Brevibacillus sp. WF146

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Brevibacillus; unclassified Brevibacillus

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3884 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1A5XTW1|A0A1A5XTW1_9BACL Sulfatase domain-containing protein OS=Brevibacillus sp. WF146 OX=319501 GN=A6764_00335 PE=3 SV=1
MM1 pKa = 7.82LEE3 pKa = 3.94ALEE6 pKa = 4.44NRR8 pKa = 11.84IAYY11 pKa = 9.69LRR13 pKa = 11.84GLADD17 pKa = 3.72GLDD20 pKa = 3.83VQEE23 pKa = 4.96HH24 pKa = 5.04SRR26 pKa = 11.84EE27 pKa = 4.07GKK29 pKa = 9.74ILAEE33 pKa = 4.25MIEE36 pKa = 4.54LVDD39 pKa = 4.97DD40 pKa = 4.06IYY42 pKa = 11.5GAFRR46 pKa = 11.84EE47 pKa = 3.98LHH49 pKa = 5.83ARR51 pKa = 11.84VEE53 pKa = 4.13EE54 pKa = 4.11AEE56 pKa = 4.45RR57 pKa = 11.84YY58 pKa = 9.96AEE60 pKa = 5.86AIDD63 pKa = 4.22EE64 pKa = 4.3DD65 pKa = 4.67LEE67 pKa = 4.77DD68 pKa = 3.69VEE70 pKa = 6.15LYY72 pKa = 11.17LFGDD76 pKa = 4.05DD77 pKa = 3.39EE78 pKa = 4.46NLYY81 pKa = 8.96EE82 pKa = 4.56TVGDD86 pKa = 3.93CAAEE90 pKa = 3.81EE91 pKa = 4.28RR92 pKa = 11.84EE93 pKa = 4.34AYY95 pKa = 9.28EE96 pKa = 4.98AFADD100 pKa = 5.0LDD102 pKa = 4.28DD103 pKa = 6.56DD104 pKa = 4.26EE105 pKa = 6.1DD106 pKa = 5.4AYY108 pKa = 11.37LYY110 pKa = 7.54EE111 pKa = 4.49TGGRR115 pKa = 11.84DD116 pKa = 3.42HH117 pKa = 6.43VTSSHH122 pKa = 7.02AIEE125 pKa = 4.93CPSCRR130 pKa = 11.84EE131 pKa = 3.45VLFFHH136 pKa = 7.08EE137 pKa = 5.13GVDD140 pKa = 3.84DD141 pKa = 4.23EE142 pKa = 5.92GYY144 pKa = 9.45HH145 pKa = 6.28HH146 pKa = 6.97YY147 pKa = 10.31IVEE150 pKa = 4.65PYY152 pKa = 10.34LDD154 pKa = 3.61EE155 pKa = 6.66AEE157 pKa = 4.42YY158 pKa = 11.1EE159 pKa = 4.48PINPTT164 pKa = 3.34

Molecular weight: 18.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1A5WY08|A0A1A5WY08_9BACL 2' 3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase OS=Brevibacillus sp. WF146 OX=319501 GN=A6764_19115 PE=3 SV=1
MM1 pKa = 7.67SGRR4 pKa = 11.84NLIGVIAASGLIAAIGFMVAPRR26 pKa = 11.84RR27 pKa = 11.84RR28 pKa = 11.84RR29 pKa = 11.84RR30 pKa = 11.84RR31 pKa = 11.84FGFRR35 pKa = 11.84FNRR38 pKa = 11.84LPVSLRR44 pKa = 11.84NVMNVINMSRR54 pKa = 11.84TLLRR58 pKa = 11.84AVARR62 pKa = 4.23

Molecular weight: 7.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3884

0

3884

1149012

27

2726

295.8

32.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.337 ± 0.052

0.82 ± 0.011

4.952 ± 0.033

6.999 ± 0.042

3.939 ± 0.028

7.652 ± 0.036

2.208 ± 0.019

5.987 ± 0.034

4.864 ± 0.038

10.321 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.688 ± 0.019

3.008 ± 0.028

4.435 ± 0.026

3.841 ± 0.027

6.179 ± 0.048

5.179 ± 0.027

5.231 ± 0.028

8.004 ± 0.033

1.207 ± 0.016

3.148 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski