Wigeon coronavirus HKU20

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Deltacoronavirus; Andecovirus

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H9BR31|H9BR31_9NIDO NS7b protein OS=Wigeon coronavirus HKU20 OX=1159908 GN=NS7b PE=4 SV=1
MM1 pKa = 7.35SLVIDD6 pKa = 4.5GDD8 pKa = 4.04PLQVADD14 pKa = 4.49VLRR17 pKa = 11.84QLSLIFHH24 pKa = 6.14TPIYY28 pKa = 10.43DD29 pKa = 3.62CNLRR33 pKa = 11.84LHH35 pKa = 5.78WVEE38 pKa = 5.34GVPCTLSFCEE48 pKa = 4.08LCYY51 pKa = 11.12DD52 pKa = 4.22FLQPIQADD60 pKa = 3.64SPGFSDD66 pKa = 5.2SSTSPDD72 pKa = 3.47FVDD75 pKa = 3.75SQQ77 pKa = 3.64

Molecular weight: 8.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H9BR30|H9BR30_9NIDO NS7a protein OS=Wigeon coronavirus HKU20 OX=1159908 GN=NS7a PE=4 SV=1
MM1 pKa = 7.65AVPSVPKK8 pKa = 10.31ADD10 pKa = 4.02CSWFQIIKK18 pKa = 9.74AQNKK22 pKa = 8.62KK23 pKa = 9.03VQPLAFKK30 pKa = 11.01GDD32 pKa = 3.7GVPINTTLPKK42 pKa = 10.19EE43 pKa = 4.09MCHH46 pKa = 6.72GYY48 pKa = 7.94WLRR51 pKa = 11.84YY52 pKa = 9.55KK53 pKa = 10.2RR54 pKa = 11.84VKK56 pKa = 10.32PGGAPLPPSYY66 pKa = 10.98SFYY69 pKa = 10.38YY70 pKa = 9.98TGTGPRR76 pKa = 11.84ATLKK80 pKa = 10.9YY81 pKa = 10.97GEE83 pKa = 4.99LQDD86 pKa = 5.8SDD88 pKa = 3.91NQDD91 pKa = 2.9NVNRR95 pKa = 11.84VLWVKK100 pKa = 10.85SPDD103 pKa = 3.43ADD105 pKa = 3.57TSIKK109 pKa = 10.42PKK111 pKa = 9.23VAKK114 pKa = 10.22RR115 pKa = 11.84NPDD118 pKa = 3.19KK119 pKa = 11.25HH120 pKa = 6.39PLLPLRR126 pKa = 11.84FKK128 pKa = 10.93PGDD131 pKa = 3.56GPVQGFRR138 pKa = 11.84IDD140 pKa = 3.65PFQSRR145 pKa = 11.84GRR147 pKa = 11.84SVEE150 pKa = 4.02RR151 pKa = 11.84GQQDD155 pKa = 2.74RR156 pKa = 11.84RR157 pKa = 11.84AQSAEE162 pKa = 3.67PQRR165 pKa = 11.84PKK167 pKa = 10.02QRR169 pKa = 11.84EE170 pKa = 3.83RR171 pKa = 11.84SRR173 pKa = 11.84SAPSRR178 pKa = 11.84AAPKK182 pKa = 9.68QHH184 pKa = 7.29AIPKK188 pKa = 9.73RR189 pKa = 11.84VLLKK193 pKa = 10.81GKK195 pKa = 8.28TISGTFGKK203 pKa = 10.44RR204 pKa = 11.84SPAKK208 pKa = 10.52ANVGSPDD215 pKa = 3.57TEE217 pKa = 4.07KK218 pKa = 10.41TGLGDD223 pKa = 3.84PRR225 pKa = 11.84LMALMRR231 pKa = 11.84FVPGTQEE238 pKa = 3.95LLFAGHH244 pKa = 7.68LKK246 pKa = 10.6HH247 pKa = 6.84KK248 pKa = 7.01MQPEE252 pKa = 4.91GVQLTFTYY260 pKa = 10.56DD261 pKa = 2.96ITVKK265 pKa = 10.4RR266 pKa = 11.84DD267 pKa = 3.12NKK269 pKa = 10.68DD270 pKa = 2.6FDD272 pKa = 5.24RR273 pKa = 11.84IVEE276 pKa = 4.13ALNSVVDD283 pKa = 3.63MVYY286 pKa = 10.43EE287 pKa = 3.89PEE289 pKa = 4.14GEE291 pKa = 3.95IRR293 pKa = 11.84KK294 pKa = 8.97DD295 pKa = 3.46QQSKK299 pKa = 11.15KK300 pKa = 9.59DD301 pKa = 3.58TQPKK305 pKa = 9.62KK306 pKa = 10.07KK307 pKa = 10.21AEE309 pKa = 4.3KK310 pKa = 8.91KK311 pKa = 9.19TPTNPQKK318 pKa = 10.9QPDD321 pKa = 4.11KK322 pKa = 10.48PVATIVDD329 pKa = 3.74NGDD332 pKa = 3.44SQVINWGTDD341 pKa = 3.19PAEE344 pKa = 4.29GLEE347 pKa = 4.34SFSS350 pKa = 4.03

Molecular weight: 39.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

8473

66

6206

847.3

94.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.07 ± 0.297

2.655 ± 0.338

5.618 ± 0.469

3.446 ± 0.239

4.473 ± 0.368

5.512 ± 0.479

2.16 ± 0.379

5.996 ± 0.476

4.851 ± 0.95

9.465 ± 0.707

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.865 ± 0.103

5.417 ± 0.865

4.603 ± 0.966

3.836 ± 0.636

3.493 ± 0.525

6.763 ± 0.583

7.223 ± 0.687

9.418 ± 0.712

0.956 ± 0.287

5.181 ± 0.638
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski