Ruthia magnifica subsp. Calyptogena magnifica

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Gammaproteobacteria incertae sedis; sulfur-oxidizing symbionts; Candidatus Ruthia; Candidatus Ruthia magnifica

Average proteome isoelectric point is 7.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 976 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A1AXI4|A1AXI4_RUTMC ATP-dependent DNA helicase RecG OS=Ruthia magnifica subsp. Calyptogena magnifica OX=413404 GN=Rmag_0928 PE=3 SV=1
MM1 pKa = 8.33SEE3 pKa = 3.83IRR5 pKa = 11.84DD6 pKa = 3.7DD7 pKa = 3.65RR8 pKa = 11.84QYY10 pKa = 10.48TQTHH14 pKa = 5.41EE15 pKa = 4.41WILDD19 pKa = 3.81HH20 pKa = 7.65GDD22 pKa = 3.07GTYY25 pKa = 11.09TMGITDD31 pKa = 4.32HH32 pKa = 6.77AQALLGDD39 pKa = 4.04MVFVEE44 pKa = 5.35LPNIDD49 pKa = 4.48DD50 pKa = 3.8EE51 pKa = 5.21VSIEE55 pKa = 4.55DD56 pKa = 3.7EE57 pKa = 4.41FCVVEE62 pKa = 4.33SVKK65 pKa = 10.7AASGVYY71 pKa = 10.17APADD75 pKa = 3.49LQIIKK80 pKa = 10.25VNKK83 pKa = 10.23ALDD86 pKa = 4.18DD87 pKa = 4.08EE88 pKa = 5.17PEE90 pKa = 4.8LINYY94 pKa = 8.46SCYY97 pKa = 10.6DD98 pKa = 3.87DD99 pKa = 3.56GWLVKK104 pKa = 10.33FKK106 pKa = 10.77SHH108 pKa = 7.05SVDD111 pKa = 3.29DD112 pKa = 4.25LMNVAVYY119 pKa = 10.15AQILDD124 pKa = 3.6

Molecular weight: 13.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A1AXT8|A1AXT8_RUTMC TauD domain-containing protein OS=Ruthia magnifica subsp. Calyptogena magnifica OX=413404 GN=Rmag_1041 PE=4 SV=1
MM1 pKa = 6.65KK2 pKa = 10.14QKK4 pKa = 9.65RR5 pKa = 11.84TFQPSVIKK13 pKa = 10.52RR14 pKa = 11.84KK15 pKa = 8.13RR16 pKa = 11.84THH18 pKa = 6.03GFRR21 pKa = 11.84SRR23 pKa = 11.84MSTKK27 pKa = 10.23SGRR30 pKa = 11.84AVINARR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.5KK39 pKa = 9.79GRR41 pKa = 11.84KK42 pKa = 8.85RR43 pKa = 11.84LAAA46 pKa = 4.42

Molecular weight: 5.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

976

0

976

305790

37

2819

313.3

35.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.751 ± 0.077

1.031 ± 0.03

5.495 ± 0.055

5.291 ± 0.057

4.406 ± 0.055

6.434 ± 0.074

2.169 ± 0.038

9.004 ± 0.07

7.47 ± 0.08

10.036 ± 0.096

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.652 ± 0.043

5.682 ± 0.06

3.14 ± 0.037

4.174 ± 0.057

3.634 ± 0.046

6.619 ± 0.059

5.423 ± 0.08

6.583 ± 0.062

0.897 ± 0.023

3.109 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski