Bovine alphaherpesvirus 1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes; Herpesvirales; Herpesviridae; Alphaherpesvirinae; Varicellovirus

Average proteome isoelectric point is 7.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 71 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q76PK0|Q76PK0_9ALPH Capsid scaffolding protein OS=Bovine alphaherpesvirus 1 OX=10320 GN=UL26 PE=3 SV=1
MM1 pKa = 7.36PAARR5 pKa = 11.84TGTLAAVALILLCGAAVLGRR25 pKa = 11.84PAPDD29 pKa = 4.02DD30 pKa = 3.8LCFADD35 pKa = 4.08VRR37 pKa = 11.84RR38 pKa = 11.84TGMAPSRR45 pKa = 11.84PLGPVLNLAASDD57 pKa = 3.54LTSRR61 pKa = 11.84VSVRR65 pKa = 11.84AVDD68 pKa = 3.92ASRR71 pKa = 11.84GCALALLDD79 pKa = 3.67MAEE82 pKa = 4.3TVVPGGPRR90 pKa = 11.84AADD93 pKa = 3.39VVDD96 pKa = 4.23VGWAYY101 pKa = 11.0QDD103 pKa = 3.56GDD105 pKa = 4.21CMVPLAYY112 pKa = 9.55RR113 pKa = 11.84QYY115 pKa = 10.87FNCTGGALPGQNVCAGLSEE134 pKa = 4.01TRR136 pKa = 11.84IRR138 pKa = 11.84GGFGTSDD145 pKa = 3.4YY146 pKa = 11.42ALYY149 pKa = 8.63GTSLVLRR156 pKa = 11.84PGLYY160 pKa = 10.4DD161 pKa = 3.67RR162 pKa = 11.84GTYY165 pKa = 9.46IYY167 pKa = 10.27FLGYY171 pKa = 10.52GPDD174 pKa = 4.19DD175 pKa = 3.88IYY177 pKa = 11.6VGSVTLMVGADD188 pKa = 3.09IHH190 pKa = 6.87KK191 pKa = 9.82YY192 pKa = 9.69PCGLDD197 pKa = 2.98RR198 pKa = 11.84GLGVALHH205 pKa = 6.47HH206 pKa = 6.78KK207 pKa = 10.03SGPARR212 pKa = 11.84PLTEE216 pKa = 5.8DD217 pKa = 4.34DD218 pKa = 4.19ATGDD222 pKa = 3.7WACGCFPALVEE233 pKa = 4.6VDD235 pKa = 4.14AVWGNVSAAEE245 pKa = 4.6LGLADD250 pKa = 5.37PIDD253 pKa = 4.03YY254 pKa = 10.57ADD256 pKa = 3.77EE257 pKa = 4.71GGEE260 pKa = 4.19VEE262 pKa = 4.59VLEE265 pKa = 5.2DD266 pKa = 3.65EE267 pKa = 5.03AGSASGNLPQDD278 pKa = 4.52DD279 pKa = 5.17PDD281 pKa = 5.43PDD283 pKa = 4.12LADD286 pKa = 3.86CRR288 pKa = 11.84TVGLFSEE295 pKa = 4.51SDD297 pKa = 3.42MFRR300 pKa = 11.84TASGPEE306 pKa = 3.85SLLIGAVAKK315 pKa = 10.63DD316 pKa = 3.9VLTVPLNLPPGRR328 pKa = 11.84SYY330 pKa = 11.24EE331 pKa = 4.08ALRR334 pKa = 11.84NASLEE339 pKa = 4.36CNSRR343 pKa = 11.84PRR345 pKa = 11.84EE346 pKa = 4.01TGDD349 pKa = 3.21AAVVVMSLQEE359 pKa = 3.81PARR362 pKa = 11.84LEE364 pKa = 3.95RR365 pKa = 11.84RR366 pKa = 11.84PDD368 pKa = 3.2ARR370 pKa = 11.84ATDD373 pKa = 3.88PEE375 pKa = 4.53FGLFGLPDD383 pKa = 4.19DD384 pKa = 4.18PAVRR388 pKa = 11.84RR389 pKa = 11.84GILIGLAIALLVLLFSLVIVLVCACRR415 pKa = 11.84LARR418 pKa = 11.84AAKK421 pKa = 8.82AARR424 pKa = 11.84RR425 pKa = 11.84ARR427 pKa = 11.84AATFAKK433 pKa = 10.28SNPAYY438 pKa = 10.29EE439 pKa = 4.49PMLSVV444 pKa = 3.74

Molecular weight: 46.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q65546|Q65546_9ALPH Protein V57 OS=Bovine alphaherpesvirus 1 OX=10320 GN=UL3.5 PE=4 SV=1
MM1 pKa = 7.51RR2 pKa = 11.84RR3 pKa = 11.84VEE5 pKa = 4.23PVLSLLAAGGWQVSAAAAASASAPADD31 pKa = 3.84DD32 pKa = 5.17GEE34 pKa = 4.57RR35 pKa = 11.84SSGPALAGPGLAAAGLGPNPDD56 pKa = 3.31CVAFDD61 pKa = 3.83TLFMVSSIDD70 pKa = 3.47EE71 pKa = 4.18LGRR74 pKa = 11.84RR75 pKa = 11.84QLTDD79 pKa = 3.94TIRR82 pKa = 11.84KK83 pKa = 8.84DD84 pKa = 4.08LKK86 pKa = 10.15RR87 pKa = 11.84TLAKK91 pKa = 9.56YY92 pKa = 8.7TIACTKK98 pKa = 9.43TSSFSGAQARR108 pKa = 11.84GLSARR113 pKa = 11.84AARR116 pKa = 11.84AAARR120 pKa = 11.84GLRR123 pKa = 11.84SNKK126 pKa = 9.3SLQMFLLCQRR136 pKa = 11.84AHH138 pKa = 5.86AQRR141 pKa = 11.84VRR143 pKa = 11.84DD144 pKa = 3.71QLHH147 pKa = 6.1SVIQSRR153 pKa = 11.84KK154 pKa = 6.64PRR156 pKa = 11.84KK157 pKa = 8.98YY158 pKa = 8.55YY159 pKa = 9.93TRR161 pKa = 11.84SSDD164 pKa = 2.86GGTRR168 pKa = 11.84PAVPVYY174 pKa = 10.58VYY176 pKa = 9.53EE177 pKa = 4.82FSAAGPVYY185 pKa = 10.26IHH187 pKa = 7.5RR188 pKa = 11.84DD189 pKa = 3.24NTLGPDD195 pKa = 3.67SRR197 pKa = 11.84PGEE200 pKa = 4.06QPHH203 pKa = 6.23RR204 pKa = 4.12

Molecular weight: 21.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

69

2

71

38093

87

3291

536.5

56.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

19.153 ± 0.739

1.848 ± 0.133

5.046 ± 0.139

5.321 ± 0.173

3.137 ± 0.239

9.044 ± 0.367

1.869 ± 0.126

1.88 ± 0.112

1.328 ± 0.14

9.815 ± 0.289

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.467 ± 0.104

1.683 ± 0.159

7.807 ± 0.536

2.197 ± 0.103

9.521 ± 0.368

5.119 ± 0.181

3.906 ± 0.169

6.573 ± 0.261

0.948 ± 0.052

2.336 ± 0.188
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski