKa = 3.36EE1323 pKa = 5.54AGFTPLSGNAVANGFVISGLEE1344 pKa = 3.8GGTYY1348 pKa = 10.08SIEE1351 pKa = 4.23VTSTALGCTATASGIVVNQPTDD1373 pKa = 3.47VQNLQISGTTEE1384 pKa = 3.57VCDD1387 pKa = 3.69ISPSTTLTATSNNGTNYY1404 pKa = 8.87TWIGPSGPAGNTASITVTEE1423 pKa = 4.33SGTYY1427 pKa = 8.94TVTADD1432 pKa = 3.81APGVACPTSEE1442 pKa = 4.26SVIVTLTVQPDD1453 pKa = 3.45VEE1455 pKa = 4.82IIQIGDD1461 pKa = 3.5VCDD1464 pKa = 3.66GTLVLEE1470 pKa = 4.17AQINNEE1476 pKa = 4.26NPTATYY1482 pKa = 10.72NFAWSTGEE1490 pKa = 3.77NTRR1493 pKa = 11.84SITVSADD1500 pKa = 2.66GTYY1503 pKa = 11.02DD1504 pKa = 3.28VTVRR1508 pKa = 11.84EE1509 pKa = 4.77AGDD1512 pKa = 3.62LTCTGTYY1519 pKa = 10.12SEE1521 pKa = 5.13TIDD1524 pKa = 3.89FPEE1527 pKa = 4.28EE1528 pKa = 3.86LEE1530 pKa = 4.27ATLSSSPACDD1540 pKa = 3.71DD1541 pKa = 3.84GQPITLTVDD1550 pKa = 3.5VLSGNATNFTWTRR1563 pKa = 11.84NGQAAGTGNPLVVNEE1578 pKa = 4.22EE1579 pKa = 3.59GSYY1582 pKa = 9.66TVTISDD1588 pKa = 3.6NSGCSIEE1595 pKa = 4.43RR1596 pKa = 11.84SILIRR1601 pKa = 11.84RR1602 pKa = 11.84SVIPEE1607 pKa = 3.85GLLPDD1612 pKa = 3.97LEE1614 pKa = 5.22YY1615 pKa = 11.46YY1616 pKa = 10.83CSTSSNVLTVTAGIGFEE1633 pKa = 4.29TYY1635 pKa = 8.99EE1636 pKa = 4.15WTLDD1640 pKa = 3.86GQPLAHH1646 pKa = 7.06SDD1648 pKa = 3.36QDD1650 pKa = 4.04LVIAAPGIYY1659 pKa = 9.26QVTMTTATGCVRR1671 pKa = 11.84VDD1673 pKa = 3.11QMEE1676 pKa = 4.73IIEE1679 pKa = 4.46SCDD1682 pKa = 3.52PVILAPNVIVPEE1694 pKa = 4.3GNPPNNVFSVVPNGFVSDD1712 pKa = 3.81FQIFIYY1718 pKa = 10.35SRR1720 pKa = 11.84WGEE1723 pKa = 4.31LIYY1726 pKa = 10.62QSSALEE1732 pKa = 4.09FQWNGTFNGQLVPIGTYY1749 pKa = 9.6PYY1751 pKa = 10.01VIKK1754 pKa = 10.5FRR1756 pKa = 11.84SRR1758 pKa = 11.84FQPEE1762 pKa = 3.49RR1763 pKa = 11.84GEE1765 pKa = 4.07FEE1767 pKa = 3.74QRR1769 pKa = 11.84GAITVVRR1776 pKa = 4.35

Molecular weight:
189.29 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A150X0B7|A0A150X0B7_ROSEK Peptidase OS=Roseivirga ehrenbergii (strain DSM 102268 / JCM 13514 / KCTC 12282 / NCIMB 14502 / KMM 6017) OX=279360 GN=MB14_08900 PE=4 SV=1
MM1 pKa = 7.38TFLFEE6 pKa = 5.33LYY8 pKa = 9.78IKK10 pKa = 10.57RR11 pKa = 11.84LLGKK15 pKa = 9.38YY16 pKa = 9.33RR17 pKa = 11.84YY18 pKa = 9.24RR19 pKa = 11.84RR20 pKa = 11.84KK21 pKa = 10.27SLLWHH26 pKa = 6.81LSRR29 pKa = 11.84GVNCGKK35 pKa = 9.56PLKK38 pKa = 10.4RR39 pKa = 11.84LMEE42 pKa = 4.32TLCSIAHH49 pKa = 6.1GLNRR53 pKa = 11.84GLFEE57 pKa = 6.39CITLGNGFNHH67 pKa = 6.29FNWVVGLLILPQRR80 pKa = 11.84ALSSAKK86 pKa = 10.09GKK88 pKa = 10.01LVQRR92 pKa = 11.84QQ93 pKa = 3.09

Molecular weight:
10.79 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3632

0

3632

1259309

54

4957

346.7

38.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.802 ± 0.037

0.642 ± 0.012

5.576 ± 0.048

6.917 ± 0.05

5.185 ± 0.035

6.809 ± 0.047

1.713 ± 0.021

7.402 ± 0.032

7.01 ± 0.056

9.483 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.412 ± 0.025

5.524 ± 0.037

3.502 ± 0.023

3.509 ± 0.025

3.847 ± 0.029

6.737 ± 0.042

5.57 ± 0.061

6.46 ± 0.029

1.091 ± 0.017

3.809 ± 0.029

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski