Bacillus phage SP01 (Bacteriophage SP01)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Spounavirinae; Okubovirus

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 204 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B6V2S7|B6V2S7_BPSP1 Gp21.8 OS=Bacillus phage SP01 OX=10685 GN=21.8 PE=4 SV=1
MM1 pKa = 7.83SDD3 pKa = 3.62NSNKK7 pKa = 10.01TITISVEE14 pKa = 3.94EE15 pKa = 4.26YY16 pKa = 10.62DD17 pKa = 4.67NSIKK21 pKa = 10.16TITILLEE28 pKa = 4.44EE29 pKa = 4.37YY30 pKa = 9.97QHH32 pKa = 7.11LIDD35 pKa = 4.63RR36 pKa = 11.84VSFLDD41 pKa = 3.62CLEE44 pKa = 4.21STGVDD49 pKa = 2.7NWSGYY54 pKa = 10.91GEE56 pKa = 4.11AQEE59 pKa = 4.82MYY61 pKa = 10.69NEE63 pKa = 4.43DD64 pKa = 3.73SEE66 pKa = 5.12EE67 pKa = 4.14EE68 pKa = 4.15TGGYY72 pKa = 9.38

Molecular weight: 8.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B6V306|B6V306_BPSP1 Gp34.56 OS=Bacillus phage SP01 OX=10685 GN=34.56 PE=4 SV=1
MM1 pKa = 7.66GNFCTRR7 pKa = 11.84YY8 pKa = 7.53YY9 pKa = 10.84SEE11 pKa = 4.48YY12 pKa = 10.98GEE14 pKa = 4.83HH15 pKa = 6.81LWTSTKK21 pKa = 10.3LPKK24 pKa = 10.08YY25 pKa = 10.31RR26 pKa = 11.84PFKK29 pKa = 10.91SKK31 pKa = 10.86SKK33 pKa = 10.49DD34 pKa = 2.91KK35 pKa = 9.18HH36 pKa = 4.85TEE38 pKa = 4.11YY39 pKa = 10.54IHH41 pKa = 6.26LTTGGGIMATDD52 pKa = 3.71YY53 pKa = 10.65FVKK56 pKa = 10.5FCRR59 pKa = 11.84ASGKK63 pKa = 9.56IVHH66 pKa = 6.76IKK68 pKa = 10.41NDD70 pKa = 3.22GRR72 pKa = 11.84YY73 pKa = 8.54SPLRR77 pKa = 11.84KK78 pKa = 8.99RR79 pKa = 11.84IYY81 pKa = 9.6RR82 pKa = 11.84RR83 pKa = 11.84RR84 pKa = 11.84RR85 pKa = 3.14

Molecular weight: 10.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

204

0

204

39568

17

1129

194.0

22.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.408 ± 0.176

0.973 ± 0.076

6.664 ± 0.148

8.193 ± 0.293

3.541 ± 0.109

6.74 ± 0.206

1.888 ± 0.099

6.412 ± 0.168

8.067 ± 0.3

8.103 ± 0.178

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.767 ± 0.094

4.883 ± 0.186

3.351 ± 0.119

3.318 ± 0.116

4.491 ± 0.118

6.386 ± 0.189

6.071 ± 0.236

7.256 ± 0.197

1.132 ± 0.066

4.355 ± 0.136
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski