Clostridium autoethanogenum DSM 10061

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; Clostridium autoethanogenum

Average proteome isoelectric point is 6.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4013 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U5RZJ5|U5RZJ5_9CLOT 7 8-dihydroneopterin aldolase OS=Clostridium autoethanogenum DSM 10061 OX=1341692 GN=CAETHG_2732 PE=3 SV=1
MM1 pKa = 7.04ITVADD6 pKa = 3.98VVTQINTMLTTKK18 pKa = 10.28FPSTNITFTDD28 pKa = 3.48NPDD31 pKa = 2.96GHH33 pKa = 6.23EE34 pKa = 3.73RR35 pKa = 11.84AAFYY39 pKa = 7.69TTILSNASNQLSDD52 pKa = 4.03RR53 pKa = 11.84RR54 pKa = 11.84TQKK57 pKa = 10.85KK58 pKa = 9.66IGIKK62 pKa = 9.67IYY64 pKa = 9.88YY65 pKa = 9.01FPSDD69 pKa = 3.34EE70 pKa = 4.03YY71 pKa = 11.5DD72 pKa = 3.7YY73 pKa = 11.78LEE75 pKa = 4.47EE76 pKa = 4.74LCNIQNDD83 pKa = 3.44IDD85 pKa = 3.89EE86 pKa = 5.08LFCQGFPLQDD96 pKa = 2.78IYY98 pKa = 11.4INLDD102 pKa = 3.18EE103 pKa = 6.62DD104 pKa = 4.79GIDD107 pKa = 3.6YY108 pKa = 10.93NITDD112 pKa = 5.79GILQILFYY120 pKa = 10.72VNYY123 pKa = 9.83YY124 pKa = 9.75IEE126 pKa = 5.76RR127 pKa = 11.84DD128 pKa = 3.71TEE130 pKa = 4.19EE131 pKa = 5.49DD132 pKa = 3.3NLDD135 pKa = 3.91YY136 pKa = 11.04IEE138 pKa = 5.13NIEE141 pKa = 5.09DD142 pKa = 3.57EE143 pKa = 4.78EE144 pKa = 4.47NYY146 pKa = 10.32KK147 pKa = 10.81

Molecular weight: 17.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U5RYD7|U5RYD7_9CLOT Metallophosphoesterase OS=Clostridium autoethanogenum DSM 10061 OX=1341692 GN=CAETHG_3542 PE=4 SV=1
MM1 pKa = 7.45WMTYY5 pKa = 7.15QPKK8 pKa = 9.57KK9 pKa = 8.72KK10 pKa = 9.51QRR12 pKa = 11.84KK13 pKa = 8.04RR14 pKa = 11.84EE15 pKa = 3.69HH16 pKa = 6.25GFRR19 pKa = 11.84KK20 pKa = 9.97RR21 pKa = 11.84MRR23 pKa = 11.84TLSGRR28 pKa = 11.84NVIRR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.02GRR39 pKa = 11.84KK40 pKa = 8.99RR41 pKa = 11.84LTAA44 pKa = 4.18

Molecular weight: 5.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4013

0

4013

1212424

30

2878

302.1

33.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.771 ± 0.045

1.396 ± 0.017

5.461 ± 0.032

6.597 ± 0.044

4.357 ± 0.03

6.5 ± 0.04

1.349 ± 0.012

9.711 ± 0.047

9.31 ± 0.042

8.793 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.753 ± 0.018

6.218 ± 0.04

2.915 ± 0.023

2.469 ± 0.023

3.235 ± 0.025

6.775 ± 0.033

4.955 ± 0.04

6.684 ± 0.036

0.695 ± 0.011

4.058 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski