Azoarcus sp. (strain BH72)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Rhodocyclales; Zoogloeaceae; Azoarcus

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3981 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A1K2R6|A1K2R6_AZOSB RNA polymerase sigma-54 factor OS=Azoarcus sp. (strain BH72) OX=418699 GN=rpoN1 PE=3 SV=1
MM1 pKa = 7.95EE2 pKa = 4.6IRR4 pKa = 11.84KK5 pKa = 9.08VVLGCATACCAIASPSVSAALLPLPDD31 pKa = 3.73TVIDD35 pKa = 4.13GVVVSIQYY43 pKa = 11.25GDD45 pKa = 4.12FYY47 pKa = 11.33SYY49 pKa = 8.95STRR52 pKa = 11.84VLDD55 pKa = 4.31YY56 pKa = 10.13LQPDD60 pKa = 3.92AGWDD64 pKa = 3.42KK65 pKa = 11.4SAGTGTLDD73 pKa = 3.96VIITTRR79 pKa = 11.84SAGQSNSNGGLAPYY93 pKa = 9.36NIPDD97 pKa = 5.58PITNVNEE104 pKa = 4.11NPTSGSWGMGGTSATTMLVSDD125 pKa = 5.02LYY127 pKa = 10.97RR128 pKa = 11.84YY129 pKa = 9.67LQDD132 pKa = 3.8TFDD135 pKa = 3.17ATIPVFTFDD144 pKa = 4.14QNEE147 pKa = 4.4TGGNPSLFAMAKK159 pKa = 10.1VEE161 pKa = 4.91IFDD164 pKa = 3.99KK165 pKa = 11.06DD166 pKa = 3.63GNLLEE171 pKa = 4.92DD172 pKa = 4.67WILGDD177 pKa = 3.54GVNPVEE183 pKa = 5.01APGTVCVDD191 pKa = 3.65PDD193 pKa = 4.29SLPLKK198 pKa = 10.21CFSNNVGSGAFDD210 pKa = 4.19YY211 pKa = 11.11ILFAPTMDD219 pKa = 3.33LTNYY223 pKa = 9.18NAEE226 pKa = 3.99GNIFKK231 pKa = 10.88VSWTFSSVDD240 pKa = 3.58DD241 pKa = 4.51GGEE244 pKa = 3.87EE245 pKa = 3.83ATLTGRR251 pKa = 11.84FTGSICVEE259 pKa = 4.13NPSAPQCQVIPEE271 pKa = 4.49PGALALIGGGLLALFALRR289 pKa = 11.84RR290 pKa = 11.84RR291 pKa = 11.84WGAA294 pKa = 2.95

Molecular weight: 31.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A1K2C6|A1K2C6_AZOSB Conserved hypothetical membrane protein OS=Azoarcus sp. (strain BH72) OX=418699 GN=azo0364 PE=4 SV=1
MM1 pKa = 7.33MSRR4 pKa = 11.84IKK6 pKa = 10.91GRR8 pKa = 11.84DD9 pKa = 3.19TGPEE13 pKa = 3.35LSLRR17 pKa = 11.84RR18 pKa = 11.84NIWALGLRR26 pKa = 11.84YY27 pKa = 9.58RR28 pKa = 11.84LQYY31 pKa = 10.47RR32 pKa = 11.84IGRR35 pKa = 11.84TRR37 pKa = 11.84PDD39 pKa = 3.19MVFVRR44 pKa = 11.84ARR46 pKa = 11.84LAVFVDD52 pKa = 4.01GCFWHH57 pKa = 7.06GCPQHH62 pKa = 6.05STMPKK67 pKa = 8.63NNRR70 pKa = 11.84DD71 pKa = 2.89FWEE74 pKa = 4.16RR75 pKa = 11.84KK76 pKa = 8.1LRR78 pKa = 11.84RR79 pKa = 11.84NRR81 pKa = 11.84EE82 pKa = 3.3RR83 pKa = 11.84DD84 pKa = 3.25AEE86 pKa = 4.28NTHH89 pKa = 5.76SLEE92 pKa = 4.44AEE94 pKa = 4.14GWRR97 pKa = 11.84VLRR100 pKa = 11.84LWEE103 pKa = 4.35HH104 pKa = 7.28EE105 pKa = 4.25IQASPADD112 pKa = 3.82CARR115 pKa = 11.84RR116 pKa = 11.84IAVMLGKK123 pKa = 10.31AEE125 pKa = 3.98EE126 pKa = 4.47SKK128 pKa = 11.31AA129 pKa = 3.53

Molecular weight: 15.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3981

0

3981

1326302

31

2109

333.2

36.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.574 ± 0.066

0.97 ± 0.015

5.492 ± 0.027

5.784 ± 0.036

3.547 ± 0.025

8.511 ± 0.029

2.272 ± 0.019

4.266 ± 0.026

2.737 ± 0.031

11.048 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.185 ± 0.018

2.412 ± 0.022

5.339 ± 0.031

3.326 ± 0.023

7.788 ± 0.04

4.759 ± 0.027

4.735 ± 0.032

7.603 ± 0.034

1.387 ± 0.014

2.265 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski