Cuspidothrix issatschenkoi CHARLIE-1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Nostocales; Aphanizomenonaceae; Cuspidothrix; Cuspidothrix issatschenkoi

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3876 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S6CUH9|A0A2S6CUH9_9CYAN Phytoene/squalene synthase family protein OS=Cuspidothrix issatschenkoi CHARLIE-1 OX=2052836 GN=CUN59_10435 PE=4 SV=1
MM1 pKa = 7.7PLQVEE6 pKa = 5.47LYY8 pKa = 11.02LEE10 pKa = 4.66DD11 pKa = 4.75CVDD14 pKa = 4.12DD15 pKa = 4.44SSAIAINISDD25 pKa = 4.29NYY27 pKa = 9.39PIPGEE32 pKa = 3.91TWEE35 pKa = 4.26NWFQQWLEE43 pKa = 3.84ILQEE47 pKa = 4.2YY48 pKa = 10.43LPTAPSYY55 pKa = 10.41EE56 pKa = 3.6ISLRR60 pKa = 11.84LTNDD64 pKa = 3.0TEE66 pKa = 4.29MQTLNSQYY74 pKa = 10.37RR75 pKa = 11.84QQDD78 pKa = 3.06KK79 pKa = 8.92PTDD82 pKa = 3.62VLAFASLEE90 pKa = 3.89ADD92 pKa = 4.06LPQSEE97 pKa = 4.99KK98 pKa = 10.27MLASMPLYY106 pKa = 10.6LGDD109 pKa = 3.68VVVSIDD115 pKa = 3.33TAQRR119 pKa = 11.84QAQQQEE125 pKa = 4.5HH126 pKa = 6.08SLSTEE131 pKa = 4.17LAWLTAHH138 pKa = 7.33GLLHH142 pKa = 6.68LLGWDD147 pKa = 3.75HH148 pKa = 7.39PDD150 pKa = 3.41EE151 pKa = 4.49EE152 pKa = 5.46SLMEE156 pKa = 4.22MLTQQVALLKK166 pKa = 10.52EE167 pKa = 3.7IGINIDD173 pKa = 3.33LDD175 pKa = 3.88LL176 pKa = 5.93

Molecular weight: 20.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S6CW96|A0A2S6CW96_9CYAN UDP-N-acetylglucosamine 2-epimerase (Non-hydrolyzing) OS=Cuspidothrix issatschenkoi CHARLIE-1 OX=2052836 GN=CUN59_06810 PE=3 SV=1
MM1 pKa = 7.31KK2 pKa = 10.03PNYY5 pKa = 9.07RR6 pKa = 11.84RR7 pKa = 11.84CISCRR12 pKa = 11.84QVNLKK17 pKa = 10.15QEE19 pKa = 4.25FWRR22 pKa = 11.84IVRR25 pKa = 11.84VFPSGNVQLDD35 pKa = 3.21QGMGRR40 pKa = 11.84SAYY43 pKa = 8.97ICRR46 pKa = 11.84QASCLQLAQKK56 pKa = 10.36KK57 pKa = 9.43NRR59 pKa = 11.84LGRR62 pKa = 11.84CLWPARR68 pKa = 11.84RR69 pKa = 11.84SLQGKK74 pKa = 9.07VPDD77 pKa = 4.16TLYY80 pKa = 9.08QTLWQRR86 pKa = 11.84LNHH89 pKa = 6.38SDD91 pKa = 3.24SS92 pKa = 3.46

Molecular weight: 10.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3876

0

3876

1187661

27

9593

306.4

34.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.32 ± 0.046

0.996 ± 0.013

4.9 ± 0.027

6.418 ± 0.038

3.974 ± 0.024

6.42 ± 0.04

1.895 ± 0.02

7.608 ± 0.036

5.514 ± 0.038

10.854 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.914 ± 0.018

4.886 ± 0.033

4.57 ± 0.03

5.265 ± 0.037

4.67 ± 0.026

6.187 ± 0.037

5.695 ± 0.033

6.305 ± 0.032

1.39 ± 0.016

3.218 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski