Eubacterium plexicaudatum ASF492

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae; Eubacterium; Eubacterium plexicaudatum

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6154 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|N2A476|N2A476_9FIRM Uncharacterized protein (Fragment) OS=Eubacterium plexicaudatum ASF492 OX=1235802 GN=C823_04627 PE=4 SV=1
MM1 pKa = 7.5KK2 pKa = 10.4KK3 pKa = 10.32KK4 pKa = 10.22ILAVLLAGMMAFSMAACGSANDD26 pKa = 4.67GNADD30 pKa = 3.8SNKK33 pKa = 10.19EE34 pKa = 3.9NTDD37 pKa = 2.95NSGADD42 pKa = 3.3GSEE45 pKa = 4.04ADD47 pKa = 5.1DD48 pKa = 4.19SKK50 pKa = 11.99SDD52 pKa = 4.09DD53 pKa = 4.46GEE55 pKa = 4.42SSDD58 pKa = 5.83DD59 pKa = 3.97GSNDD63 pKa = 3.16DD64 pKa = 4.44SAPASDD70 pKa = 4.44MNIAMITDD78 pKa = 3.58SGDD81 pKa = 3.07ITDD84 pKa = 3.6QSFNQTTYY92 pKa = 8.47EE93 pKa = 4.04TSKK96 pKa = 10.97AWAEE100 pKa = 4.04ANNVPFTYY108 pKa = 10.25YY109 pKa = 10.72KK110 pKa = 10.04PDD112 pKa = 3.54SDD114 pKa = 4.46SDD116 pKa = 3.6EE117 pKa = 4.14ARR119 pKa = 11.84NASVDD124 pKa = 3.46QAVAGGANVLMMPGYY139 pKa = 9.11MFAASVVAKK148 pKa = 10.8AEE150 pKa = 4.03MYY152 pKa = 10.54PDD154 pKa = 3.46VKK156 pKa = 10.84FIALDD161 pKa = 3.48VGAGDD166 pKa = 4.39LLEE169 pKa = 5.14KK170 pKa = 10.8GVGEE174 pKa = 4.54GYY176 pKa = 10.67DD177 pKa = 4.08YY178 pKa = 11.58NPEE181 pKa = 3.97NYY183 pKa = 9.51DD184 pKa = 3.3VKK186 pKa = 10.76EE187 pKa = 4.13YY188 pKa = 10.55YY189 pKa = 10.2HH190 pKa = 7.3ADD192 pKa = 3.08NVYY195 pKa = 9.0CCTYY199 pKa = 10.33QEE201 pKa = 4.24EE202 pKa = 4.68LSGYY206 pKa = 7.19MAGYY210 pKa = 9.98AAVKK214 pKa = 10.23LGYY217 pKa = 9.73KK218 pKa = 10.17HH219 pKa = 6.66LGFLGGMSVPAVTRR233 pKa = 11.84FGYY236 pKa = 10.66GYY238 pKa = 10.48LQGADD243 pKa = 3.32AAAKK247 pKa = 9.43EE248 pKa = 4.03LGIEE252 pKa = 4.3GEE254 pKa = 4.29VEE256 pKa = 3.67AEE258 pKa = 4.33YY259 pKa = 11.24VCGGQFYY266 pKa = 11.29GDD268 pKa = 3.9ADD270 pKa = 3.24ITAAMDD276 pKa = 3.21TWYY279 pKa = 8.79GTKK282 pKa = 10.19GVEE285 pKa = 4.43VVFACGGGIFTSAVEE300 pKa = 4.21AAVKK304 pKa = 10.66ADD306 pKa = 3.36GKK308 pKa = 11.18VIGVDD313 pKa = 3.47SDD315 pKa = 3.34QSPIIDD321 pKa = 3.52QSKK324 pKa = 10.45EE325 pKa = 3.79GLTVTSAMKK334 pKa = 10.75GLATTVDD341 pKa = 3.56TVLTEE346 pKa = 4.02IQAGNWNNYY355 pKa = 8.16AGKK358 pKa = 9.89IEE360 pKa = 3.95NLGMVSEE367 pKa = 4.32NPEE370 pKa = 4.03EE371 pKa = 4.65NFVQLPISTTQWGDD385 pKa = 2.74GFTEE389 pKa = 4.05DD390 pKa = 5.33DD391 pKa = 3.65YY392 pKa = 11.37RR393 pKa = 11.84TLVKK397 pKa = 10.65AMYY400 pKa = 9.26NGEE403 pKa = 4.28VEE405 pKa = 4.17VSNDD409 pKa = 2.64IKK411 pKa = 11.13ALPDD415 pKa = 3.42SLSIKK420 pKa = 10.0VNDD423 pKa = 3.77YY424 pKa = 11.56GSIKK428 pKa = 10.63

Molecular weight: 45.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|N2AY61|N2AY61_9FIRM Uncharacterized protein OS=Eubacterium plexicaudatum ASF492 OX=1235802 GN=C823_01138 PE=4 SV=1
MM1 pKa = 7.66KK2 pKa = 8.5MTYY5 pKa = 9.73QPNKK9 pKa = 7.37RR10 pKa = 11.84HH11 pKa = 5.91RR12 pKa = 11.84SKK14 pKa = 10.83VHH16 pKa = 5.68GFRR19 pKa = 11.84KK20 pKa = 9.97RR21 pKa = 11.84MSTAGGRR28 pKa = 11.84KK29 pKa = 8.62VLAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.61GRR39 pKa = 11.84KK40 pKa = 8.87KK41 pKa = 10.59LSAA44 pKa = 3.95

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6154

0

6154

1750650

20

2922

284.5

32.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.352 ± 0.039

1.649 ± 0.015

5.662 ± 0.022

7.485 ± 0.033

4.146 ± 0.026

6.537 ± 0.033

1.916 ± 0.013

7.204 ± 0.029

6.977 ± 0.034

8.874 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.181 ± 0.019

4.437 ± 0.025

3.094 ± 0.019

3.809 ± 0.023

4.885 ± 0.027

5.78 ± 0.025

5.197 ± 0.031

6.403 ± 0.025

0.99 ± 0.011

4.423 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski