Streptococcus phage phiJH1301-3

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 24 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X9I5U5|A0A1X9I5U5_9VIRU HTH DNA-binding protein OS=Streptococcus phage phiJH1301-3 OX=1860190 PE=4 SV=1
MM1 pKa = 6.75TLKK4 pKa = 10.1TISDD8 pKa = 3.77TANTFTFNYY17 pKa = 8.23TFKK20 pKa = 11.31DD21 pKa = 3.13NDD23 pKa = 3.71TAQVAGHH30 pKa = 6.91ALMGYY35 pKa = 6.39MTGTFEE41 pKa = 4.07QPAIEE46 pKa = 4.14VTYY49 pKa = 11.2LNDD52 pKa = 3.26EE53 pKa = 4.02QGANRR58 pKa = 11.84LAVEE62 pKa = 4.16YY63 pKa = 10.95VADD66 pKa = 4.0TEE68 pKa = 4.17LTEE71 pKa = 4.19TFKK74 pKa = 10.98RR75 pKa = 11.84ICDD78 pKa = 3.76SFKK81 pKa = 10.98DD82 pKa = 4.08YY83 pKa = 11.57YY84 pKa = 11.44NNPDD88 pKa = 3.69EE89 pKa = 4.43LTGEE93 pKa = 4.53DD94 pKa = 3.7FDD96 pKa = 7.02DD97 pKa = 4.59YY98 pKa = 11.39EE99 pKa = 4.33QEE101 pKa = 3.99QKK103 pKa = 10.29IEE105 pKa = 3.82QEE107 pKa = 4.39YY108 pKa = 9.43IRR110 pKa = 11.84QRR112 pKa = 11.84VEE114 pKa = 3.48QLKK117 pKa = 10.15QSEE120 pKa = 4.74DD121 pKa = 3.48FDD123 pKa = 4.65SLLKK127 pKa = 10.55KK128 pKa = 10.52VAGLEE133 pKa = 4.09LEE135 pKa = 4.31LMEE138 pKa = 5.58LADD141 pKa = 5.2SVLDD145 pKa = 4.48DD146 pKa = 5.79DD147 pKa = 4.82YY148 pKa = 11.86PDD150 pKa = 3.45MAVNGVWEE158 pKa = 4.4NMTAVDD164 pKa = 4.32DD165 pKa = 4.27EE166 pKa = 4.24ARR168 pKa = 11.84KK169 pKa = 9.35LLKK172 pKa = 10.67EE173 pKa = 4.53LDD175 pKa = 3.8TEE177 pKa = 4.92DD178 pKa = 4.53NYY180 pKa = 11.24CALWKK185 pKa = 10.72YY186 pKa = 10.33SAEE189 pKa = 4.08

Molecular weight: 21.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X9I5Y5|A0A1X9I5Y5_9VIRU Integrase/recombinase OS=Streptococcus phage phiJH1301-3 OX=1860190 PE=3 SV=1
MM1 pKa = 7.42ARR3 pKa = 11.84YY4 pKa = 9.44SIHH7 pKa = 6.69PADD10 pKa = 4.59SSGHH14 pKa = 4.03YY15 pKa = 10.53HH16 pKa = 6.97NIKK19 pKa = 9.9LYY21 pKa = 10.27KK22 pKa = 9.94DD23 pKa = 3.39RR24 pKa = 11.84RR25 pKa = 11.84PTFEE29 pKa = 3.95QVKK32 pKa = 8.94EE33 pKa = 3.92QQRR36 pKa = 11.84LKK38 pKa = 10.7KK39 pKa = 10.24LKK41 pKa = 10.01KK42 pKa = 9.67KK43 pKa = 9.88RR44 pKa = 11.84RR45 pKa = 11.84KK46 pKa = 9.34

Molecular weight: 5.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

24

0

24

3929

42

533

163.7

18.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.312 ± 0.269

0.636 ± 0.197

5.65 ± 0.413

8.094 ± 0.664

4.123 ± 0.372

5.192 ± 0.341

2.214 ± 0.315

6.77 ± 0.318

8.501 ± 0.497

9.748 ± 0.461

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.392 ± 0.222

4.429 ± 0.278

3.258 ± 0.508

4.632 ± 0.35

5.447 ± 0.343

5.167 ± 0.376

6.643 ± 0.478

5.294 ± 0.375

1.094 ± 0.151

4.403 ± 0.296
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski