Amino acid dipepetide frequency for Methanococcus maripaludis (strain S2 / LL)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	3.922AlaAla: 3.922 ± 0.136	0.856AlaCys: 0.856 ± 0.051	3.043AlaAsp: 3.043 ± 0.092	4.295AlaGlu: 4.295 ± 0.11	2.572AlaPhe: 2.572 ± 0.081	4.187AlaGly: 4.187 ± 0.116	0.891AlaHis: 0.891 ± 0.045	5.583AlaIle: 5.583 ± 0.126	4.912AlaLys: 4.912 ± 0.12	5.612AlaLeu: 5.612 ± 0.118	1.667AlaMet: 1.667 ± 0.068	2.169AlaAsn: 2.169 ± 0.071	1.814AlaPro: 1.814 ± 0.069	1.178AlaGln: 1.178 ± 0.058	1.869AlaArg: 1.869 ± 0.063	3.31AlaSer: 3.31 ± 0.095	2.756AlaThr: 2.756 ± 0.078	4.994AlaVal: 4.994 ± 0.123	0.414AlaTrp: 0.414 ± 0.033	2.049AlaTyr: 2.049 ± 0.056	0.0AlaXaa: 0.0 ± 0.0
Cys	0.84CysAla: 0.84 ± 0.045	0.206CysCys: 0.206 ± 0.024	0.83CysAsp: 0.83 ± 0.046	0.915CysGlu: 0.915 ± 0.048	0.418CysPhe: 0.418 ± 0.035	1.612CysGly: 1.612 ± 0.074	0.228CysHis: 0.228 ± 0.023	1.148CysIle: 1.148 ± 0.049	0.989CysLys: 0.989 ± 0.052	0.879CysLeu: 0.879 ± 0.037	0.355CysMet: 0.355 ± 0.027	0.616CysAsn: 0.616 ± 0.04	1.035CysPro: 1.035 ± 0.071	0.21CysGln: 0.21 ± 0.021	0.369CysArg: 0.369 ± 0.026	0.842CysSer: 0.842 ± 0.043	0.697CysThr: 0.697 ± 0.044	0.919CysVal: 0.919 ± 0.058	0.077CysTrp: 0.077 ± 0.013	0.483CysTyr: 0.483 ± 0.032	0.0CysXaa: 0.0 ± 0.0
Asp	3.408AspAla: 3.408 ± 0.099	0.728AspCys: 0.728 ± 0.044	2.852AspAsp: 2.852 ± 0.085	5.21AspGlu: 5.21 ± 0.108	2.96AspPhe: 2.96 ± 0.083	3.388AspGly: 3.388 ± 0.1	0.746AspHis: 0.746 ± 0.039	5.881AspIle: 5.881 ± 0.111	3.854AspLys: 3.854 ± 0.107	5.53AspLeu: 5.53 ± 0.118	1.394AspMet: 1.394 ± 0.058	2.33AspAsn: 2.33 ± 0.076	1.865AspPro: 1.865 ± 0.068	0.775AspGln: 0.775 ± 0.035	1.702AspArg: 1.702 ± 0.064	3.282AspSer: 3.282 ± 0.091	2.638AspThr: 2.638 ± 0.088	4.503AspVal: 4.503 ± 0.092	0.41AspTrp: 0.41 ± 0.034	2.564AspTyr: 2.564 ± 0.078	0.0AspXaa: 0.0 ± 0.0
Glu	3.532GluAla: 3.532 ± 0.106	0.923GluCys: 0.923 ± 0.052	4.179GluAsp: 4.179 ± 0.088	6.741GluGlu: 6.741 ± 0.137	3.606GluPhe: 3.606 ± 0.088	3.954GluGly: 3.954 ± 0.092	1.137GluHis: 1.137 ± 0.049	8.85GluIle: 8.85 ± 0.168	9.25GluLys: 9.25 ± 0.19	7.98GluLeu: 7.98 ± 0.139	2.336GluMet: 2.336 ± 0.079	6.647GluAsn: 6.647 ± 0.135	1.802GluPro: 1.802 ± 0.074	1.521GluGln: 1.521 ± 0.061	2.468GluArg: 2.468 ± 0.083	4.085GluSer: 4.085 ± 0.092	3.653GluThr: 3.653 ± 0.097	4.971GluVal: 4.971 ± 0.104	0.43GluTrp: 0.43 ± 0.032	3.243GluTyr: 3.243 ± 0.084	0.0GluXaa: 0.0 ± 0.0
Phe	2.226PheAla: 2.226 ± 0.073	0.607PheCys: 0.607 ± 0.033	2.613PheAsp: 2.613 ± 0.072	4.132PheGlu: 4.132 ± 0.096	1.637PhePhe: 1.637 ± 0.062	3.594PheGly: 3.594 ± 0.112	0.546PheHis: 0.546 ± 0.035	3.139PheIle: 3.139 ± 0.09	3.61PheLys: 3.61 ± 0.103	4.146PheLeu: 4.146 ± 0.129	1.029PheMet: 1.029 ± 0.048	2.124PheAsn: 2.124 ± 0.069	1.249PhePro: 1.249 ± 0.054	0.768PheGln: 0.768 ± 0.042	1.109PheArg: 1.109 ± 0.05	3.076PheSer: 3.076 ± 0.092	1.845PheThr: 1.845 ± 0.061	2.841PheVal: 2.841 ± 0.075	0.322PheTrp: 0.322 ± 0.027	1.388PheTyr: 1.388 ± 0.051	0.0PheXaa: 0.0 ± 0.0
Gly	4.264GlyAla: 4.264 ± 0.109	1.205GlyCys: 1.205 ± 0.064	3.437GlyAsp: 3.437 ± 0.094	4.122GlyGlu: 4.122 ± 0.108	3.041GlyPhe: 3.041 ± 0.088	4.552GlyGly: 4.552 ± 0.124	1.172GlyHis: 1.172 ± 0.047	7.287GlyIle: 7.287 ± 0.125	5.85GlyLys: 5.85 ± 0.129	5.862GlyLeu: 5.862 ± 0.104	1.845GlyMet: 1.845 ± 0.063	3.553GlyAsn: 3.553 ± 0.101	1.596GlyPro: 1.596 ± 0.057	1.327GlyGln: 1.327 ± 0.047	2.338GlyArg: 2.338 ± 0.069	3.836GlySer: 3.836 ± 0.1	4.007GlyThr: 4.007 ± 0.105	4.896GlyVal: 4.896 ± 0.113	0.446GlyTrp: 0.446 ± 0.037	2.899GlyTyr: 2.899 ± 0.088	0.0GlyXaa: 0.0 ± 0.0
His	0.972HisAla: 0.972 ± 0.049	0.32HisCys: 0.32 ± 0.025	0.787HisAsp: 0.787 ± 0.033	1.091HisGlu: 1.091 ± 0.056	0.63HisPhe: 0.63 ± 0.035	1.107HisGly: 1.107 ± 0.045	0.32HisHis: 0.32 ± 0.034	1.292HisIle: 1.292 ± 0.047	1.042HisLys: 1.042 ± 0.047	1.274HisLeu: 1.274 ± 0.053	0.395HisMet: 0.395 ± 0.026	0.715HisAsn: 0.715 ± 0.045	0.887HisPro: 0.887 ± 0.047	0.312HisGln: 0.312 ± 0.028	0.501HisArg: 0.501 ± 0.034	0.852HisSer: 0.852 ± 0.047	0.724HisThr: 0.724 ± 0.041	1.088HisVal: 1.088 ± 0.041	0.084HisTrp: 0.084 ± 0.013	0.62HisTyr: 0.62 ± 0.036	0.0HisXaa: 0.0 ± 0.0
Ile	5.644IleAla: 5.644 ± 0.109	1.207IleCys: 1.207 ± 0.048	5.693IleAsp: 5.693 ± 0.116	7.432IleGlu: 7.432 ± 0.141	3.891IlePhe: 3.891 ± 0.108	6.276IleGly: 6.276 ± 0.127	1.498IleHis: 1.498 ± 0.06	8.011IleIle: 8.011 ± 0.141	7.179IleLys: 7.179 ± 0.115	9.097IleLeu: 9.097 ± 0.181	2.126IleMet: 2.126 ± 0.075	4.906IleAsn: 4.906 ± 0.115	4.044IlePro: 4.044 ± 0.103	1.812IleGln: 1.812 ± 0.063	2.658IleArg: 2.658 ± 0.083	7.432IleSer: 7.432 ± 0.156	4.927IleThr: 4.927 ± 0.115	6.245IleVal: 6.245 ± 0.114	0.552IleTrp: 0.552 ± 0.038	3.375IleTyr: 3.375 ± 0.095	0.0IleXaa: 0.0 ± 0.0
Lys	4.278LysAla: 4.278 ± 0.109	1.144LysCys: 1.144 ± 0.062	5.045LysAsp: 5.045 ± 0.095	7.943LysGlu: 7.943 ± 0.17	3.027LysPhe: 3.027 ± 0.078	4.45LysGly: 4.45 ± 0.117	1.335LysHis: 1.335 ± 0.053	9.698LysIle: 9.698 ± 0.155	8.726LysLys: 8.726 ± 0.152	7.601LysLeu: 7.601 ± 0.134	2.872LysMet: 2.872 ± 0.071	6.519LysAsn: 6.519 ± 0.148	2.362LysPro: 2.362 ± 0.071	1.565LysGln: 1.565 ± 0.052	2.954LysArg: 2.954 ± 0.085	4.768LysSer: 4.768 ± 0.11	4.87LysThr: 4.87 ± 0.1	5.055LysVal: 5.055 ± 0.112	0.493LysTrp: 0.493 ± 0.039	3.783LysTyr: 3.783 ± 0.099	0.0LysXaa: 0.0 ± 0.0
Leu	5.257LeuAla: 5.257 ± 0.117	1.086LeuCys: 1.086 ± 0.056	5.292LeuAsp: 5.292 ± 0.117	7.548LeuGlu: 7.548 ± 0.129	3.526LeuPhe: 3.526 ± 0.12	6.343LeuGly: 6.343 ± 0.133	1.127LeuHis: 1.127 ± 0.051	7.939LeuIle: 7.939 ± 0.17	10.263LeuLys: 10.263 ± 0.169	7.413LeuLeu: 7.413 ± 0.176	2.487LeuMet: 2.487 ± 0.075	6.125LeuAsn: 6.125 ± 0.126	2.784LeuPro: 2.784 ± 0.081	1.586LeuGln: 1.586 ± 0.063	2.911LeuArg: 2.911 ± 0.088	6.362LeuSer: 6.362 ± 0.134	4.23LeuThr: 4.23 ± 0.098	5.424LeuVal: 5.424 ± 0.117	0.516LeuTrp: 0.516 ± 0.035	2.662LeuTyr: 2.662 ± 0.081	0.0LeuXaa: 0.0 ± 0.0
Met	1.963MetAla: 1.963 ± 0.068	0.312MetCys: 0.312 ± 0.023	2.073MetAsp: 2.073 ± 0.066	1.998MetGlu: 1.998 ± 0.062	0.915MetPhe: 0.915 ± 0.042	2.102MetGly: 2.102 ± 0.066	0.463MetHis: 0.463 ± 0.033	2.102MetIle: 2.102 ± 0.063	2.218MetLys: 2.218 ± 0.071	2.014MetLeu: 2.014 ± 0.063	0.681MetMet: 0.681 ± 0.039	1.443MetAsn: 1.443 ± 0.054	0.97MetPro: 0.97 ± 0.049	0.518MetGln: 0.518 ± 0.032	0.766MetArg: 0.766 ± 0.042	1.492MetSer: 1.492 ± 0.063	1.207MetThr: 1.207 ± 0.055	2.038MetVal: 2.038 ± 0.069	0.149MetTrp: 0.149 ± 0.018	0.811MetTyr: 0.811 ± 0.04	0.0MetXaa: 0.0 ± 0.0
Asn	3.151AsnAla: 3.151 ± 0.092	0.799AsnCys: 0.799 ± 0.043	2.801AsnAsp: 2.801 ± 0.082	4.399AsnGlu: 4.399 ± 0.103	2.34AsnPhe: 2.34 ± 0.074	3.512AsnGly: 3.512 ± 0.103	0.799AsnHis: 0.799 ± 0.047	5.54AsnIle: 5.54 ± 0.12	4.011AsnLys: 4.011 ± 0.099	5.573AsnLeu: 5.573 ± 0.133	1.529AsnMet: 1.529 ± 0.053	2.976AsnAsn: 2.976 ± 0.112	2.607AsnPro: 2.607 ± 0.071	1.095AsnGln: 1.095 ± 0.039	1.594AsnArg: 1.594 ± 0.06	3.757AsnSer: 3.757 ± 0.109	2.788AsnThr: 2.788 ± 0.085	4.016AsnVal: 4.016 ± 0.089	0.43AsnTrp: 0.43 ± 0.029	2.652AsnTyr: 2.652 ± 0.092	0.0AsnXaa: 0.0 ± 0.0
Pro	1.845ProAla: 1.845 ± 0.063	0.52ProCys: 0.52 ± 0.035	1.879ProAsp: 1.879 ± 0.065	3.716ProGlu: 3.716 ± 0.085	1.514ProPhe: 1.514 ± 0.053	2.014ProGly: 2.014 ± 0.077	0.636ProHis: 0.636 ± 0.03	2.976ProIle: 2.976 ± 0.08	3.064ProLys: 3.064 ± 0.085	2.917ProLeu: 2.917 ± 0.088	0.783ProMet: 0.783 ± 0.036	1.502ProAsn: 1.502 ± 0.056	0.874ProPro: 0.874 ± 0.046	0.709ProGln: 0.709 ± 0.042	1.017ProArg: 1.017 ± 0.045	1.841ProSer: 1.841 ± 0.061	1.729ProThr: 1.729 ± 0.069	2.962ProVal: 2.962 ± 0.084	0.214ProTrp: 0.214 ± 0.022	1.274ProTyr: 1.274 ± 0.045	0.0ProXaa: 0.0 ± 0.0
Gln	1.035GlnAla: 1.035 ± 0.054	0.255GlnCys: 0.255 ± 0.026	0.913GlnAsp: 0.913 ± 0.045	1.272GlnGlu: 1.272 ± 0.053	0.758GlnPhe: 0.758 ± 0.043	1.105GlnGly: 1.105 ± 0.046	0.255GlnHis: 0.255 ± 0.024	1.822GlnIle: 1.822 ± 0.052	2.067GlnLys: 2.067 ± 0.07	1.598GlnLeu: 1.598 ± 0.064	0.595GlnMet: 0.595 ± 0.035	1.431GlnAsn: 1.431 ± 0.057	0.438GlnPro: 0.438 ± 0.029	0.41GlnGln: 0.41 ± 0.03	0.713GlnArg: 0.713 ± 0.041	1.072GlnSer: 1.072 ± 0.049	0.844GlnThr: 0.844 ± 0.047	1.27GlnVal: 1.27 ± 0.048	0.13GlnTrp: 0.13 ± 0.015	0.722GlnTyr: 0.722 ± 0.036	0.0GlnXaa: 0.0 ± 0.0
Arg	1.998ArgAla: 1.998 ± 0.062	0.43ArgCys: 0.43 ± 0.035	1.676ArgAsp: 1.676 ± 0.069	2.458ArgGlu: 2.458 ± 0.089	1.28ArgPhe: 1.28 ± 0.045	2.018ArgGly: 2.018 ± 0.074	0.534ArgHis: 0.534 ± 0.037	2.958ArgIle: 2.958 ± 0.083	2.772ArgLys: 2.772 ± 0.089	2.574ArgLeu: 2.574 ± 0.071	0.934ArgMet: 0.934 ± 0.047	1.722ArgAsn: 1.722 ± 0.053	1.044ArgPro: 1.044 ± 0.055	0.585ArgGln: 0.585 ± 0.039	1.201ArgArg: 1.201 ± 0.059	1.553ArgSer: 1.553 ± 0.062	1.527ArgThr: 1.527 ± 0.06	2.189ArgVal: 2.189 ± 0.066	0.234ArgTrp: 0.234 ± 0.022	1.272ArgTyr: 1.272 ± 0.051	0.0ArgXaa: 0.0 ± 0.0
Ser	3.398SerAla: 3.398 ± 0.088	0.754SerCys: 0.754 ± 0.046	3.514SerAsp: 3.514 ± 0.084	5.848SerGlu: 5.848 ± 0.118	2.672SerPhe: 2.672 ± 0.087	4.99SerGly: 4.99 ± 0.118	0.864SerHis: 0.864 ± 0.039	5.669SerIle: 5.669 ± 0.135	6.433SerLys: 6.433 ± 0.151	5.181SerLeu: 5.181 ± 0.12	1.545SerMet: 1.545 ± 0.065	3.198SerAsn: 3.198 ± 0.099	1.936SerPro: 1.936 ± 0.062	1.166SerGln: 1.166 ± 0.051	1.991SerArg: 1.991 ± 0.064	3.771SerSer: 3.771 ± 0.121	2.907SerThr: 2.907 ± 0.088	4.038SerVal: 4.038 ± 0.095	0.495SerTrp: 0.495 ± 0.031	2.197SerTyr: 2.197 ± 0.08	0.0SerXaa: 0.0 ± 0.0
Thr	3.435ThrAla: 3.435 ± 0.103	0.646ThrCys: 0.646 ± 0.041	2.593ThrAsp: 2.593 ± 0.073	3.496ThrGlu: 3.496 ± 0.087	1.983ThrPhe: 1.983 ± 0.069	4.179ThrGly: 4.179 ± 0.111	0.83ThrHis: 0.83 ± 0.043	4.456ThrIle: 4.456 ± 0.096	3.608ThrLys: 3.608 ± 0.097	4.539ThrLeu: 4.539 ± 0.099	1.019ThrMet: 1.019 ± 0.05	2.297ThrAsn: 2.297 ± 0.072	2.291ThrPro: 2.291 ± 0.074	0.991ThrGln: 0.991 ± 0.044	1.396ThrArg: 1.396 ± 0.05	3.037ThrSer: 3.037 ± 0.073	2.691ThrThr: 2.691 ± 0.09	3.963ThrVal: 3.963 ± 0.1	0.338ThrTrp: 0.338 ± 0.033	1.865ThrTyr: 1.865 ± 0.068	0.0ThrXaa: 0.0 ± 0.0
Val	4.215ValAla: 4.215 ± 0.106	1.054ValCys: 1.054 ± 0.053	3.995ValAsp: 3.995 ± 0.092	5.418ValGlu: 5.418 ± 0.11	3.074ValPhe: 3.074 ± 0.094	4.692ValGly: 4.692 ± 0.099	1.017ValHis: 1.017 ± 0.053	6.172ValIle: 6.172 ± 0.113	5.642ValLys: 5.642 ± 0.113	6.861ValLeu: 6.861 ± 0.125	1.61ValMet: 1.61 ± 0.051	3.5ValAsn: 3.5 ± 0.078	2.615ValPro: 2.615 ± 0.072	1.284ValGln: 1.284 ± 0.051	1.977ValArg: 1.977 ± 0.064	5.102ValSer: 5.102 ± 0.108	3.471ValThr: 3.471 ± 0.098	5.328ValVal: 5.328 ± 0.127	0.369ValTrp: 0.369 ± 0.026	2.419ValTyr: 2.419 ± 0.078	0.0ValXaa: 0.0 ± 0.0
Trp	0.465TrpAla: 0.465 ± 0.031	0.106TrpCys: 0.106 ± 0.019	0.461TrpAsp: 0.461 ± 0.034	0.434TrpGlu: 0.434 ± 0.036	0.253TrpPhe: 0.253 ± 0.022	0.448TrpGly: 0.448 ± 0.03	0.096TrpHis: 0.096 ± 0.013	0.522TrpIle: 0.522 ± 0.04	0.542TrpLys: 0.542 ± 0.035	0.508TrpLeu: 0.508 ± 0.042	0.188TrpMet: 0.188 ± 0.021	0.459TrpAsn: 0.459 ± 0.033	0.173TrpPro: 0.173 ± 0.019	0.153TrpGln: 0.153 ± 0.018	0.241TrpArg: 0.241 ± 0.021	0.294TrpSer: 0.294 ± 0.029	0.33TrpThr: 0.33 ± 0.027	0.481TrpVal: 0.481 ± 0.03	0.073TrpTrp: 0.073 ± 0.013	0.275TrpTyr: 0.275 ± 0.027	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.244TyrAla: 2.244 ± 0.068	0.51TyrCys: 0.51 ± 0.034	2.344TyrAsp: 2.344 ± 0.071	2.843TyrGlu: 2.843 ± 0.074	1.904TyrPhe: 1.904 ± 0.066	3.013TyrGly: 3.013 ± 0.086	0.518TyrHis: 0.518 ± 0.036	2.962TyrIle: 2.962 ± 0.088	2.456TyrLys: 2.456 ± 0.074	3.722TyrLeu: 3.722 ± 0.099	0.842TyrMet: 0.842 ± 0.038	2.136TyrAsn: 2.136 ± 0.079	1.596TyrPro: 1.596 ± 0.055	0.742TyrGln: 0.742 ± 0.039	1.184TyrArg: 1.184 ± 0.058	2.807TyrSer: 2.807 ± 0.073	1.814TyrThr: 1.814 ± 0.062	2.595TyrVal: 2.595 ± 0.084	0.322TyrTrp: 0.322 ± 0.021	1.651TyrTyr: 1.651 ± 0.064	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 1722 proteins (490599 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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