Aquirufa antheringensis

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Cytophagaceae; Aquirufa

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2203 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q9BHI1|A0A4Q9BHI1_9BACT LamG domain-containing protein OS=Aquirufa antheringensis OX=2516559 GN=EWU20_01575 PE=4 SV=1
MM1 pKa = 7.85SILNNWKK8 pKa = 10.2GFLKK12 pKa = 10.53SFQKK16 pKa = 7.8TTSFLILVVSLILFSGNAHH35 pKa = 6.43AAKK38 pKa = 10.38LYY40 pKa = 11.04FNDD43 pKa = 3.38IYY45 pKa = 11.05KK46 pKa = 9.29ATGSSYY52 pKa = 10.86SIQPSSLDD60 pKa = 3.82GISFISGSGFKK71 pKa = 9.25FTSANPADD79 pKa = 3.88VSFSGNNIAGILSYY93 pKa = 10.74TNSSNQQVSIYY104 pKa = 10.87GVISRR109 pKa = 11.84QNKK112 pKa = 9.27ANGNTMAVNFMPTDD126 pKa = 3.43NTYY129 pKa = 8.2TTVTGEE135 pKa = 4.27GYY137 pKa = 10.43ILVIPSKK144 pKa = 10.65EE145 pKa = 3.75GSFQNGINVATSSDD159 pKa = 4.02PIAAVLNDD167 pKa = 3.37VLTTQNSSPIISINDD182 pKa = 3.16VTVNNTDD189 pKa = 3.0SYY191 pKa = 11.83AVFAITLSRR200 pKa = 11.84TATGITTFTPSLSPATATLNTDD222 pKa = 3.46YY223 pKa = 11.05TNSLQYY229 pKa = 11.1YY230 pKa = 9.63NGSAWVNISSTVSIAQGVTSLQIRR254 pKa = 11.84VPILNAGAVSSRR266 pKa = 11.84KK267 pKa = 9.39FNLNTGAISGSNVLNSDD284 pKa = 3.37GAYY287 pKa = 10.37GIGTILPSPSITTSGTLKK305 pKa = 10.11TFSTCSGCTVNPQAFTVSGSNLTADD330 pKa = 4.41LVVTAPTGVEE340 pKa = 3.92VSTSQNGAYY349 pKa = 9.55QSSVTLSQVSNAVTTTTVYY368 pKa = 10.55TKK370 pKa = 9.62LTNNAVSTSGGTISITSTGAVSKK393 pKa = 9.51TLTVTTNPDD402 pKa = 2.84NALNFDD408 pKa = 3.74GSSDD412 pKa = 3.96YY413 pKa = 11.63VSVPDD418 pKa = 4.19NNKK421 pKa = 10.05LDD423 pKa = 3.82FTTSFTVEE431 pKa = 3.47GWVYY435 pKa = 8.23PTTASSTQVIFGKK448 pKa = 10.59INDD451 pKa = 4.26AGSGDD456 pKa = 3.83GSDD459 pKa = 3.39AALMLRR465 pKa = 11.84LFSGGFRR472 pKa = 11.84VEE474 pKa = 4.23VGKK477 pKa = 8.71GTSPSQFFDD486 pKa = 3.53VNKK489 pKa = 9.14YY490 pKa = 7.47TLNKK494 pKa = 7.41WQHH497 pKa = 5.07IAMVYY502 pKa = 8.28NTGILKK508 pKa = 9.87FYY510 pKa = 10.53INGVEE515 pKa = 3.84EE516 pKa = 4.92GSFNTGYY523 pKa = 11.23GSTPLLNTPSSFKK536 pKa = 10.92FGVFKK541 pKa = 10.37PAFAQYY547 pKa = 10.62FIGSLDD553 pKa = 3.51NFRR556 pKa = 11.84IWDD559 pKa = 3.44IARR562 pKa = 11.84TQSEE566 pKa = 4.32ISSKK570 pKa = 10.26MNEE573 pKa = 4.15EE574 pKa = 4.74LIGTQTGLVASYY586 pKa = 9.19TFNQGIGNGNNSSITTIEE604 pKa = 4.03DD605 pKa = 3.11QTNNGLNGVLNTFSLSGSTSNFISGIIPEE634 pKa = 4.33ISGEE638 pKa = 4.21AILSKK643 pKa = 10.84GATTNYY649 pKa = 9.99TNGLTGGTWSSTDD662 pKa = 3.39TNIATVDD669 pKa = 3.49PTTGVVTGVNAGTSTITYY687 pKa = 6.91TLCDD691 pKa = 3.41KK692 pKa = 10.12TVSKK696 pKa = 10.71LVTVVIPTITTSGSLTTFTTCLGTASVAQTFTVSAQYY733 pKa = 8.95LTANLVLTAPAGYY746 pKa = 9.8EE747 pKa = 3.84LAASSGGTYY756 pKa = 10.58SSTLSIAPSSGSVSARR772 pKa = 11.84LIYY775 pKa = 10.6VRR777 pKa = 11.84LSSASIDD784 pKa = 3.88GQSGTISITSSNAVTQTITTGNASVTSSVGGSISGSASVCSGTNSTVLTLSGEE837 pKa = 4.38TGSITKK843 pKa = 8.37WQSSLTSNFTSVTDD857 pKa = 3.67IASTSASITATNLTATTYY875 pKa = 9.68YY876 pKa = 10.19RR877 pKa = 11.84AVVTLGSCTAATSSVATVTVNALPTVSVNVIPDD910 pKa = 3.41VMTSATSFTVPYY922 pKa = 10.26TNLTVGADD930 pKa = 3.76QYY932 pKa = 11.75SLTAGTTAMTNFVVVSNASVTTSPLTISIPASAQNSYY969 pKa = 11.36DD970 pKa = 3.42FNLTVKK976 pKa = 10.65NSTTGCTSAIVPFVLSVSDD995 pKa = 3.51ITPGTVSGDD1004 pKa = 3.28QSICYY1009 pKa = 9.3GSSPATITSLTDD1021 pKa = 3.29ASGGGSPTYY1030 pKa = 9.93IWQISTNGHH1039 pKa = 6.02SGTYY1043 pKa = 9.01MDD1045 pKa = 5.71IIGATSASYY1054 pKa = 10.05SHH1056 pKa = 7.13PVALTSTTHH1065 pKa = 5.74FRR1067 pKa = 11.84RR1068 pKa = 11.84KK1069 pKa = 8.57ATIGVSFAVTHH1080 pKa = 5.49PVEE1083 pKa = 4.34VLVNALPTIAVSPSSASITTGSSVSLSASGANSYY1117 pKa = 10.44SWTPSTGLSAANTAAVTATPTVTTTYY1143 pKa = 10.16TVTGTNSTTSCSSAATVTVSVNPALTAGTISADD1176 pKa = 3.25QTICVGASPATISSATAATGGTGSIAYY1203 pKa = 8.64VWSSSTNGTTYY1214 pKa = 8.26TTISGATSSTYY1225 pKa = 10.05SPGVISVNTYY1235 pKa = 7.88YY1236 pKa = 10.9KK1237 pKa = 10.44RR1238 pKa = 11.84GASTANDD1245 pKa = 3.2AVIYY1249 pKa = 7.14TSPILITVQSSVGGTIAGSAAVCAGTNSTVLTLSGYY1285 pKa = 8.16TGSVTRR1291 pKa = 11.84WQSALAADD1299 pKa = 4.68FSGIVTNINSVSTSITEE1316 pKa = 4.35TNLTATKK1323 pKa = 10.05YY1324 pKa = 8.38YY1325 pKa = 9.8RR1326 pKa = 11.84AVVRR1330 pKa = 11.84VGVCAASNSATATITVNALPVISISPSAPSITSGSSVTLTASGASTYY1377 pKa = 8.91TWTPSTGLSATNTAVVTASPTVTTTYY1403 pKa = 10.48SVSGTSSSCSSTATVTVTVTTPTPVADD1430 pKa = 3.72TDD1432 pKa = 4.01GDD1434 pKa = 4.36GVTDD1438 pKa = 3.79AQEE1441 pKa = 5.31AIDD1444 pKa = 3.96GTNPNDD1450 pKa = 3.23VCSYY1454 pKa = 10.58LVASQTLTPTSAWSTADD1471 pKa = 3.55CDD1473 pKa = 4.73GDD1475 pKa = 3.72GTPNVTDD1482 pKa = 4.08SDD1484 pKa = 4.39PNDD1487 pKa = 3.94PCVHH1491 pKa = 6.84LAGATPVTTNAIWRR1505 pKa = 11.84AADD1508 pKa = 3.56CDD1510 pKa = 4.09GDD1512 pKa = 3.99GVTNGKK1518 pKa = 8.37EE1519 pKa = 4.28TDD1521 pKa = 4.37DD1522 pKa = 3.74GTDD1525 pKa = 3.47PNEE1528 pKa = 4.16GCSYY1532 pKa = 10.57VVASRR1537 pKa = 11.84TLTPSTAWGTLDD1549 pKa = 4.78CDD1551 pKa = 4.51ADD1553 pKa = 4.12GNSNATDD1560 pKa = 4.07PNIDD1564 pKa = 3.92APVASNDD1571 pKa = 3.58LVNLPSSGALTVNILTNDD1589 pKa = 3.56DD1590 pKa = 4.37FLPGANTEE1598 pKa = 4.24ISRR1601 pKa = 11.84QLPPNDD1607 pKa = 3.32GTAKK1611 pKa = 9.56GTVTFNPLTGEE1622 pKa = 4.09MTYY1625 pKa = 10.87KK1626 pKa = 10.54RR1627 pKa = 11.84APFEE1631 pKa = 4.28TGLVTMGYY1639 pKa = 10.15KK1640 pKa = 8.68VTNKK1644 pKa = 9.91AVSPPVSAFAFVTILACDD1662 pKa = 3.8LQDD1665 pKa = 4.76PLSDD1669 pKa = 3.87CDD1671 pKa = 5.71GDD1673 pKa = 4.22GEE1675 pKa = 5.03PNGTDD1680 pKa = 3.82LAPTDD1685 pKa = 3.62PCVYY1689 pKa = 10.28EE1690 pKa = 3.95PTRR1693 pKa = 11.84QVKK1696 pKa = 10.69ANVSDD1701 pKa = 3.39DD1702 pKa = 3.62WKK1704 pKa = 11.46ALDD1707 pKa = 4.1CDD1709 pKa = 4.04GDD1711 pKa = 4.12GVLNGTEE1718 pKa = 4.17LADD1721 pKa = 3.7GTSPSDD1727 pKa = 3.18ACSFKK1732 pKa = 10.92AGSITLSPSAAWLLLDD1748 pKa = 4.77CDD1750 pKa = 4.32ADD1752 pKa = 3.81GVTNQVEE1759 pKa = 4.66GTQDD1763 pKa = 3.47LDD1765 pKa = 3.61GDD1767 pKa = 4.95GIPNCLDD1774 pKa = 3.25TDD1776 pKa = 3.81SDD1778 pKa = 4.18GDD1780 pKa = 4.34SISDD1784 pKa = 3.54KK1785 pKa = 11.3LEE1787 pKa = 4.4TIVDD1791 pKa = 3.59TDD1793 pKa = 3.71RR1794 pKa = 11.84DD1795 pKa = 4.08GKK1797 pKa = 9.95PDD1799 pKa = 3.9YY1800 pKa = 11.22LDD1802 pKa = 5.15LDD1804 pKa = 4.0SDD1806 pKa = 3.94NDD1808 pKa = 4.66GILDD1812 pKa = 3.67SVEE1815 pKa = 4.5NKK1817 pKa = 9.64VCTGTGILCDD1827 pKa = 3.72TDD1829 pKa = 5.23ADD1831 pKa = 3.95GTPNFRR1837 pKa = 11.84DD1838 pKa = 3.58LDD1840 pKa = 3.89SDD1842 pKa = 3.74ADD1844 pKa = 3.83QIKK1847 pKa = 10.69DD1848 pKa = 3.59VIEE1851 pKa = 4.52ASGSDD1856 pKa = 3.71YY1857 pKa = 11.25NQDD1860 pKa = 3.45GIADD1864 pKa = 3.84GNIDD1868 pKa = 3.66EE1869 pKa = 5.1KK1870 pKa = 11.34GIPSSANKK1878 pKa = 10.33GLTPPDD1884 pKa = 3.37TDD1886 pKa = 4.19RR1887 pKa = 11.84DD1888 pKa = 4.1GKK1890 pKa = 10.83LDD1892 pKa = 4.19PYY1894 pKa = 11.3DD1895 pKa = 3.78VDD1897 pKa = 4.07SDD1899 pKa = 3.99GDD1901 pKa = 4.18GISDD1905 pKa = 3.84FDD1907 pKa = 3.7EE1908 pKa = 5.17EE1909 pKa = 4.61YY1910 pKa = 11.54NEE1912 pKa = 4.12VADD1915 pKa = 6.35FADD1918 pKa = 4.11CDD1920 pKa = 3.56KK1921 pKa = 11.6DD1922 pKa = 4.58GIVNRR1927 pKa = 11.84LDD1929 pKa = 3.74PDD1931 pKa = 3.33EE1932 pKa = 5.71CEE1934 pKa = 3.76IFAPQGISPNNDD1946 pKa = 3.12DD1947 pKa = 4.18KK1948 pKa = 11.8NDD1950 pKa = 3.69RR1951 pKa = 11.84LVFKK1955 pKa = 10.8GLVFRR1960 pKa = 11.84KK1961 pKa = 9.86LPNHH1965 pKa = 6.33LSVYY1969 pKa = 9.67NRR1971 pKa = 11.84WGTLVFEE1978 pKa = 4.79MDD1980 pKa = 4.95DD1981 pKa = 4.2YY1982 pKa = 12.03DD1983 pKa = 4.26NSWSGTILPDD1993 pKa = 3.05GTYY1996 pKa = 10.25FYY1998 pKa = 11.7VLDD2001 pKa = 4.46FYY2003 pKa = 11.5GRR2005 pKa = 11.84KK2006 pKa = 7.8PTISNYY2012 pKa = 10.11LAIDD2016 pKa = 3.57RR2017 pKa = 11.84TIKK2020 pKa = 10.68

Molecular weight: 208.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q9BA72|A0A4Q9BA72_9BACT DNA alkylation repair protein OS=Aquirufa antheringensis OX=2516559 GN=EWU20_09965 PE=4 SV=1
MM1 pKa = 7.41NPTNAKK7 pKa = 9.66NRR9 pKa = 11.84LFLAVGLVGTLCLLSACWSARR30 pKa = 11.84CPYY33 pKa = 7.46QTCRR37 pKa = 11.84VKK39 pKa = 10.52VEE41 pKa = 3.89HH42 pKa = 6.23RR43 pKa = 11.84HH44 pKa = 4.23GVGYY48 pKa = 9.45FRR50 pKa = 11.84PNVAFSWMWTPRR62 pKa = 11.84YY63 pKa = 9.74KK64 pKa = 10.47HH65 pKa = 6.04IRR67 pKa = 11.84ADD69 pKa = 3.53QSKK72 pKa = 10.28KK73 pKa = 10.36KK74 pKa = 10.15PRR76 pKa = 3.57

Molecular weight: 8.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2203

0

2203

789050

31

8145

358.2

39.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.125 ± 0.054

0.774 ± 0.015

5.091 ± 0.066

6.033 ± 0.066

5.067 ± 0.044

7.247 ± 0.056

1.873 ± 0.026

6.949 ± 0.043

6.705 ± 0.066

9.691 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.368 ± 0.031

4.754 ± 0.054

4.017 ± 0.03

3.757 ± 0.036

3.806 ± 0.04

6.68 ± 0.056

5.634 ± 0.102

6.505 ± 0.036

1.209 ± 0.02

3.715 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski