Lentibacillus sp. JNUCC-1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Lentibacillus; unclassified Lentibacillus

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3784 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6N8HR42|A0A6N8HR42_9BACI HTH cro/C1-type domain-containing protein OS=Lentibacillus sp. JNUCC-1 OX=2654513 GN=JNUCC1_02648 PE=4 SV=1
MM1 pKa = 7.65NDD3 pKa = 3.52QIKK6 pKa = 10.55LLIGMVVVIVIPALLAWFIFHH27 pKa = 5.75QQEE30 pKa = 4.43KK31 pKa = 10.45EE32 pKa = 4.07DD33 pKa = 4.36DD34 pKa = 3.57TSQPDD39 pKa = 3.73SKK41 pKa = 11.57SEE43 pKa = 3.89ILWGVDD49 pKa = 2.69SATQADD55 pKa = 3.6QDD57 pKa = 3.91MYY59 pKa = 11.52QCVNSNYY66 pKa = 7.58GTPAVWGRR74 pKa = 11.84YY75 pKa = 9.28LGDD78 pKa = 3.52IEE80 pKa = 4.58NVSDD84 pKa = 5.26ALTKK88 pKa = 10.86DD89 pKa = 3.2EE90 pKa = 5.11AAFLHH95 pKa = 6.36EE96 pKa = 5.3KK97 pKa = 10.32DD98 pKa = 3.65VQILVIYY105 pKa = 10.19NLIEE109 pKa = 4.5DD110 pKa = 3.63ATGYY114 pKa = 10.94EE115 pKa = 4.03NGAAHH120 pKa = 7.19AEE122 pKa = 4.27SAIDD126 pKa = 3.63AANEE130 pKa = 3.66LDD132 pKa = 3.59IPEE135 pKa = 4.5GVALFADD142 pKa = 4.28IEE144 pKa = 4.88PIFPVDD150 pKa = 3.41TAFLEE155 pKa = 4.25SWYY158 pKa = 9.13DD159 pKa = 3.53TLKK162 pKa = 11.04NSAYY166 pKa = 9.5EE167 pKa = 3.86PGVYY171 pKa = 10.28GVFDD175 pKa = 4.23EE176 pKa = 5.59GSDD179 pKa = 3.25ILSSYY184 pKa = 11.72NEE186 pKa = 4.25MEE188 pKa = 4.81PSRR191 pKa = 11.84QQDD194 pKa = 3.83TVVWTAFPQQDD205 pKa = 2.77ITTKK209 pKa = 10.71EE210 pKa = 3.84NAPDD214 pKa = 3.71YY215 pKa = 10.56AAQGPDD221 pKa = 3.0QAKK224 pKa = 10.27VYY226 pKa = 8.94GWQYY230 pKa = 11.26AIDD233 pKa = 4.9GEE235 pKa = 4.78TCTIDD240 pKa = 3.2TNLFKK245 pKa = 10.98PEE247 pKa = 3.57MRR249 pKa = 11.84RR250 pKa = 11.84YY251 pKa = 9.57LWW253 pKa = 3.56

Molecular weight: 28.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6N8HNS8|A0A6N8HNS8_9BACI Serine acetyltransferase OS=Lentibacillus sp. JNUCC-1 OX=2654513 GN=JNUCC1_01885 PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84KK11 pKa = 9.23RR12 pKa = 11.84KK13 pKa = 8.12KK14 pKa = 8.46VHH16 pKa = 5.5GFRR19 pKa = 11.84TRR21 pKa = 11.84MSTKK25 pKa = 10.06NGRR28 pKa = 11.84KK29 pKa = 8.49VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.05GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3784

0

3784

1022484

29

2364

270.2

30.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.517 ± 0.044

0.65 ± 0.014

5.798 ± 0.037

7.252 ± 0.049

4.368 ± 0.029

6.906 ± 0.043

2.403 ± 0.021

7.316 ± 0.038

6.258 ± 0.042

9.461 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.049 ± 0.022

4.149 ± 0.025

3.694 ± 0.027

3.954 ± 0.03

4.252 ± 0.032

5.671 ± 0.027

5.689 ± 0.028

7.084 ± 0.035

1.05 ± 0.015

3.479 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski