Sanxia tombus-like virus 3

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KGL5|A0A1L3KGL5_9VIRU RNA-directed RNA polymerase OS=Sanxia tombus-like virus 3 OX=1923387 PE=4 SV=1
MM1 pKa = 7.62LEE3 pKa = 4.32GVDD6 pKa = 4.08TVPGVYY12 pKa = 9.95PGISPTKK19 pKa = 10.54LPGIPKK25 pKa = 8.83NRR27 pKa = 11.84KK28 pKa = 7.58YY29 pKa = 10.66LTMGQYY35 pKa = 9.58ICDD38 pKa = 3.82YY39 pKa = 10.53EE40 pKa = 4.53VRR42 pKa = 11.84THH44 pKa = 6.77NNSLNNLIRR53 pKa = 11.84GVGEE57 pKa = 3.72RR58 pKa = 11.84VLYY61 pKa = 9.56TDD63 pKa = 5.01KK64 pKa = 11.52NCTPCIKK71 pKa = 9.75PARR74 pKa = 11.84GGIFRR79 pKa = 11.84DD80 pKa = 3.13RR81 pKa = 11.84CGVYY85 pKa = 9.37MRR87 pKa = 11.84TIAQRR92 pKa = 11.84LGHH95 pKa = 5.87QSPVSRR101 pKa = 11.84QQFVEE106 pKa = 5.32FYY108 pKa = 9.45SGRR111 pKa = 11.84RR112 pKa = 11.84RR113 pKa = 11.84AMYY116 pKa = 9.97QKK118 pKa = 10.85AVDD121 pKa = 3.95GLALKK126 pKa = 9.58PIRR129 pKa = 11.84VLDD132 pKa = 3.63SHH134 pKa = 7.15LSTFIKK140 pKa = 10.48AEE142 pKa = 3.91KK143 pKa = 10.42LIFTLKK149 pKa = 9.97PDD151 pKa = 3.98PAPRR155 pKa = 11.84VIQPRR160 pKa = 11.84RR161 pKa = 11.84PVYY164 pKa = 9.43NVEE167 pKa = 3.61VGKK170 pKa = 9.32YY171 pKa = 9.34LRR173 pKa = 11.84PLEE176 pKa = 3.99SRR178 pKa = 11.84IYY180 pKa = 10.95DD181 pKa = 3.87EE182 pKa = 4.53IDD184 pKa = 3.25NIFRR188 pKa = 11.84SPTIMSKK195 pKa = 10.65HH196 pKa = 5.75NSVKK200 pKa = 9.33QAEE203 pKa = 4.59IIVSKK208 pKa = 9.28WQQFQQPACVGLDD221 pKa = 3.07ASRR224 pKa = 11.84FDD226 pKa = 3.47QHH228 pKa = 8.35VSEE231 pKa = 4.21QALKK235 pKa = 10.69FEE237 pKa = 4.63HH238 pKa = 6.58EE239 pKa = 4.77LYY241 pKa = 11.14NSVFKK246 pKa = 10.72CKK248 pKa = 10.1RR249 pKa = 11.84LAKK252 pKa = 10.47LLTWQINNVGHH263 pKa = 7.03ARR265 pKa = 11.84AADD268 pKa = 3.55GKK270 pKa = 9.2FTYY273 pKa = 8.67RR274 pKa = 11.84TKK276 pKa = 10.61GSRR279 pKa = 11.84MSGDD283 pKa = 3.39MNTSLGNKK291 pKa = 9.44FLMCLMCKK299 pKa = 10.19AYY301 pKa = 9.81IDD303 pKa = 3.68TKK305 pKa = 10.42RR306 pKa = 11.84IKK308 pKa = 10.09IDD310 pKa = 3.58FVNNGDD316 pKa = 3.75DD317 pKa = 3.54CLMFLEE323 pKa = 5.14RR324 pKa = 11.84KK325 pKa = 9.4HH326 pKa = 6.81LSQLHH331 pKa = 5.54NLQSYY336 pKa = 8.84FADD339 pKa = 4.06FGFKK343 pKa = 9.37MVTEE347 pKa = 4.34APVFEE352 pKa = 4.89LEE354 pKa = 5.04HH355 pKa = 8.77IEE357 pKa = 4.31FCQSRR362 pKa = 11.84PLKK365 pKa = 11.0CNGIWRR371 pKa = 11.84MTRR374 pKa = 11.84NIRR377 pKa = 11.84TCLARR382 pKa = 11.84DD383 pKa = 3.46VTAVNFGHH391 pKa = 6.53DD392 pKa = 3.36VCMYY396 pKa = 10.09RR397 pKa = 11.84AWLHH401 pKa = 7.06DD402 pKa = 3.33IANCGLAFAGDD413 pKa = 4.01CPVYY417 pKa = 10.58GSFYY421 pKa = 11.11RR422 pKa = 11.84MLLRR426 pKa = 11.84FGVEE430 pKa = 3.91GNYY433 pKa = 9.86HH434 pKa = 6.7DD435 pKa = 5.15RR436 pKa = 11.84HH437 pKa = 5.85DD438 pKa = 4.36AYY440 pKa = 11.22FNVYY444 pKa = 7.97RR445 pKa = 11.84TMSRR449 pKa = 11.84GIDD452 pKa = 3.48VEE454 pKa = 4.48TNQPDD459 pKa = 3.48DD460 pKa = 3.64YY461 pKa = 11.43GRR463 pKa = 11.84YY464 pKa = 8.84SFWMQTGINPDD475 pKa = 3.12AQFEE479 pKa = 4.42LEE481 pKa = 4.21QYY483 pKa = 10.55FDD485 pKa = 3.54NAVWGGDD492 pKa = 2.76KK493 pKa = 10.69RR494 pKa = 11.84QFITNLSRR502 pKa = 11.84LITHH506 pKa = 7.11GSS508 pKa = 3.16

Molecular weight: 58.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KGL5|A0A1L3KGL5_9VIRU RNA-directed RNA polymerase OS=Sanxia tombus-like virus 3 OX=1923387 PE=4 SV=1
MM1 pKa = 7.46APKK4 pKa = 10.15GKK6 pKa = 9.96KK7 pKa = 9.37VSNTVRR13 pKa = 11.84NAVDD17 pKa = 3.04SRR19 pKa = 11.84SRR21 pKa = 11.84GGIRR25 pKa = 11.84PRR27 pKa = 11.84IRR29 pKa = 11.84GTLGNNMVLRR39 pKa = 11.84GNEE42 pKa = 3.74MLEE45 pKa = 4.02NLKK48 pKa = 10.52VPATQTDD55 pKa = 3.26HH56 pKa = 6.86SLGYY60 pKa = 9.47PLIAGVTVARR70 pKa = 11.84DD71 pKa = 3.54SVIFAGVARR80 pKa = 11.84CFQQYY85 pKa = 10.51KK86 pKa = 9.19YY87 pKa = 11.2LPGTALHH94 pKa = 5.79YY95 pKa = 10.4QPSVGLNTSGTIYY108 pKa = 10.68AAWFEE113 pKa = 4.17NPEE116 pKa = 4.27MVSDD120 pKa = 3.83WDD122 pKa = 3.39ILGTVARR129 pKa = 11.84IARR132 pKa = 11.84IKK134 pKa = 10.86ANANVKK140 pKa = 9.73CFPVWQEE147 pKa = 3.1FRR149 pKa = 11.84IPISGTPRR157 pKa = 11.84QKK159 pKa = 10.13MFSTDD164 pKa = 2.14ISTNYY169 pKa = 10.1SVADD173 pKa = 3.64LTRR176 pKa = 11.84TCQGLFAVAIEE187 pKa = 4.24GLTANATAAQTIGRR201 pKa = 11.84CVLEE205 pKa = 4.12TVLQLEE211 pKa = 4.69GLSNPTSS218 pKa = 3.33

Molecular weight: 23.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

726

218

508

363.0

41.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.612 ± 1.505

2.617 ± 0.39

5.096 ± 0.939

3.994 ± 0.162

4.821 ± 0.802

7.3 ± 0.477

2.342 ± 0.71

5.785 ± 0.089

4.959 ± 0.642

7.713 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.893 ± 0.298

5.51 ± 0.226

4.545 ± 0.249

4.408 ± 0.139

7.713 ± 0.415

5.51 ± 0.455

5.923 ± 1.391

6.887 ± 0.682

1.24 ± 0.068

4.132 ± 0.687
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski