Bohle iridovirus

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes; Pimascovirales; Iridoviridae; Alphairidovirinae; Ranavirus; Frog virus 3

Average proteome isoelectric point is 7.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 100 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A192GQ78|A0A192GQ78_FRG3V Putative SAP domain-containing protein OS=Bohle iridovirus OX=100220 GN=ORF51 PE=4 SV=1
MM1 pKa = 7.69EE2 pKa = 5.22EE3 pKa = 3.95VDD5 pKa = 5.03DD6 pKa = 4.3CAGLNSEE13 pKa = 5.08AGALTAGALTAGALTAGALTAGALTAGALTAGALTAGALTAGEE56 pKa = 4.35LLEE59 pKa = 4.46HH60 pKa = 6.99

Molecular weight: 5.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A192GPY1|A0A192GPY1_FRG3V Uncharacterized protein OS=Bohle iridovirus OX=100220 GN=ORF60 PE=4 SV=1
MM1 pKa = 7.11YY2 pKa = 10.24SVRR5 pKa = 11.84NSGCSVGCSPRR16 pKa = 11.84QGASPIMFGPSLGAMLSAPVVRR38 pKa = 11.84ASSPVVKK45 pKa = 10.55ASSPVVKK52 pKa = 10.3RR53 pKa = 11.84KK54 pKa = 10.35SLVKK58 pKa = 10.35RR59 pKa = 11.84KK60 pKa = 10.3SPVKK64 pKa = 10.27KK65 pKa = 10.51SPLKK69 pKa = 10.31KK70 pKa = 9.97RR71 pKa = 11.84SPLRR75 pKa = 11.84TSPVKK80 pKa = 10.44RR81 pKa = 11.84KK82 pKa = 10.42SPVKK86 pKa = 10.07RR87 pKa = 11.84KK88 pKa = 10.4SPVKK92 pKa = 10.07RR93 pKa = 11.84KK94 pKa = 10.4SPVKK98 pKa = 10.07RR99 pKa = 11.84KK100 pKa = 10.4SPVKK104 pKa = 10.07RR105 pKa = 11.84KK106 pKa = 10.4SPVKK110 pKa = 10.07RR111 pKa = 11.84KK112 pKa = 10.4SPVKK116 pKa = 10.07RR117 pKa = 11.84KK118 pKa = 10.4SPVKK122 pKa = 10.07RR123 pKa = 11.84KK124 pKa = 10.4SPVKK128 pKa = 10.23RR129 pKa = 11.84KK130 pKa = 10.33SPVKK134 pKa = 10.02KK135 pKa = 10.07RR136 pKa = 11.84SPVKK140 pKa = 10.15RR141 pKa = 11.84KK142 pKa = 9.96SPAKK146 pKa = 10.0RR147 pKa = 11.84RR148 pKa = 11.84QQVCFMATKK157 pKa = 10.43

Molecular weight: 17.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

100

0

100

29189

34

1293

291.9

32.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.027 ± 0.256

2.128 ± 0.139

6.002 ± 0.162

5.543 ± 0.21

3.279 ± 0.111

7.376 ± 0.269

2.145 ± 0.155

3.299 ± 0.129

5.646 ± 0.26

8.119 ± 0.297

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.967 ± 0.129

2.628 ± 0.125

6.256 ± 0.284

2.83 ± 0.174

7.232 ± 0.326

7.352 ± 0.246

5.447 ± 0.193

8.328 ± 0.251

1.401 ± 0.103

2.994 ± 0.128
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski