Antarctobacter heliothermus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Antarctobacter

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4962 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A222E7N2|A0A222E7N2_9RHOB Deoxyguanosinetriphosphate triphosphohydrolase-like protein OS=Antarctobacter heliothermus OX=74033 GN=dgt PE=3 SV=1
MM1 pKa = 7.78CFLCQSLNPRR11 pKa = 11.84TDD13 pKa = 3.36DD14 pKa = 3.92YY15 pKa = 11.41EE16 pKa = 4.37FHH18 pKa = 6.99GLTGPTAAATVGDD31 pKa = 4.35RR32 pKa = 11.84SSAPASSKK40 pKa = 10.29PVYY43 pKa = 10.78SLDD46 pKa = 3.61QVATQLTHH54 pKa = 7.24GYY56 pKa = 7.12WQSGGGDD63 pKa = 2.69WRR65 pKa = 11.84AFDD68 pKa = 3.8VQAGDD73 pKa = 3.69TLTVNLDD80 pKa = 3.25GLDD83 pKa = 3.77PVGQAAAVAALAAWTAVSGLQFMTTSGDD111 pKa = 3.31ADD113 pKa = 3.43ITFQDD118 pKa = 3.65IDD120 pKa = 3.7GGAYY124 pKa = 9.98SYY126 pKa = 11.35SAVYY130 pKa = 9.12TGWNEE135 pKa = 3.26IAYY138 pKa = 10.25SVVNVHH144 pKa = 7.36ASWQGNADD152 pKa = 3.43YY153 pKa = 10.88YY154 pKa = 10.34YY155 pKa = 9.23QTFIHH160 pKa = 6.87EE161 pKa = 4.39IGHH164 pKa = 6.36ALGLGHH170 pKa = 7.13GGNYY174 pKa = 9.87NGSADD179 pKa = 4.05FDD181 pKa = 4.38SQAHH185 pKa = 5.16YY186 pKa = 11.47ANDD189 pKa = 3.25SWQMSIMSYY198 pKa = 9.67FAQWEE203 pKa = 4.36NPNVDD208 pKa = 3.1ASGMYY213 pKa = 10.58LNSPQMADD221 pKa = 2.72ILAIQNLYY229 pKa = 8.15GTPDD233 pKa = 3.39NVHH236 pKa = 6.24VGNSVYY242 pKa = 11.04GDD244 pKa = 3.74NSNLDD249 pKa = 3.49ATGMDD254 pKa = 4.33LEE256 pKa = 4.66RR257 pKa = 11.84GRR259 pKa = 11.84AVTIFDD265 pKa = 3.72SSGIDD270 pKa = 4.3LIDD273 pKa = 4.12LGSRR277 pKa = 11.84AYY279 pKa = 10.27NQYY282 pKa = 11.17LSLIDD287 pKa = 3.95ATWSNLDD294 pKa = 3.56GYY296 pKa = 10.75VGNFGIARR304 pKa = 11.84GAVIEE309 pKa = 3.99NAITGAGNDD318 pKa = 3.44HH319 pKa = 5.65VTGNTAANDD328 pKa = 3.35IQTGDD333 pKa = 3.52GADD336 pKa = 3.44TVQAGAGDD344 pKa = 3.79DD345 pKa = 4.33TIDD348 pKa = 3.75GGAGEE353 pKa = 4.64DD354 pKa = 3.58TVIFTGASEE363 pKa = 4.84GYY365 pKa = 10.57DD366 pKa = 4.16LIWDD370 pKa = 4.06GALRR374 pKa = 11.84ITDD377 pKa = 3.6TDD379 pKa = 3.87LTDD382 pKa = 4.59GDD384 pKa = 5.15DD385 pKa = 4.11GTDD388 pKa = 3.01VLTDD392 pKa = 3.42VEE394 pKa = 4.78TLSFTDD400 pKa = 4.27GAFGTIQSDD409 pKa = 3.97GAITKK414 pKa = 9.56VRR416 pKa = 11.84LYY418 pKa = 9.67KK419 pKa = 10.33TGSSLTEE426 pKa = 3.58EE427 pKa = 4.17TLEE430 pKa = 3.91VDD432 pKa = 4.22VANSHH437 pKa = 6.45DD438 pKa = 3.63WQSIARR444 pKa = 11.84SFTDD448 pKa = 3.14TGQWASQTNTYY459 pKa = 11.38DD460 pKa = 3.01NGRR463 pKa = 11.84VLQISFADD471 pKa = 3.93GLRR474 pKa = 11.84AQSTMTDD481 pKa = 3.26TANVYY486 pKa = 9.94SWASYY491 pKa = 9.02TDD493 pKa = 3.41IYY495 pKa = 11.22DD496 pKa = 3.41EE497 pKa = 4.25TGARR501 pKa = 11.84IINSYY506 pKa = 8.3TWDD509 pKa = 3.44SGKK512 pKa = 9.51MVDD515 pKa = 4.33VYY517 pKa = 11.33YY518 pKa = 10.62DD519 pKa = 3.48SEE521 pKa = 4.27GARR524 pKa = 11.84IGSLVIDD531 pKa = 4.82GGDD534 pKa = 3.17QFVWHH539 pKa = 6.92SIARR543 pKa = 11.84SYY545 pKa = 11.73DD546 pKa = 3.3STGTLTEE553 pKa = 4.05QNNLFDD559 pKa = 5.76DD560 pKa = 4.76GSEE563 pKa = 4.02QVISYY568 pKa = 9.73TDD570 pKa = 3.68GVRR573 pKa = 11.84SGISMTDD580 pKa = 3.41GADD583 pKa = 3.21KK584 pKa = 10.9AIWSSYY590 pKa = 8.26TDD592 pKa = 3.25TYY594 pKa = 11.25DD595 pKa = 3.09TATGAKK601 pKa = 9.28VSRR604 pKa = 11.84LMIYY608 pKa = 10.6DD609 pKa = 4.1DD610 pKa = 3.64GRR612 pKa = 11.84EE613 pKa = 3.98IEE615 pKa = 4.07TGYY618 pKa = 11.33DD619 pKa = 3.05NGQMVSQTITDD630 pKa = 3.87GADD633 pKa = 3.01DD634 pKa = 4.58FVWHH638 pKa = 5.88STTRR642 pKa = 11.84NYY644 pKa = 11.04DD645 pKa = 3.37SEE647 pKa = 4.86GMLDD651 pKa = 3.94SQVDD655 pKa = 3.89TYY657 pKa = 11.94DD658 pKa = 3.59DD659 pKa = 3.78GRR661 pKa = 11.84VMEE664 pKa = 4.89IEE666 pKa = 4.25FEE668 pKa = 4.09NGLRR672 pKa = 11.84SSSIQTDD679 pKa = 3.45VADD682 pKa = 4.7AYY684 pKa = 10.23AWASATEE691 pKa = 4.43TYY693 pKa = 10.74DD694 pKa = 4.49DD695 pKa = 4.12SGTLIEE701 pKa = 6.08RR702 pKa = 11.84VTHH705 pKa = 6.46WDD707 pKa = 3.75DD708 pKa = 3.73GSEE711 pKa = 4.2SAVTYY716 pKa = 10.55GDD718 pKa = 4.16SIAA721 pKa = 4.57

Molecular weight: 77.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A222E225|A0A222E225_9RHOB D-alanyl-D-alanine carboxypeptidase DacF OS=Antarctobacter heliothermus OX=74033 GN=dacF PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.5ILNARR34 pKa = 11.84RR35 pKa = 11.84LRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4962

0

4962

1553299

29

3565

313.0

33.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.146 ± 0.048

0.944 ± 0.011

6.335 ± 0.044

5.591 ± 0.036

3.675 ± 0.02

8.804 ± 0.051

2.08 ± 0.02

5.034 ± 0.029

2.981 ± 0.032

10.128 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.791 ± 0.019

2.55 ± 0.022

5.202 ± 0.031

3.195 ± 0.02

6.722 ± 0.041

5.109 ± 0.023

5.684 ± 0.035

7.418 ± 0.032

1.409 ± 0.016

2.204 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski