Geobacillus virus E2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A6M9A8|A6M9A8_9CAUD Uncharacterized protein OS=Geobacillus virus E2 OX=447909 PE=4 SV=1
MM1 pKa = 7.78FIGLGLGVGTVILIQLIVDD20 pKa = 4.16LFKK23 pKa = 11.56DD24 pKa = 3.41EE25 pKa = 4.32FTDD28 pKa = 3.95FRR30 pKa = 11.84WYY32 pKa = 10.62LICTCIMLLCFALDD46 pKa = 3.98VVWGG50 pKa = 3.86

Molecular weight: 5.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A6M9A7|A6M9A7_9CAUD Uncharacterized protein OS=Geobacillus virus E2 OX=447909 PE=4 SV=1
MM1 pKa = 7.72LFTSNKK7 pKa = 8.2NATPFGVTLFGLQRR21 pKa = 11.84DD22 pKa = 3.77RR23 pKa = 11.84QRR25 pKa = 11.84IYY27 pKa = 9.33EE28 pKa = 4.14KK29 pKa = 10.59EE30 pKa = 3.73RR31 pKa = 11.84SRR33 pKa = 11.84CAGYY37 pKa = 10.71EE38 pKa = 3.72RR39 pKa = 11.84TTRR42 pKa = 11.84TTIKK46 pKa = 10.71LMDD49 pKa = 4.11SDD51 pKa = 4.64FPPGIFAINFIPCPITFQLPAATRR75 pKa = 11.84ARR77 pKa = 11.84HH78 pKa = 6.11LFQFMPRR85 pKa = 11.84LQCLGVKK92 pKa = 9.21TIIACWRR99 pKa = 11.84CEE101 pKa = 3.77FF102 pKa = 4.69

Molecular weight: 11.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

62

0

62

12807

44

1415

206.6

23.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.605 ± 0.684

0.953 ± 0.171

5.739 ± 0.376

7.957 ± 0.485

4.349 ± 0.318

6.059 ± 0.315

1.382 ± 0.151

6.934 ± 0.269

7.754 ± 0.392

8.347 ± 0.459

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.624 ± 0.213

4.989 ± 0.51

3.1 ± 0.246

4.17 ± 0.29

5.364 ± 0.372

5.349 ± 0.4

5.747 ± 0.27

6.169 ± 0.255

1.366 ± 0.101

4.045 ± 0.325
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski