Streptomyces sp. 150FB

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7086 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C2B175|A0A0C2B175_9ACTN Dehydratase OS=Streptomyces sp. 150FB OX=1576605 GN=QR77_06690 PE=3 SV=1
MM1 pKa = 7.42TVQQEE6 pKa = 4.13ATADD10 pKa = 3.72GATEE14 pKa = 3.86AQEE17 pKa = 4.03PLEE20 pKa = 4.07VWIDD24 pKa = 3.44QDD26 pKa = 3.99LCTGDD31 pKa = 4.65GICVQYY37 pKa = 10.96APEE40 pKa = 4.18VFEE43 pKa = 5.9LDD45 pKa = 3.05IDD47 pKa = 3.63GLAYY51 pKa = 10.45VKK53 pKa = 10.83SPEE56 pKa = 4.46DD57 pKa = 3.74EE58 pKa = 4.68LLQSPGATTRR68 pKa = 11.84VPLPLLTDD76 pKa = 3.36VRR78 pKa = 11.84DD79 pKa = 3.89SAKK82 pKa = 10.12EE83 pKa = 3.86CPGDD87 pKa = 4.18CIHH90 pKa = 6.28VRR92 pKa = 11.84RR93 pKa = 11.84VSDD96 pKa = 3.47SVEE99 pKa = 4.14VYY101 pKa = 10.93GPDD104 pKa = 3.69AEE106 pKa = 4.31

Molecular weight: 11.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C1Y5Y4|A0A0C1Y5Y4_9ACTN TetR family transcriptional regulator OS=Streptomyces sp. 150FB OX=1576605 GN=QR77_23285 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILATRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.48GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7021

65

7086

2211488

19

6088

312.1

33.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.169 ± 0.037

0.732 ± 0.009

6.099 ± 0.024

5.633 ± 0.028

2.847 ± 0.017

9.511 ± 0.026

2.287 ± 0.015

3.424 ± 0.02

2.265 ± 0.024

10.251 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.8 ± 0.012

1.933 ± 0.016

5.933 ± 0.026

2.801 ± 0.017

7.667 ± 0.035

5.419 ± 0.019

6.208 ± 0.025

8.37 ± 0.028

1.499 ± 0.013

2.148 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski