Solitalea canadensis (strain ATCC 29591 / DSM 3403 / NBRC 15130 / NCIMB 12057 / USAM 9D) (Flexibacter canadensis)

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Sphingobacteriaceae; Solitalea; Solitalea canadensis

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4304 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H8KQG8|H8KQG8_SOLCM Uncharacterized protein OS=Solitalea canadensis (strain ATCC 29591 / DSM 3403 / NBRC 15130 / NCIMB 12057 / USAM 9D) OX=929556 GN=Solca_1509 PE=4 SV=1
MM1 pKa = 7.36QDD3 pKa = 3.17IITALVEE10 pKa = 4.09MEE12 pKa = 4.29EE13 pKa = 4.49LCPEE17 pKa = 4.73LYY19 pKa = 10.81LFIDD23 pKa = 4.05EE24 pKa = 4.55TPQLISDD31 pKa = 3.95SDD33 pKa = 3.47ITEE36 pKa = 4.41NGPSIIALQEE46 pKa = 3.67YY47 pKa = 10.09LQTLKK52 pKa = 10.96CQLVKK57 pKa = 10.96AGNTSKK63 pKa = 11.16LIYY66 pKa = 10.11NAA68 pKa = 4.12

Molecular weight: 7.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H8KNA7|H8KNA7_SOLCM Uncharacterized protein OS=Solitalea canadensis (strain ATCC 29591 / DSM 3403 / NBRC 15130 / NCIMB 12057 / USAM 9D) OX=929556 GN=Solca_4450 PE=4 SV=1
MM1 pKa = 7.3EE2 pKa = 6.13RR3 pKa = 11.84IGLVMRR9 pKa = 11.84NIRR12 pKa = 11.84MEE14 pKa = 3.85PTANRR19 pKa = 11.84LQSRR23 pKa = 11.84VTIGFIVNSDD33 pKa = 2.9GRR35 pKa = 11.84IIGQRR40 pKa = 11.84VIKK43 pKa = 10.6NIDD46 pKa = 2.99GSTIAKK52 pKa = 9.36QMLVAISKK60 pKa = 9.95IKK62 pKa = 9.41WKK64 pKa = 10.03PGYY67 pKa = 9.59CNKK70 pKa = 10.18NPVPVYY76 pKa = 9.39LTIHH80 pKa = 6.09FNLRR84 pKa = 11.84LDD86 pKa = 3.43

Molecular weight: 9.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4304

0

4304

1505703

30

7558

349.8

39.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.966 ± 0.041

0.826 ± 0.016

5.29 ± 0.029

5.931 ± 0.046

4.97 ± 0.035

6.698 ± 0.04

1.707 ± 0.019

7.407 ± 0.04

7.37 ± 0.057

9.275 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.235 ± 0.024

5.952 ± 0.038

3.656 ± 0.029

3.578 ± 0.023

3.607 ± 0.028

6.684 ± 0.036

5.99 ± 0.082

6.483 ± 0.04

1.164 ± 0.012

4.212 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski