Spinach cryptic virus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Duplopiviricetes; Durnavirales; Partitiviridae; unclassified Partitiviridae

Average proteome isoelectric point is 7.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1P8NQ96|A0A1P8NQ96_9VIRU RNA-dependent RNA polymerase OS=Spinach cryptic virus 1 OX=1937817 GN=RdRp PE=4 SV=1
MM1 pKa = 7.49NPEE4 pKa = 4.14TPLADD9 pKa = 4.09PNGPTPPHH17 pKa = 6.31GAAAQPTPGQHH28 pKa = 6.07IPAGTPSTGAVHH40 pKa = 6.59LTPAPQPKK48 pKa = 9.75RR49 pKa = 11.84NRR51 pKa = 11.84APRR54 pKa = 11.84GPFDD58 pKa = 3.76NASSTSAPGAPALLEE73 pKa = 3.95LAAAYY78 pKa = 9.21PMSTEE83 pKa = 3.91QRR85 pKa = 11.84RR86 pKa = 11.84SPNTFVPDD94 pKa = 3.45AQMLFHH100 pKa = 6.92VLSLCDD106 pKa = 4.12NMMLSTDD113 pKa = 3.42RR114 pKa = 11.84FTRR117 pKa = 11.84SAPAWLPIVSQLYY130 pKa = 10.17ISILWFTMILKK141 pKa = 10.32VYY143 pKa = 10.14VNSSYY148 pKa = 11.54GAMHH152 pKa = 6.98AHH154 pKa = 7.6LLNILVAHH162 pKa = 6.31LRR164 pKa = 11.84IDD166 pKa = 3.39EE167 pKa = 4.21CLIPGPLVPFFQSLGAINGPFEE189 pKa = 4.86WIGDD193 pKa = 3.76IVPIIPEE200 pKa = 4.79FSQLWNTTSFYY211 pKa = 9.92PTDD214 pKa = 3.18SFARR218 pKa = 11.84IFPIPAILLDD228 pKa = 3.6QLHH231 pKa = 6.19YY232 pKa = 9.92FASITVTEE240 pKa = 4.02QEE242 pKa = 4.4TIFGHH247 pKa = 5.62FEE249 pKa = 3.51WYY251 pKa = 10.4RR252 pKa = 11.84NIFSLGQGTFNPRR265 pKa = 11.84LRR267 pKa = 11.84LGPQLCGSLYY277 pKa = 9.56ATEE280 pKa = 4.24SQVTNARR287 pKa = 11.84TFWSAALRR295 pKa = 11.84TGITRR300 pKa = 11.84TNAAANQPLFSSYY313 pKa = 10.19PQLLGFVDD321 pKa = 4.34QNDD324 pKa = 3.29AMQLDD329 pKa = 5.02WFQHH333 pKa = 4.56TAIVMQKK340 pKa = 8.62YY341 pKa = 8.82CQYY344 pKa = 10.77FNGSVPLKK352 pKa = 10.26SISPTGIGAVAVHH365 pKa = 6.93GYY367 pKa = 9.75PNYY370 pKa = 10.71SGAARR375 pKa = 11.84TWMYY379 pKa = 10.17PATNTLSSFKK389 pKa = 10.48SSRR392 pKa = 11.84FSALRR397 pKa = 11.84EE398 pKa = 3.96IPALLTVRR406 pKa = 11.84FEE408 pKa = 4.42HH409 pKa = 7.19ADD411 pKa = 3.49HH412 pKa = 6.48EE413 pKa = 4.53LEE415 pKa = 4.27EE416 pKa = 4.19QAEE419 pKa = 4.35QYY421 pKa = 11.19AIATHH426 pKa = 6.91TNMCWSKK433 pKa = 11.38NLTTQNNWTQVAEE446 pKa = 4.35ANVHH450 pKa = 6.18SGTYY454 pKa = 9.53WSMTPHH460 pKa = 7.14RR461 pKa = 11.84AHH463 pKa = 6.79ASVRR467 pKa = 11.84LKK469 pKa = 9.2TQYY472 pKa = 10.88AQLVASRR479 pKa = 11.84YY480 pKa = 8.33HH481 pKa = 4.53QQAANRR487 pKa = 11.84VV488 pKa = 3.59

Molecular weight: 54.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1P8NQ96|A0A1P8NQ96_9VIRU RNA-dependent RNA polymerase OS=Spinach cryptic virus 1 OX=1937817 GN=RdRp PE=4 SV=1
MM1 pKa = 8.32DD2 pKa = 4.54YY3 pKa = 9.87LTSAFNRR10 pKa = 11.84ITHH13 pKa = 7.05WFTKK17 pKa = 8.13PTNLEE22 pKa = 4.11YY23 pKa = 10.41IGDD26 pKa = 4.0YY27 pKa = 8.46STPPSIIRR35 pKa = 11.84VNEE38 pKa = 3.64VAIANHH44 pKa = 6.41KK45 pKa = 8.46KK46 pKa = 6.56TLRR49 pKa = 11.84YY50 pKa = 9.94AFEE53 pKa = 4.03QYY55 pKa = 10.84LYY57 pKa = 9.86DD58 pKa = 3.33HH59 pKa = 8.11EE60 pKa = 4.34YY61 pKa = 11.34RR62 pKa = 11.84MIVDD66 pKa = 4.18EE67 pKa = 4.34YY68 pKa = 10.64RR69 pKa = 11.84RR70 pKa = 11.84SDD72 pKa = 3.1IDD74 pKa = 3.44QEE76 pKa = 4.71SILADD81 pKa = 4.36FFSGDD86 pKa = 3.85IEE88 pKa = 4.44PFDD91 pKa = 3.74IPFDD95 pKa = 3.52EE96 pKa = 4.69HH97 pKa = 8.08VEE99 pKa = 3.99HH100 pKa = 7.35GLRR103 pKa = 11.84CMANAFRR110 pKa = 11.84PPRR113 pKa = 11.84PCRR116 pKa = 11.84PAHH119 pKa = 6.19ILDD122 pKa = 3.78VKK124 pKa = 10.61HH125 pKa = 6.44GYY127 pKa = 8.11PYY129 pKa = 10.43KK130 pKa = 10.06WNVNAEE136 pKa = 4.45PPFSTDD142 pKa = 3.47PYY144 pKa = 10.61FLSQRR149 pKa = 11.84KK150 pKa = 6.53TFGEE154 pKa = 4.98FIQMHH159 pKa = 6.4EE160 pKa = 4.3YY161 pKa = 10.66EE162 pKa = 5.81HH163 pKa = 6.97IDD165 pKa = 3.09KK166 pKa = 11.16ADD168 pKa = 3.68FFRR171 pKa = 11.84RR172 pKa = 11.84HH173 pKa = 6.43PNTEE177 pKa = 3.52SHH179 pKa = 7.34DD180 pKa = 3.85FLTTVVPPKK189 pKa = 10.49FGYY192 pKa = 9.69LKK194 pKa = 11.1SMVFSWTRR202 pKa = 11.84RR203 pKa = 11.84WHH205 pKa = 6.16HH206 pKa = 5.75VIKK209 pKa = 9.97TGFQDD214 pKa = 3.5LTGLEE219 pKa = 4.16NSGYY223 pKa = 10.32LYY225 pKa = 10.98NRR227 pKa = 11.84FIFPMLLHH235 pKa = 6.66TKK237 pKa = 8.3TAIVKK242 pKa = 10.57KK243 pKa = 10.26NDD245 pKa = 3.58PNKK248 pKa = 9.91MRR250 pKa = 11.84TIWGAPKK257 pKa = 9.48PWIIAEE263 pKa = 4.17TMLYY267 pKa = 9.2WEE269 pKa = 4.49YY270 pKa = 10.8IAWIKK275 pKa = 10.52LNSGKK280 pKa = 8.34TPMLWGFEE288 pKa = 4.25TFTGGWFRR296 pKa = 11.84LNNILFCGLIKK307 pKa = 10.6QSFLTLDD314 pKa = 3.5WSRR317 pKa = 11.84FDD319 pKa = 3.08KK320 pKa = 10.75RR321 pKa = 11.84AYY323 pKa = 10.1FSLLRR328 pKa = 11.84RR329 pKa = 11.84ILYY332 pKa = 6.74TARR335 pKa = 11.84TFLTFDD341 pKa = 2.91EE342 pKa = 6.09GYY344 pKa = 10.31VPTHH348 pKa = 7.06AAPTHH353 pKa = 5.97PKK355 pKa = 9.28WNHH358 pKa = 3.88QKK360 pKa = 11.04ALNLEE365 pKa = 4.43RR366 pKa = 11.84LWLWTLEE373 pKa = 4.18NLFEE377 pKa = 4.94APIILPDD384 pKa = 2.89GRR386 pKa = 11.84MYY388 pKa = 10.65RR389 pKa = 11.84RR390 pKa = 11.84HH391 pKa = 5.78FAGIPSGLFITQLLDD406 pKa = 2.7SWYY409 pKa = 9.85NYY411 pKa = 9.33TMLATLLSALGFDD424 pKa = 4.3PKK426 pKa = 11.16YY427 pKa = 10.85CIIKK431 pKa = 9.83VQGDD435 pKa = 3.45DD436 pKa = 4.79SIIKK440 pKa = 8.74LTTLIPRR447 pKa = 11.84DD448 pKa = 3.31QHH450 pKa = 6.01EE451 pKa = 4.46NFMVQLTALATTYY464 pKa = 10.69FNATINVKK472 pKa = 10.27KK473 pKa = 10.86SEE475 pKa = 4.06IRR477 pKa = 11.84NTLNGCEE484 pKa = 3.86VLSYY488 pKa = 10.75KK489 pKa = 10.05NHH491 pKa = 6.42NGFPHH496 pKa = 6.91RR497 pKa = 11.84DD498 pKa = 4.01EE499 pKa = 4.17ITMLAQFYY507 pKa = 7.85HH508 pKa = 6.12TKK510 pKa = 10.61ARR512 pKa = 11.84DD513 pKa = 3.58PTPEE517 pKa = 3.33ITMAQAIGFAYY528 pKa = 10.22ASCGNNKK535 pKa = 9.26RR536 pKa = 11.84VLWILRR542 pKa = 11.84DD543 pKa = 3.15IYY545 pKa = 11.37NFYY548 pKa = 11.2KK549 pKa = 10.24MRR551 pKa = 11.84DD552 pKa = 3.55VTPNRR557 pKa = 11.84AGLTLTFGDD566 pKa = 4.2SPDD569 pKa = 3.21VFMPEE574 pKa = 3.47IPLDD578 pKa = 3.71HH579 pKa = 7.05FPTITEE585 pKa = 3.31IRR587 pKa = 11.84RR588 pKa = 11.84YY589 pKa = 7.58LTCSEE594 pKa = 4.24YY595 pKa = 10.89RR596 pKa = 11.84NEE598 pKa = 4.15AQDD601 pKa = 3.1ARR603 pKa = 11.84TWPRR607 pKa = 11.84SLFISGPAEE616 pKa = 3.67

Molecular weight: 72.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1104

488

616

552.0

63.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.062 ± 1.785

1.087 ± 0.04

4.257 ± 1.016

4.438 ± 0.609

6.159 ± 0.661

4.801 ± 0.205

3.714 ± 0.016

6.25 ± 0.719

3.351 ± 1.222

8.877 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.536 ± 0.049

5.072 ± 0.032

7.246 ± 0.737

3.804 ± 1.233

5.707 ± 0.633

5.888 ± 1.211

7.971 ± 0.013

3.714 ± 0.507

2.536 ± 0.18

4.529 ± 0.536
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski