Alfalfa associated nucleorhabdovirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Nucleorhabdovirus; unclassified Nucleorhabdovirus

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A451G5G6|A0A451G5G6_9RHAB Phosphoprotein OS=Alfalfa associated nucleorhabdovirus OX=2518374 GN=P PE=4 SV=1
MM1 pKa = 6.8STTRR5 pKa = 11.84IDD7 pKa = 3.39NSGWDD12 pKa = 3.98SEE14 pKa = 4.7DD15 pKa = 3.56SMEE18 pKa = 4.45GVCFDD23 pKa = 3.52IHH25 pKa = 8.01DD26 pKa = 4.23GDD28 pKa = 4.16MIIPVKK34 pKa = 10.44FIINDD39 pKa = 3.88LVRR42 pKa = 11.84DD43 pKa = 3.81IFKK46 pKa = 11.07APDD49 pKa = 3.24NSDD52 pKa = 4.02SIRR55 pKa = 11.84EE56 pKa = 3.88PTLNNKK62 pKa = 8.99AAEE65 pKa = 4.3HH66 pKa = 6.55GEE68 pKa = 4.01DD69 pKa = 3.82PEE71 pKa = 4.44KK72 pKa = 11.12KK73 pKa = 8.07EE74 pKa = 4.38TEE76 pKa = 4.27PTTHH80 pKa = 7.3DD81 pKa = 3.64SVHH84 pKa = 6.38EE85 pKa = 4.19KK86 pKa = 9.4MQTTEE91 pKa = 4.47PEE93 pKa = 4.08DD94 pKa = 3.68AAPTSPSVPCSGTEE108 pKa = 4.04SASSNN113 pKa = 3.38

Molecular weight: 12.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A410HY56|A0A410HY56_9RHAB Glycoprotein OS=Alfalfa associated nucleorhabdovirus OX=2518374 GN=G PE=4 SV=1
MM1 pKa = 6.9TAPRR5 pKa = 11.84TMNDD9 pKa = 2.98KK10 pKa = 10.97KK11 pKa = 10.95KK12 pKa = 10.16DD13 pKa = 3.5TLLPTKK19 pKa = 10.35KK20 pKa = 9.17RR21 pKa = 11.84TSQDD25 pKa = 2.55KK26 pKa = 9.76YY27 pKa = 10.28NFRR30 pKa = 11.84STEE33 pKa = 3.78SLYY36 pKa = 11.05AEE38 pKa = 5.28PYY40 pKa = 10.08NKK42 pKa = 9.69IIRR45 pKa = 11.84TKK47 pKa = 10.89DD48 pKa = 3.04CLLPQRR54 pKa = 11.84TLMWFLSLTDD64 pKa = 3.32QSIEE68 pKa = 3.88TRR70 pKa = 11.84IEE72 pKa = 3.95KK73 pKa = 10.26IKK75 pKa = 9.57ITWHH79 pKa = 6.37PSVLPDD85 pKa = 3.76PKK87 pKa = 10.72KK88 pKa = 10.48KK89 pKa = 9.26IHH91 pKa = 6.9IMIKK95 pKa = 10.16DD96 pKa = 3.44RR97 pKa = 11.84EE98 pKa = 4.33EE99 pKa = 4.41YY100 pKa = 10.14SVHH103 pKa = 6.89SSAIYY108 pKa = 10.48SNGDD112 pKa = 3.1NTVLHH117 pKa = 6.58AVGLLSEE124 pKa = 4.45MVCVEE129 pKa = 4.09WSPCHH134 pKa = 7.15SYY136 pKa = 10.5ILEE139 pKa = 4.44RR140 pKa = 11.84NDD142 pKa = 3.25RR143 pKa = 11.84TILPWVVRR151 pKa = 11.84IEE153 pKa = 3.83HH154 pKa = 6.27DD155 pKa = 3.39QRR157 pKa = 11.84SVLEE161 pKa = 4.71DD162 pKa = 3.39GVSFGTVEE170 pKa = 3.67ITGVIHH176 pKa = 7.0CARR179 pKa = 11.84SHH181 pKa = 6.12KK182 pKa = 10.22GVPRR186 pKa = 11.84TIPFSEE192 pKa = 3.95PRR194 pKa = 11.84VLGSNINFPYY204 pKa = 10.38YY205 pKa = 10.19SPCFIGRR212 pKa = 11.84GARR215 pKa = 11.84SNYY218 pKa = 9.22KK219 pKa = 10.29RR220 pKa = 11.84IGVSNCQEE228 pKa = 3.75DD229 pKa = 4.1AFEE232 pKa = 4.97KK233 pKa = 10.61EE234 pKa = 3.81IMPYY238 pKa = 9.92LPKK241 pKa = 10.6DD242 pKa = 3.49ISPKK246 pKa = 10.58GLWKK250 pKa = 10.37LKK252 pKa = 10.67CLITDD257 pKa = 3.08QDD259 pKa = 3.3KK260 pKa = 11.26RR261 pKa = 11.84DD262 pKa = 3.98IISCITSCRR271 pKa = 11.84YY272 pKa = 9.99DD273 pKa = 3.51RR274 pKa = 11.84GTMNCNCDD282 pKa = 3.15RR283 pKa = 11.84VISTKK288 pKa = 10.64LLAIRR293 pKa = 11.84GSFLTPYY300 pKa = 10.09IEE302 pKa = 4.25WDD304 pKa = 3.67QVVHH308 pKa = 7.82DD309 pKa = 4.53ITTDD313 pKa = 3.11KK314 pKa = 10.87QSPYY318 pKa = 11.01DD319 pKa = 3.73LPTT322 pKa = 3.53

Molecular weight: 37.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

4185

113

2038

597.9

68.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.349 ± 0.48

1.983 ± 0.286

6.523 ± 0.296

6.786 ± 0.438

3.297 ± 0.568

4.851 ± 0.121

2.7 ± 0.17

8.363 ± 0.29

8.005 ± 0.213

8.196 ± 1.082

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.465 ± 0.2

4.683 ± 0.313

3.56 ± 0.482

2.413 ± 0.346

5.018 ± 0.26

8.984 ± 0.355

5.926 ± 0.527

5.52 ± 0.685

1.338 ± 0.188

4.038 ± 0.421
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski