Ralstonia phage RSF1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Chiangmaivirus; Ralstonia virus RSF1

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 237 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K2QR61|A0A0K2QR61_9CAUD Uncharacterized protein OS=Ralstonia phage RSF1 OX=1689679 PE=4 SV=1
MM1 pKa = 7.87DD2 pKa = 5.79LSLNLDD8 pKa = 3.56CMINGEE14 pKa = 4.02ADD16 pKa = 3.67FVKK19 pKa = 10.77KK20 pKa = 10.2EE21 pKa = 3.92LEE23 pKa = 4.12YY24 pKa = 11.11LVLAEE29 pKa = 4.06VLAYY33 pKa = 9.5FPNIAAEE40 pKa = 4.35LPEE43 pKa = 4.53DD44 pKa = 4.06MYY46 pKa = 11.12WVTEE50 pKa = 3.91RR51 pKa = 11.84LEE53 pKa = 4.71LIIDD57 pKa = 3.55VPDD60 pKa = 3.79RR61 pKa = 11.84YY62 pKa = 10.38EE63 pKa = 3.68EE64 pKa = 3.77QRR66 pKa = 11.84GYY68 pKa = 11.16FFF70 pKa = 5.88

Molecular weight: 8.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K2QR32|A0A0K2QR32_9CAUD Uncharacterized protein OS=Ralstonia phage RSF1 OX=1689679 PE=4 SV=2
MM1 pKa = 7.53FKK3 pKa = 10.89NILKK7 pKa = 10.33KK8 pKa = 9.3ITFATRR14 pKa = 11.84VNKK17 pKa = 10.19LFAKK21 pKa = 9.98YY22 pKa = 10.62AEE24 pKa = 4.2LKK26 pKa = 9.53AAKK29 pKa = 9.32EE30 pKa = 4.0AARR33 pKa = 11.84TSGISLGGFMAVSKK47 pKa = 10.84KK48 pKa = 9.49IDD50 pKa = 3.33HH51 pKa = 6.6RR52 pKa = 11.84LLRR55 pKa = 11.84IEE57 pKa = 3.95RR58 pKa = 11.84ALYY61 pKa = 10.02RR62 pKa = 11.84VGKK65 pKa = 10.02IDD67 pKa = 3.5TLTFQAAVV75 pKa = 3.25

Molecular weight: 8.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

237

0

237

67278

33

2123

283.9

32.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.754 ± 0.26

0.841 ± 0.061

6.669 ± 0.133

6.391 ± 0.174

4.379 ± 0.109

6.467 ± 0.231

2.112 ± 0.081

5.856 ± 0.117

6.106 ± 0.192

8.26 ± 0.138

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.078 ± 0.082

5.024 ± 0.112

4.255 ± 0.099

4.248 ± 0.113

5.101 ± 0.2

5.824 ± 0.123

5.856 ± 0.164

6.515 ± 0.133

1.284 ± 0.065

3.978 ± 0.104
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski