Gordonia phage Getalong

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Langleyhallvirinae; Getalongvirus; Gordonia virus Getalong

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 104 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A386KE70|A0A386KE70_9CAUD Uncharacterized protein OS=Gordonia phage Getalong OX=2315531 GN=8 PE=4 SV=1
MM1 pKa = 7.35TLYY4 pKa = 10.89DD5 pKa = 3.34VDD7 pKa = 4.42VYY9 pKa = 10.71TKK11 pKa = 10.46VRR13 pKa = 11.84EE14 pKa = 4.44TYY16 pKa = 10.09CVEE19 pKa = 3.66ASTPEE24 pKa = 3.54EE25 pKa = 4.05AAANWMDD32 pKa = 3.71GDD34 pKa = 4.57LMGSEE39 pKa = 4.78CIEE42 pKa = 4.04VEE44 pKa = 4.25DD45 pKa = 3.59VGEE48 pKa = 4.09AKK50 pKa = 10.67VVDD53 pKa = 4.1EE54 pKa = 4.62

Molecular weight: 6.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A386KG25|A0A386KG25_9CAUD Head-to-tail adaptor OS=Gordonia phage Getalong OX=2315531 GN=9 PE=4 SV=1
MM1 pKa = 8.25DD2 pKa = 4.89KK3 pKa = 10.33PICISDD9 pKa = 3.85GCSNPAPYY17 pKa = 10.24LPKK20 pKa = 10.54LKK22 pKa = 10.48RR23 pKa = 11.84CGACHH28 pKa = 5.6YY29 pKa = 9.66RR30 pKa = 11.84WQRR33 pKa = 11.84EE34 pKa = 3.65HH35 pKa = 6.77GKK37 pKa = 9.44RR38 pKa = 11.84HH39 pKa = 5.07TATCVVCGVEE49 pKa = 4.3YY50 pKa = 10.75SRR52 pKa = 11.84SRR54 pKa = 11.84KK55 pKa = 8.82PRR57 pKa = 11.84GRR59 pKa = 11.84PCCSPACRR67 pKa = 11.84QALAQPEE74 pKa = 4.21ATLASAATATQRR86 pKa = 11.84AAEE89 pKa = 4.19RR90 pKa = 11.84RR91 pKa = 11.84RR92 pKa = 11.84EE93 pKa = 4.02RR94 pKa = 11.84YY95 pKa = 8.57GVAVVPYY102 pKa = 8.7VKK104 pKa = 9.9PRR106 pKa = 11.84RR107 pKa = 11.84AWNPTHH113 pKa = 6.1TSGRR117 pKa = 11.84VTWRR121 pKa = 11.84SGPCRR126 pKa = 11.84VCGKK130 pKa = 10.03HH131 pKa = 3.96YY132 pKa = 6.71TTWNIDD138 pKa = 3.55VTCSPEE144 pKa = 3.64CHH146 pKa = 6.88AIRR149 pKa = 11.84LRR151 pKa = 11.84EE152 pKa = 3.68VRR154 pKa = 11.84LVHH157 pKa = 6.37KK158 pKa = 10.46ARR160 pKa = 11.84RR161 pKa = 11.84RR162 pKa = 11.84ARR164 pKa = 11.84KK165 pKa = 9.02RR166 pKa = 11.84DD167 pKa = 3.26AYY169 pKa = 10.68RR170 pKa = 11.84ADD172 pKa = 3.31VHH174 pKa = 6.38RR175 pKa = 11.84KK176 pKa = 9.05RR177 pKa = 11.84VFEE180 pKa = 3.77MDD182 pKa = 3.83GYY184 pKa = 10.85RR185 pKa = 11.84CHH187 pKa = 7.47LCGKK191 pKa = 8.62KK192 pKa = 8.46TMRR195 pKa = 11.84TKK197 pKa = 9.97TVPHH201 pKa = 6.86PKK203 pKa = 10.3APTVDD208 pKa = 4.06HH209 pKa = 7.35IIPLAAGGTHH219 pKa = 6.14EE220 pKa = 4.96PSNCRR225 pKa = 11.84TACFQCNAIKK235 pKa = 9.92SHH237 pKa = 6.48KK238 pKa = 10.58GGGEE242 pKa = 3.77QLILLL247 pKa = 4.53

Molecular weight: 27.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

104

0

104

17824

40

1877

171.4

18.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.092 ± 0.435

1.403 ± 0.27

6.474 ± 0.3

5.784 ± 0.302

2.727 ± 0.202

8.242 ± 0.388

2.25 ± 0.198

4.589 ± 0.189

3.658 ± 0.31

7.647 ± 0.272

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.401 ± 0.171

2.844 ± 0.197

5.425 ± 0.203

3.652 ± 0.133

7.428 ± 0.339

5.874 ± 0.238

6.261 ± 0.337

7.653 ± 0.312

2.261 ± 0.123

2.334 ± 0.172
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski