Desulfallas geothermicus DSM 3669

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Desulfallaceae; Desulfoscipio; Desulfoscipio geothermicus

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3693 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I6ED71|A0A1I6ED71_9FIRM Uncharacterized protein OS=Desulfallas geothermicus DSM 3669 OX=1121426 GN=SAMN05660706_13637 PE=4 SV=1
MM1 pKa = 7.72TDD3 pKa = 3.15QEE5 pKa = 5.02KK6 pKa = 10.91VDD8 pKa = 5.84LINTLHH14 pKa = 6.77PKK16 pKa = 9.61VVDD19 pKa = 3.51YY20 pKa = 11.29DD21 pKa = 3.99ADD23 pKa = 4.07GEE25 pKa = 4.26ICYY28 pKa = 10.2YY29 pKa = 10.88VYY31 pKa = 11.19VPLDD35 pKa = 3.59DD36 pKa = 3.73EE37 pKa = 4.62TRR39 pKa = 11.84DD40 pKa = 3.6VLKK43 pKa = 11.01KK44 pKa = 10.74LGCDD48 pKa = 3.31DD49 pKa = 4.83NYY51 pKa = 10.8INLNSVEE58 pKa = 4.39GLGQLLFDD66 pKa = 4.22LTQVGFDD73 pKa = 3.81FANWWHH79 pKa = 6.24SEE81 pKa = 4.07SGFSLIKK88 pKa = 10.38PIEE91 pKa = 4.18CC92 pKa = 4.76

Molecular weight: 10.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I6DJA9|A0A1I6DJA9_9FIRM Polar amino acid transport system substrate-binding protein OS=Desulfallas geothermicus DSM 3669 OX=1121426 GN=SAMN05660706_11220 PE=4 SV=1
MM1 pKa = 7.33TLNNVMQIHH10 pKa = 5.07EE11 pKa = 4.56RR12 pKa = 11.84KK13 pKa = 9.71RR14 pKa = 11.84LFIGLLVASLVVVSLLAAAIWYY36 pKa = 9.09LVFSPEE42 pKa = 3.49RR43 pKa = 11.84SLVYY47 pKa = 10.53KK48 pKa = 10.57LVLLSVVAVLAAGILLAGFGLAGIVLTIMRR78 pKa = 11.84SRR80 pKa = 11.84PLALLQGPMRR90 pKa = 11.84VALNTFFPLVLILGRR105 pKa = 11.84LFRR108 pKa = 11.84IDD110 pKa = 3.14MNRR113 pKa = 11.84IKK115 pKa = 10.66RR116 pKa = 11.84SFIEE120 pKa = 4.0VNNNLVQAMHH130 pKa = 6.76INAGSHH136 pKa = 5.98QILLLAPHH144 pKa = 7.29CLQNSEE150 pKa = 4.73CVHH153 pKa = 6.22KK154 pKa = 10.64VTGSIDD160 pKa = 2.96NCRR163 pKa = 11.84RR164 pKa = 11.84CGKK167 pKa = 10.42CGVNDD172 pKa = 3.54ILQLRR177 pKa = 11.84DD178 pKa = 3.08RR179 pKa = 11.84YY180 pKa = 10.23GIRR183 pKa = 11.84VGMATGGTLARR194 pKa = 11.84KK195 pKa = 8.61YY196 pKa = 10.66VRR198 pKa = 11.84DD199 pKa = 3.98YY200 pKa = 10.78RR201 pKa = 11.84PKK203 pKa = 10.24AIVAIACEE211 pKa = 4.14RR212 pKa = 11.84DD213 pKa = 3.6LTSGILDD220 pKa = 3.89ANPIPVLGVTNLRR233 pKa = 11.84PNGPCHH239 pKa = 5.48NTQFSLQRR247 pKa = 11.84VDD249 pKa = 4.25EE250 pKa = 4.94AIQYY254 pKa = 7.48FLRR257 pKa = 5.53

Molecular weight: 28.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3693

0

3693

1034905

12

1770

280.2

31.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.836 ± 0.053

1.22 ± 0.018

5.014 ± 0.029

6.783 ± 0.043

3.707 ± 0.029

8.038 ± 0.039

1.874 ± 0.02

6.708 ± 0.044

5.677 ± 0.044

10.066 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.72 ± 0.02

3.928 ± 0.033

4.413 ± 0.026

3.344 ± 0.021

5.868 ± 0.04

4.831 ± 0.025

4.946 ± 0.025

7.858 ± 0.038

0.973 ± 0.016

3.194 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski