Parvularcula sp. SM1705

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Parvularculales; Parvularculaceae; Parvularcula; unclassified Parvularcula

Average proteome isoelectric point is 5.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3100 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A371RGJ9|A0A371RGJ9_9PROT Uncharacterized protein OS=Parvularcula sp. SM1705 OX=2292771 GN=DX908_04355 PE=4 SV=1
MM1 pKa = 7.44IYY3 pKa = 10.2YY4 pKa = 9.49YY5 pKa = 11.16VSALEE10 pKa = 4.54GDD12 pKa = 4.12DD13 pKa = 4.48LNSGEE18 pKa = 4.56EE19 pKa = 4.2GAPLASLLALWAKK32 pKa = 10.66LEE34 pKa = 4.03SLRR37 pKa = 11.84NSEE40 pKa = 3.8VTAFIAAGDD49 pKa = 3.97YY50 pKa = 10.47TDD52 pKa = 3.5EE53 pKa = 4.56HH54 pKa = 7.75LDD56 pKa = 3.45LGFFGEE62 pKa = 4.16NALLEE67 pKa = 4.51FEE69 pKa = 4.64AGTRR73 pKa = 11.84ILWTEE78 pKa = 4.0GVSRR82 pKa = 11.84SGIQSGVGATVTVHH96 pKa = 6.68GNGLVIDD103 pKa = 4.65GFATGTGNGLGTYY116 pKa = 8.88KK117 pKa = 10.19GTMIAYY123 pKa = 8.6DD124 pKa = 3.43VSISNVRR131 pKa = 11.84DD132 pKa = 4.05GISAHH137 pKa = 5.76EE138 pKa = 4.33VGAHH142 pKa = 5.6LEE144 pKa = 4.23AYY146 pKa = 9.75RR147 pKa = 11.84VTVTDD152 pKa = 3.07AHH154 pKa = 6.71KK155 pKa = 10.57YY156 pKa = 9.93AVVHH160 pKa = 5.49VNGATSYY167 pKa = 11.29QEE169 pKa = 4.82DD170 pKa = 5.06CFLQPADD177 pKa = 3.71DD178 pKa = 4.18AVGIGYY184 pKa = 9.65VVDD187 pKa = 4.4GEE189 pKa = 4.64HH190 pKa = 5.69TFVRR194 pKa = 11.84CEE196 pKa = 3.98VVASPNGLVNRR207 pKa = 11.84LNTFEE212 pKa = 4.54GVTFIDD218 pKa = 3.71STLGSEE224 pKa = 4.58DD225 pKa = 3.38VPIIITGDD233 pKa = 3.37AVIMGGFANVFRR245 pKa = 11.84EE246 pKa = 4.47GVGLVQIDD254 pKa = 4.0ALNTSSVVFKK264 pKa = 10.82GAEE267 pKa = 3.72LDD269 pKa = 4.2DD270 pKa = 3.97YY271 pKa = 11.93LEE273 pKa = 4.77GGFANDD279 pKa = 4.01EE280 pKa = 4.2LTGGDD285 pKa = 3.81GADD288 pKa = 3.46TLVGGNGNDD297 pKa = 4.03ILIGDD302 pKa = 3.85QGQDD306 pKa = 3.37YY307 pKa = 11.39LMGGDD312 pKa = 4.77GNDD315 pKa = 3.7LLIGDD320 pKa = 5.31GIFF323 pKa = 3.68

Molecular weight: 33.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A371REX1|A0A371REX1_9PROT Chorismate mutase OS=Parvularcula sp. SM1705 OX=2292771 GN=DX908_01105 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLKK11 pKa = 9.91RR12 pKa = 11.84KK13 pKa = 8.91RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.5GFRR19 pKa = 11.84ARR21 pKa = 11.84KK22 pKa = 7.81ATVGGRR28 pKa = 11.84KK29 pKa = 8.81ILANRR34 pKa = 11.84RR35 pKa = 11.84SKK37 pKa = 10.87GRR39 pKa = 11.84AKK41 pKa = 10.73LSAA44 pKa = 3.92

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3100

0

3100

1024218

29

5372

330.4

35.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.306 ± 0.063

0.771 ± 0.013

6.432 ± 0.057

6.802 ± 0.043

3.969 ± 0.033

8.75 ± 0.058

1.917 ± 0.026

5.476 ± 0.031

3.485 ± 0.04

9.64 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.523 ± 0.023

2.829 ± 0.036

4.979 ± 0.037

2.977 ± 0.024

6.336 ± 0.049

5.7 ± 0.038

5.588 ± 0.041

6.753 ± 0.033

1.27 ± 0.021

2.497 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski