Aureimonas sp. Leaf454

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Aurantimonadaceae; Aureimonas; unclassified Aureimonas

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4218 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q6BVB9|A0A0Q6BVB9_9RHIZ Uncharacterized protein OS=Aureimonas sp. Leaf454 OX=1736381 GN=ASG43_13740 PE=3 SV=1
MM1 pKa = 7.36KK2 pKa = 10.37RR3 pKa = 11.84FAILFATTAFVTPAIAADD21 pKa = 3.72VVYY24 pKa = 9.75EE25 pKa = 4.11EE26 pKa = 5.01PPAPVAVMAAPTWTGFYY43 pKa = 10.19IGVQGGGAFNPEE55 pKa = 4.57DD56 pKa = 4.59PDD58 pKa = 3.9SLGIASTFGNATNIGIPNTAGGSLNGAFGGNFASEE93 pKa = 4.12FDD95 pKa = 3.46EE96 pKa = 5.02SFVGGAHH103 pKa = 6.32VGYY106 pKa = 10.06DD107 pKa = 3.45YY108 pKa = 11.86QMDD111 pKa = 3.88NFVLGGVIDD120 pKa = 4.21INALDD125 pKa = 3.86VAQKK129 pKa = 10.5QSAFSNTPAFYY140 pKa = 9.26TAEE143 pKa = 4.08RR144 pKa = 11.84SLDD147 pKa = 3.6FLATARR153 pKa = 11.84LRR155 pKa = 11.84AGYY158 pKa = 10.29AVTPTALAYY167 pKa = 8.63VTGGVAYY174 pKa = 10.44GEE176 pKa = 4.17VDD178 pKa = 3.38YY179 pKa = 11.37SFSSNSPAITAAFPARR195 pKa = 11.84TFEE198 pKa = 4.85DD199 pKa = 3.93EE200 pKa = 5.09DD201 pKa = 4.28DD202 pKa = 3.83FGYY205 pKa = 9.11TVGAGMEE212 pKa = 4.25VMVTEE217 pKa = 4.26NVSFGAEE224 pKa = 3.63YY225 pKa = 10.33LYY227 pKa = 11.03TNLGGDD233 pKa = 3.87SFTTLNGGPFDD244 pKa = 4.6GNGTGLGTGATSFSSNDD261 pKa = 3.2DD262 pKa = 3.53FDD264 pKa = 4.31FHH266 pKa = 6.49TVTAKK271 pKa = 10.46LSYY274 pKa = 10.09RR275 pKa = 11.84FKK277 pKa = 11.26

Molecular weight: 28.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q6BLI2|A0A0Q6BLI2_9RHIZ Formate--tetrahydrofolate ligase OS=Aureimonas sp. Leaf454 OX=1736381 GN=fhs PE=3 SV=1
MM1 pKa = 7.34SASADD6 pKa = 3.37WTDD9 pKa = 4.93DD10 pKa = 3.21GTTMKK15 pKa = 10.82AIDD18 pKa = 3.82ADD20 pKa = 3.78GFEE23 pKa = 4.84ALFSRR28 pKa = 11.84SIDD31 pKa = 2.99PWNYY35 pKa = 6.7RR36 pKa = 11.84TSRR39 pKa = 11.84FEE41 pKa = 3.48AHH43 pKa = 6.6KK44 pKa = 10.76RR45 pKa = 11.84GVLLKK50 pKa = 10.6ACGLGPYY57 pKa = 10.22GRR59 pKa = 11.84GLEE62 pKa = 4.09LACAIGTTTQALSPLCRR79 pKa = 11.84RR80 pKa = 11.84LLAVDD85 pKa = 4.45ASPTAIEE92 pKa = 3.83EE93 pKa = 4.19ARR95 pKa = 11.84AGRR98 pKa = 11.84YY99 pKa = 7.48AKK101 pKa = 10.23PVTFRR106 pKa = 11.84TARR109 pKa = 11.84LPEE112 pKa = 4.34DD113 pKa = 3.74APRR116 pKa = 11.84GPFDD120 pKa = 5.52LIVVSEE126 pKa = 3.92IAYY129 pKa = 8.86YY130 pKa = 10.48LKK132 pKa = 10.59PRR134 pKa = 11.84ALDD137 pKa = 3.61ALTDD141 pKa = 3.74RR142 pKa = 11.84LLAALAPGGRR152 pKa = 11.84IVVLHH157 pKa = 6.45HH158 pKa = 5.64VTPFADD164 pKa = 3.69AAILPARR171 pKa = 11.84AQAQMTARR179 pKa = 11.84LARR182 pKa = 11.84RR183 pKa = 11.84LAPVLSRR190 pKa = 11.84RR191 pKa = 11.84LGRR194 pKa = 11.84FKK196 pKa = 10.62VAAFEE201 pKa = 3.99RR202 pKa = 11.84RR203 pKa = 11.84RR204 pKa = 11.84RR205 pKa = 3.65

Molecular weight: 22.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4218

0

4218

1340498

41

5673

317.8

34.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.332 ± 0.061

0.73 ± 0.012

5.877 ± 0.031

5.894 ± 0.036

3.69 ± 0.027

8.899 ± 0.04

1.892 ± 0.02

4.995 ± 0.026

2.838 ± 0.032

10.174 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.321 ± 0.017

2.163 ± 0.021

5.178 ± 0.03

2.652 ± 0.019

7.631 ± 0.04

5.603 ± 0.029

5.368 ± 0.03

7.623 ± 0.032

1.167 ± 0.015

1.973 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski