Amino acid dipepetide frequency for Halonotius sp. F13-13

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.517AlaAla: 15.517 ± 0.22
0.668AlaCys: 0.668 ± 0.029
11.404AlaAsp: 11.404 ± 0.162
8.849AlaGlu: 8.849 ± 0.112
3.686AlaPhe: 3.686 ± 0.078
9.452AlaGly: 9.452 ± 0.134
1.77AlaHis: 1.77 ± 0.051
5.796AlaIle: 5.796 ± 0.088
2.064AlaLys: 2.064 ± 0.052
9.107AlaLeu: 9.107 ± 0.126
2.151AlaMet: 2.151 ± 0.059
2.488AlaAsn: 2.488 ± 0.063
3.681AlaPro: 3.681 ± 0.072
2.213AlaGln: 2.213 ± 0.06
5.117AlaArg: 5.117 ± 0.101
5.326AlaSer: 5.326 ± 0.093
8.203AlaThr: 8.203 ± 0.129
10.704AlaVal: 10.704 ± 0.146
1.018AlaTrp: 1.018 ± 0.033
2.621AlaTyr: 2.621 ± 0.065
0.0AlaXaa: 0.0 ± 0.0
Cys
0.573CysAla: 0.573 ± 0.028
0.093CysCys: 0.093 ± 0.013
0.558CysAsp: 0.558 ± 0.029
0.655CysGlu: 0.655 ± 0.033
0.226CysPhe: 0.226 ± 0.019
0.778CysGly: 0.778 ± 0.035
0.19CysHis: 0.19 ± 0.015
0.238CysIle: 0.238 ± 0.02
0.124CysLys: 0.124 ± 0.012
0.547CysLeu: 0.547 ± 0.03
0.103CysMet: 0.103 ± 0.013
0.185CysAsn: 0.185 ± 0.017
0.485CysPro: 0.485 ± 0.028
0.188CysGln: 0.188 ± 0.016
0.477CysArg: 0.477 ± 0.027
0.449CysSer: 0.449 ± 0.028
0.374CysThr: 0.374 ± 0.026
0.471CysVal: 0.471 ± 0.028
0.093CysTrp: 0.093 ± 0.011
0.191CysTyr: 0.191 ± 0.017
0.0CysXaa: 0.0 ± 0.0
Asp
10.75AspAla: 10.75 ± 0.166
0.761AspCys: 0.761 ± 0.039
9.669AspAsp: 9.669 ± 0.16
7.694AspGlu: 7.694 ± 0.123
2.05AspPhe: 2.05 ± 0.062
7.923AspGly: 7.923 ± 0.118
2.042AspHis: 2.042 ± 0.048
4.06AspIle: 4.06 ± 0.082
1.234AspLys: 1.234 ± 0.042
6.601AspLeu: 6.601 ± 0.117
1.211AspMet: 1.211 ± 0.043
2.043AspAsn: 2.043 ± 0.064
4.53AspPro: 4.53 ± 0.093
2.05AspGln: 2.05 ± 0.052
6.588AspArg: 6.588 ± 0.128
4.884AspSer: 4.884 ± 0.089
5.487AspThr: 5.487 ± 0.087
6.057AspVal: 6.057 ± 0.094
0.893AspTrp: 0.893 ± 0.037
1.941AspTyr: 1.941 ± 0.051
0.0AspXaa: 0.0 ± 0.0
Glu
8.878GluAla: 8.878 ± 0.129
0.488GluCys: 0.488 ± 0.029
4.618GluAsp: 4.618 ± 0.097
6.142GluGlu: 6.142 ± 0.131
2.785GluPhe: 2.785 ± 0.066
4.707GluGly: 4.707 ± 0.091
1.813GluHis: 1.813 ± 0.05
4.742GluIle: 4.742 ± 0.092
1.784GluLys: 1.784 ± 0.049
8.336GluLeu: 8.336 ± 0.121
1.814GluMet: 1.814 ± 0.051
2.001GluAsn: 2.001 ± 0.053
4.34GluPro: 4.34 ± 0.091
3.289GluGln: 3.289 ± 0.075
5.461GluArg: 5.461 ± 0.087
5.11GluSer: 5.11 ± 0.094
7.217GluThr: 7.217 ± 0.106
5.004GluVal: 5.004 ± 0.095
1.053GluTrp: 1.053 ± 0.039
2.471GluTyr: 2.471 ± 0.066
0.0GluXaa: 0.0 ± 0.0
Phe
3.347PheAla: 3.347 ± 0.066
0.264PheCys: 0.264 ± 0.019
3.149PheAsp: 3.149 ± 0.075
3.148PheGlu: 3.148 ± 0.074
0.976PhePhe: 0.976 ± 0.042
3.16PheGly: 3.16 ± 0.081
0.579PheHis: 0.579 ± 0.029
1.378PheIle: 1.378 ± 0.051
0.527PheLys: 0.527 ± 0.025
2.565PheLeu: 2.565 ± 0.079
0.49PheMet: 0.49 ± 0.029
0.742PheAsn: 0.742 ± 0.034
1.211PhePro: 1.211 ± 0.032
0.826PheGln: 0.826 ± 0.035
1.65PheArg: 1.65 ± 0.048
1.71PheSer: 1.71 ± 0.048
1.918PheThr: 1.918 ± 0.049
2.918PheVal: 2.918 ± 0.063
0.37PheTrp: 0.37 ± 0.023
0.849PheTyr: 0.849 ± 0.034
0.0PheXaa: 0.0 ± 0.0
Gly
7.072GlyAla: 7.072 ± 0.108
0.668GlyCys: 0.668 ± 0.033
6.923GlyAsp: 6.923 ± 0.104
6.484GlyGlu: 6.484 ± 0.095
2.985GlyPhe: 2.985 ± 0.072
7.286GlyGly: 7.286 ± 0.152
1.639GlyHis: 1.639 ± 0.052
4.622GlyIle: 4.622 ± 0.096
1.799GlyLys: 1.799 ± 0.064
7.322GlyLeu: 7.322 ± 0.133
1.572GlyMet: 1.572 ± 0.051
1.855GlyAsn: 1.855 ± 0.057
2.826GlyPro: 2.826 ± 0.072
2.28GlyGln: 2.28 ± 0.052
4.403GlyArg: 4.403 ± 0.081
4.87GlySer: 4.87 ± 0.091
5.322GlyThr: 5.322 ± 0.089
7.268GlyVal: 7.268 ± 0.113
1.032GlyTrp: 1.032 ± 0.042
2.59GlyTyr: 2.59 ± 0.058
0.0GlyXaa: 0.0 ± 0.0
His
1.993HisAla: 1.993 ± 0.059
0.174HisCys: 0.174 ± 0.015
1.819HisAsp: 1.819 ± 0.063
1.551HisGlu: 1.551 ± 0.047
0.477HisPhe: 0.477 ± 0.027
1.813HisGly: 1.813 ± 0.056
0.576HisHis: 0.576 ± 0.034
0.922HisIle: 0.922 ± 0.039
0.343HisLys: 0.343 ± 0.021
1.644HisLeu: 1.644 ± 0.048
0.25HisMet: 0.25 ± 0.017
0.601HisAsn: 0.601 ± 0.027
1.159HisPro: 1.159 ± 0.04
0.552HisGln: 0.552 ± 0.028
1.271HisArg: 1.271 ± 0.044
0.901HisSer: 0.901 ± 0.038
1.218HisThr: 1.218 ± 0.048
1.555HisVal: 1.555 ± 0.045
0.208HisTrp: 0.208 ± 0.017
0.533HisTyr: 0.533 ± 0.023
0.0HisXaa: 0.0 ± 0.0
Ile
5.655IleAla: 5.655 ± 0.098
0.326IleCys: 0.326 ± 0.021
5.406IleAsp: 5.406 ± 0.102
5.203IleGlu: 5.203 ± 0.092
1.153IlePhe: 1.153 ± 0.044
4.325IleGly: 4.325 ± 0.092
1.018IleHis: 1.018 ± 0.034
2.038IleIle: 2.038 ± 0.058
1.014IleLys: 1.014 ± 0.039
3.329IleLeu: 3.329 ± 0.08
0.57IleMet: 0.57 ± 0.032
1.388IleAsn: 1.388 ± 0.044
2.294IlePro: 2.294 ± 0.063
1.384IleGln: 1.384 ± 0.041
2.654IleArg: 2.654 ± 0.068
2.512IleSer: 2.512 ± 0.055
3.323IleThr: 3.323 ± 0.078
4.025IleVal: 4.025 ± 0.095
0.333IleTrp: 0.333 ± 0.022
1.059IleTyr: 1.059 ± 0.041
0.0IleXaa: 0.0 ± 0.0
Lys
1.86LysAla: 1.86 ± 0.058
0.1LysCys: 0.1 ± 0.012
1.07LysAsp: 1.07 ± 0.041
1.446LysGlu: 1.446 ± 0.049
0.519LysPhe: 0.519 ± 0.026
1.287LysGly: 1.287 ± 0.045
0.478LysHis: 0.478 ± 0.027
0.948LysIle: 0.948 ± 0.039
0.53LysLys: 0.53 ± 0.034
1.866LysLeu: 1.866 ± 0.058
0.363LysMet: 0.363 ± 0.025
0.57LysAsn: 0.57 ± 0.033
1.0LysPro: 1.0 ± 0.034
1.049LysGln: 1.049 ± 0.038
1.604LysArg: 1.604 ± 0.051
1.241LysSer: 1.241 ± 0.043
1.555LysThr: 1.555 ± 0.056
1.078LysVal: 1.078 ± 0.041
0.244LysTrp: 0.244 ± 0.02
0.559LysTyr: 0.559 ± 0.028
0.0LysXaa: 0.0 ± 0.0
Leu
10.605LeuAla: 10.605 ± 0.145
0.556LeuCys: 0.556 ± 0.028
7.527LeuAsp: 7.527 ± 0.102
5.852LeuGlu: 5.852 ± 0.111
3.045LeuPhe: 3.045 ± 0.075
7.135LeuGly: 7.135 ± 0.134
1.469LeuHis: 1.469 ± 0.043
3.766LeuIle: 3.766 ± 0.081
1.718LeuLys: 1.718 ± 0.052
8.856LeuLeu: 8.856 ± 0.158
1.378LeuMet: 1.378 ± 0.042
1.996LeuAsn: 1.996 ± 0.058
4.215LeuPro: 4.215 ± 0.089
2.535LeuGln: 2.535 ± 0.063
5.304LeuArg: 5.304 ± 0.097
5.837LeuSer: 5.837 ± 0.098
6.223LeuThr: 6.223 ± 0.104
7.319LeuVal: 7.319 ± 0.119
0.883LeuTrp: 0.883 ± 0.04
2.187LeuTyr: 2.187 ± 0.061
0.0LeuXaa: 0.0 ± 0.0
Met
1.891MetAla: 1.891 ± 0.056
0.116MetCys: 0.116 ± 0.013
1.184MetAsp: 1.184 ± 0.047
1.183MetGlu: 1.183 ± 0.041
0.434MetPhe: 0.434 ± 0.024
1.357MetGly: 1.357 ± 0.05
0.343MetHis: 0.343 ± 0.022
0.76MetIle: 0.76 ± 0.03
0.402MetLys: 0.402 ± 0.024
1.524MetLeu: 1.524 ± 0.042
0.293MetMet: 0.293 ± 0.019
0.573MetAsn: 0.573 ± 0.026
0.771MetPro: 0.771 ± 0.031
0.552MetGln: 0.552 ± 0.029
0.94MetArg: 0.94 ± 0.033
1.513MetSer: 1.513 ± 0.046
1.483MetThr: 1.483 ± 0.042
1.247MetVal: 1.247 ± 0.044
0.127MetTrp: 0.127 ± 0.015
0.335MetTyr: 0.335 ± 0.021
0.0MetXaa: 0.0 ± 0.0
Asn
2.742AsnAla: 2.742 ± 0.063
0.209AsnCys: 0.209 ± 0.016
1.933AsnAsp: 1.933 ± 0.054
1.979AsnGlu: 1.979 ± 0.054
0.714AsnPhe: 0.714 ± 0.034
1.976AsnGly: 1.976 ± 0.055
0.477AsnHis: 0.477 ± 0.025
1.1AsnIle: 1.1 ± 0.041
0.544AsnLys: 0.544 ± 0.032
2.015AsnLeu: 2.015 ± 0.051
0.476AsnMet: 0.476 ± 0.027
0.669AsnAsn: 0.669 ± 0.035
1.611AsnPro: 1.611 ± 0.048
0.707AsnGln: 0.707 ± 0.034
1.724AsnArg: 1.724 ± 0.043
1.199AsnSer: 1.199 ± 0.046
1.612AsnThr: 1.612 ± 0.048
2.035AsnVal: 2.035 ± 0.056
0.286AsnTrp: 0.286 ± 0.021
0.711AsnTyr: 0.711 ± 0.033
0.0AsnXaa: 0.0 ± 0.0
Pro
5.12ProAla: 5.12 ± 0.088
0.258ProCys: 0.258 ± 0.019
4.851ProAsp: 4.851 ± 0.089
4.368ProGlu: 4.368 ± 0.076
1.563ProPhe: 1.563 ± 0.052
3.392ProGly: 3.392 ± 0.062
0.801ProHis: 0.801 ± 0.032
2.229ProIle: 2.229 ± 0.053
0.823ProLys: 0.823 ± 0.033
3.646ProLeu: 3.646 ± 0.075
0.796ProMet: 0.796 ± 0.029
1.174ProAsn: 1.174 ± 0.045
2.275ProPro: 2.275 ± 0.063
1.254ProGln: 1.254 ± 0.048
1.987ProArg: 1.987 ± 0.056
2.496ProSer: 2.496 ± 0.069
3.739ProThr: 3.739 ± 0.077
3.862ProVal: 3.862 ± 0.072
0.46ProTrp: 0.46 ± 0.025
1.014ProTyr: 1.014 ± 0.043
0.0ProXaa: 0.0 ± 0.0
Gln
3.141GlnAla: 3.141 ± 0.079
0.201GlnCys: 0.201 ± 0.018
1.39GlnAsp: 1.39 ± 0.05
1.897GlnGlu: 1.897 ± 0.057
1.184GlnPhe: 1.184 ± 0.037
1.642GlnGly: 1.642 ± 0.048
0.658GlnHis: 0.658 ± 0.029
1.436GlnIle: 1.436 ± 0.045
0.654GlnLys: 0.654 ± 0.03
3.106GlnLeu: 3.106 ± 0.076
0.615GlnMet: 0.615 ± 0.029
0.759GlnAsn: 0.759 ± 0.032
1.584GlnPro: 1.584 ± 0.053
1.628GlnGln: 1.628 ± 0.051
2.252GlnArg: 2.252 ± 0.063
1.833GlnSer: 1.833 ± 0.058
2.022GlnThr: 2.022 ± 0.058
1.902GlnVal: 1.902 ± 0.05
0.389GlnTrp: 0.389 ± 0.026
0.912GlnTyr: 0.912 ± 0.037
0.0GlnXaa: 0.0 ± 0.0
Arg
5.633ArgAla: 5.633 ± 0.102
0.42ArgCys: 0.42 ± 0.026
4.333ArgAsp: 4.333 ± 0.092
5.745ArgGlu: 5.745 ± 0.106
2.166ArgPhe: 2.166 ± 0.059
3.884ArgGly: 3.884 ± 0.073
1.138ArgHis: 1.138 ± 0.038
3.021ArgIle: 3.021 ± 0.077
1.389ArgLys: 1.389 ± 0.046
5.892ArgLeu: 5.892 ± 0.101
1.068ArgMet: 1.068 ± 0.039
1.629ArgAsn: 1.629 ± 0.048
2.273ArgPro: 2.273 ± 0.066
2.16ArgGln: 2.16 ± 0.056
3.986ArgArg: 3.986 ± 0.095
3.174ArgSer: 3.174 ± 0.064
3.365ArgThr: 3.365 ± 0.075
4.647ArgVal: 4.647 ± 0.086
0.685ArgTrp: 0.685 ± 0.032
1.735ArgTyr: 1.735 ± 0.053
0.0ArgXaa: 0.0 ± 0.0
Ser
5.216SerAla: 5.216 ± 0.083
0.303SerCys: 0.303 ± 0.019
4.74SerAsp: 4.74 ± 0.091
4.59SerGlu: 4.59 ± 0.098
1.805SerPhe: 1.805 ± 0.048
4.779SerGly: 4.779 ± 0.08
1.096SerHis: 1.096 ± 0.041
2.798SerIle: 2.798 ± 0.064
1.204SerLys: 1.204 ± 0.044
5.191SerLeu: 5.191 ± 0.082
1.092SerMet: 1.092 ± 0.046
1.333SerAsn: 1.333 ± 0.045
2.711SerPro: 2.711 ± 0.076
1.618SerGln: 1.618 ± 0.049
2.999SerArg: 2.999 ± 0.07
2.947SerSer: 2.947 ± 0.082
4.061SerThr: 4.061 ± 0.087
4.964SerVal: 4.964 ± 0.09
0.555SerTrp: 0.555 ± 0.028
1.485SerTyr: 1.485 ± 0.049
0.0SerXaa: 0.0 ± 0.0
Thr
8.526ThrAla: 8.526 ± 0.141
0.388ThrCys: 0.388 ± 0.026
6.736ThrAsp: 6.736 ± 0.115
5.268ThrGlu: 5.268 ± 0.099
1.971ThrPhe: 1.971 ± 0.055
5.784ThrGly: 5.784 ± 0.104
1.339ThrHis: 1.339 ± 0.045
3.687ThrIle: 3.687 ± 0.073
1.169ThrLys: 1.169 ± 0.037
6.301ThrLeu: 6.301 ± 0.096
1.039ThrMet: 1.039 ± 0.042
1.803ThrAsn: 1.803 ± 0.049
3.805ThrPro: 3.805 ± 0.089
1.883ThrGln: 1.883 ± 0.051
3.159ThrArg: 3.159 ± 0.076
2.796ThrSer: 2.796 ± 0.08
5.611ThrThr: 5.611 ± 0.119
7.341ThrVal: 7.341 ± 0.106
0.6ThrTrp: 0.6 ± 0.025
1.732ThrTyr: 1.732 ± 0.059
0.0ThrXaa: 0.0 ± 0.0
Val
9.838ValAla: 9.838 ± 0.148
0.65ValCys: 0.65 ± 0.03
8.004ValAsp: 8.004 ± 0.113
6.77ValGlu: 6.77 ± 0.115
2.855ValPhe: 2.855 ± 0.074
7.302ValGly: 7.302 ± 0.126
1.328ValHis: 1.328 ± 0.043
4.005ValIle: 4.005 ± 0.083
1.34ValLys: 1.34 ± 0.044
6.844ValLeu: 6.844 ± 0.137
1.222ValMet: 1.222 ± 0.039
1.894ValAsn: 1.894 ± 0.055
3.65ValPro: 3.65 ± 0.072
1.77ValGln: 1.77 ± 0.05
4.25ValArg: 4.25 ± 0.081
4.959ValSer: 4.959 ± 0.087
5.911ValThr: 5.911 ± 0.107
8.323ValVal: 8.323 ± 0.12
0.714ValTrp: 0.714 ± 0.032
1.893ValTyr: 1.893 ± 0.05
0.0ValXaa: 0.0 ± 0.0
Trp
0.918TrpAla: 0.918 ± 0.034
0.095TrpCys: 0.095 ± 0.01
0.657TrpAsp: 0.657 ± 0.03
0.852TrpGlu: 0.852 ± 0.035
0.396TrpPhe: 0.396 ± 0.029
0.731TrpGly: 0.731 ± 0.036
0.215TrpHis: 0.215 ± 0.016
0.491TrpIle: 0.491 ± 0.029
0.234TrpLys: 0.234 ± 0.018
1.218TrpLeu: 1.218 ± 0.044
0.163TrpMet: 0.163 ± 0.015
0.335TrpAsn: 0.335 ± 0.022
0.421TrpPro: 0.421 ± 0.025
0.438TrpGln: 0.438 ± 0.026
0.778TrpArg: 0.778 ± 0.034
0.462TrpSer: 0.462 ± 0.031
0.653TrpThr: 0.653 ± 0.033
0.809TrpVal: 0.809 ± 0.04
0.165TrpTrp: 0.165 ± 0.016
0.379TrpTyr: 0.379 ± 0.024
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.561TyrAla: 2.561 ± 0.064
0.262TyrCys: 0.262 ± 0.018
2.498TyrAsp: 2.498 ± 0.069
2.379TyrGlu: 2.379 ± 0.071
0.785TyrPhe: 0.785 ± 0.036
2.103TyrGly: 2.103 ± 0.054
0.591TyrHis: 0.591 ± 0.026
0.972TyrIle: 0.972 ± 0.037
0.473TyrLys: 0.473 ± 0.026
2.495TyrLeu: 2.495 ± 0.068
0.361TyrMet: 0.361 ± 0.024
0.681TyrAsn: 0.681 ± 0.032
1.266TyrPro: 1.266 ± 0.044
0.83TyrGln: 0.83 ± 0.036
1.741TyrArg: 1.741 ± 0.046
1.266TyrSer: 1.266 ± 0.044
1.64TyrThr: 1.64 ± 0.055
1.992TyrVal: 1.992 ± 0.049
0.301TyrTrp: 0.301 ± 0.023
0.755TyrTyr: 0.755 ± 0.033
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2460 proteins (717023 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski