Hubei tombus-like virus 43

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KGM0|A0A1L3KGM0_9VIRU Capsid protein OS=Hubei tombus-like virus 43 OX=1923291 PE=3 SV=1
MM1 pKa = 7.8PNKK4 pKa = 8.11QTKK7 pKa = 8.55RR8 pKa = 11.84TTKK11 pKa = 8.44NTKK14 pKa = 9.46PKK16 pKa = 8.15QQRR19 pKa = 11.84RR20 pKa = 11.84KK21 pKa = 9.03QPKK24 pKa = 7.37AQRR27 pKa = 11.84NVVSTQSAPVSRR39 pKa = 11.84GHH41 pKa = 7.29RR42 pKa = 11.84IKK44 pKa = 9.81QTAPKK49 pKa = 8.53ITASSKK55 pKa = 9.93GVNVVHH61 pKa = 6.79SEE63 pKa = 3.79ARR65 pKa = 11.84SVAVTSGSSTVDD77 pKa = 3.19MSWTLLPQGSNFPWLAAMAIQFDD100 pKa = 4.17KK101 pKa = 11.07IVWNKK106 pKa = 9.84VSVTFVPIVGTNLGGDD122 pKa = 3.29VVMYY126 pKa = 10.25FDD128 pKa = 4.96TEE130 pKa = 4.22ADD132 pKa = 3.61DD133 pKa = 4.08TRR135 pKa = 11.84VTDD138 pKa = 3.67YY139 pKa = 11.36VSAYY143 pKa = 10.22SMEE146 pKa = 4.27GAVSGRR152 pKa = 11.84VWDD155 pKa = 3.65MCTLHH160 pKa = 6.98AKK162 pKa = 10.01HH163 pKa = 6.95ADD165 pKa = 3.17LHH167 pKa = 5.27NQKK170 pKa = 10.48SYY172 pKa = 10.21AAKK175 pKa = 10.71GEE177 pKa = 4.31TSKK180 pKa = 11.46SLIGSPGMFCMLATLQTAATANTIIGRR207 pKa = 11.84WVINYY212 pKa = 8.69DD213 pKa = 3.48VTFLKK218 pKa = 10.7PEE220 pKa = 3.95AVTASSLLSLAGEE233 pKa = 4.78LDD235 pKa = 3.57PTAMRR240 pKa = 11.84TYY242 pKa = 10.54IEE244 pKa = 4.32AHH246 pKa = 4.65WTDD249 pKa = 4.52YY250 pKa = 8.8YY251 pKa = 10.9THH253 pKa = 7.19AACVSNCPCYY263 pKa = 10.16TSGDD267 pKa = 3.63QHH269 pKa = 5.22EE270 pKa = 4.41QCYY273 pKa = 10.4EE274 pKa = 3.79EE275 pKa = 4.84ALVGGMCLVDD285 pKa = 5.23KK286 pKa = 10.76VTDD289 pKa = 3.65TCSIMCPCNSLKK301 pKa = 10.6NASLGHH307 pKa = 5.62KK308 pKa = 10.17HH309 pKa = 6.1MIGHH313 pKa = 5.91SKK315 pKa = 10.81DD316 pKa = 3.8LIEE319 pKa = 4.4MDD321 pKa = 3.85KK322 pKa = 8.37TTHH325 pKa = 6.6DD326 pKa = 4.33AEE328 pKa = 4.31PMEE331 pKa = 4.53VDD333 pKa = 4.35DD334 pKa = 6.29LVAINQQPTLADD346 pKa = 3.58EE347 pKa = 4.28LTSRR351 pKa = 11.84GWVLNLPVDD360 pKa = 4.44NNDD363 pKa = 3.18EE364 pKa = 4.0MAKK367 pKa = 9.65FLKK370 pKa = 10.8AIDD373 pKa = 4.74IIDD376 pKa = 3.68KK377 pKa = 8.31VTSVRR382 pKa = 11.84EE383 pKa = 4.27GDD385 pKa = 3.05HH386 pKa = 6.83HH387 pKa = 6.2MWNGQTIHH395 pKa = 6.95ASSTSSSDD403 pKa = 3.5SEE405 pKa = 4.0MDD407 pKa = 3.08

Molecular weight: 44.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KGM0|A0A1L3KGM0_9VIRU Capsid protein OS=Hubei tombus-like virus 43 OX=1923291 PE=3 SV=1
MM1 pKa = 7.73NIFTPARR8 pKa = 11.84FFFKK12 pKa = 9.75ITCSTKK18 pKa = 10.05KK19 pKa = 9.83RR20 pKa = 11.84ALVSFLVLAASLAAFVGFLILLLAFQHH47 pKa = 5.73VDD49 pKa = 3.26YY50 pKa = 11.52VPIVDD55 pKa = 3.78VNSARR60 pKa = 11.84WIEE63 pKa = 4.14QQRR66 pKa = 11.84VTYY69 pKa = 10.3AIRR72 pKa = 11.84SYY74 pKa = 11.53SLIEE78 pKa = 3.93SLVILFTSILNRR90 pKa = 11.84LDD92 pKa = 3.39LTINMQPAIHH102 pKa = 6.57ILSPTMHH109 pKa = 6.22QMIITQRR116 pKa = 11.84EE117 pKa = 3.8QCDD120 pKa = 3.66YY121 pKa = 10.48WDD123 pKa = 3.61TALRR127 pKa = 11.84FYY129 pKa = 11.05DD130 pKa = 3.51EE131 pKa = 4.83HH132 pKa = 7.86LKK134 pKa = 10.69QFDD137 pKa = 3.78DD138 pKa = 4.02AGSYY142 pKa = 10.85SAMFVLTGTQIAGLAHH158 pKa = 6.38SEE160 pKa = 4.21SNIYY164 pKa = 10.29CRR166 pKa = 11.84GLSRR170 pKa = 11.84LGYY173 pKa = 10.22ASLPRR178 pKa = 11.84AYY180 pKa = 9.28PKK182 pKa = 10.42SWLRR186 pKa = 11.84RR187 pKa = 11.84IDD189 pKa = 4.65LINSEE194 pKa = 3.76QRR196 pKa = 11.84FYY198 pKa = 11.91NKK200 pKa = 9.55IAMRR204 pKa = 11.84LDD206 pKa = 3.89MIYY209 pKa = 10.5QLADD213 pKa = 4.08EE214 pKa = 5.38IIVGVLDD221 pKa = 4.11NIMWVLRR228 pKa = 11.84HH229 pKa = 6.21PFLSTFVVLLGLFVMMAIGFYY250 pKa = 10.73LSTLYY255 pKa = 10.49HH256 pKa = 5.9IQWSRR261 pKa = 11.84KK262 pKa = 8.34IKK264 pKa = 10.08NCAFAIRR271 pKa = 11.84QSAPLLPFRR280 pKa = 11.84PGVVKK285 pKa = 10.71DD286 pKa = 3.38VLKK289 pKa = 10.94RR290 pKa = 11.84RR291 pKa = 11.84LKK293 pKa = 9.41PLKK296 pKa = 9.21VTPTPDD302 pKa = 2.61GHH304 pKa = 8.45LCMAEE309 pKa = 3.84VRR311 pKa = 11.84RR312 pKa = 11.84NMEE315 pKa = 3.78RR316 pKa = 11.84TCIGFLLEE324 pKa = 3.97YY325 pKa = 9.75FKK327 pKa = 10.59QVRR330 pKa = 11.84DD331 pKa = 3.39IGGSPTRR338 pKa = 11.84NSHH341 pKa = 4.75YY342 pKa = 10.5GRR344 pKa = 11.84RR345 pKa = 11.84LHH347 pKa = 6.69ICCPDD352 pKa = 3.74LTAIDD357 pKa = 3.87HH358 pKa = 6.95DD359 pKa = 4.17KK360 pKa = 11.63VNIANYY366 pKa = 10.04KK367 pKa = 10.56NDD369 pKa = 3.79VQCHH373 pKa = 5.08KK374 pKa = 11.15SIDD377 pKa = 3.93CNVAKK382 pKa = 10.14QIPAAIMSYY391 pKa = 11.04VDD393 pKa = 4.52FHH395 pKa = 8.76IPIHH399 pKa = 6.48EE400 pKa = 4.47IADD403 pKa = 3.95SVTGPTLVITHH414 pKa = 6.44EE415 pKa = 4.58FKK417 pKa = 11.03EE418 pKa = 4.56GMDD421 pKa = 3.77EE422 pKa = 3.87KK423 pKa = 10.65WFDD426 pKa = 3.82GEE428 pKa = 4.59CTVKK432 pKa = 10.95ASGGLVQMSVRR443 pKa = 11.84GGNEE447 pKa = 3.41YY448 pKa = 10.67SHH450 pKa = 7.02YY451 pKa = 9.18YY452 pKa = 9.91HH453 pKa = 7.47DD454 pKa = 4.11WKK456 pKa = 11.23DD457 pKa = 3.32EE458 pKa = 3.79GVIIGKK464 pKa = 8.38SHH466 pKa = 7.35AINYY470 pKa = 9.25IKK472 pKa = 9.91VAKK475 pKa = 9.01YY476 pKa = 8.09EE477 pKa = 3.92HH478 pKa = 6.99SIVILLWPVTGTFTRR493 pKa = 11.84YY494 pKa = 10.19DD495 pKa = 3.61PNALFDD501 pKa = 3.93LRR503 pKa = 11.84YY504 pKa = 10.58DD505 pKa = 4.31EE506 pKa = 5.68ISLPDD511 pKa = 3.7GSSVVNTGKK520 pKa = 9.96HH521 pKa = 5.58FEE523 pKa = 4.41VKK525 pKa = 10.67DD526 pKa = 3.59NMGGHH531 pKa = 7.47LGLIPVDD538 pKa = 3.87TIVRR542 pKa = 11.84TAFTAKK548 pKa = 9.95GMARR552 pKa = 11.84DD553 pKa = 3.78PKK555 pKa = 10.84YY556 pKa = 10.61VANVDD561 pKa = 3.16ALLRR565 pKa = 11.84ARR567 pKa = 11.84FTQDD571 pKa = 2.59KK572 pKa = 10.4CDD574 pKa = 3.56MSLLPQASLVTYY586 pKa = 10.41RR587 pKa = 11.84LADD590 pKa = 4.39DD591 pKa = 4.34YY592 pKa = 11.47SLTMNHH598 pKa = 6.02MSSVIDD604 pKa = 4.09GPVVGLTTMQRR615 pKa = 11.84LRR617 pKa = 11.84CATLTLIKK625 pKa = 10.22GWTPRR630 pKa = 11.84RR631 pKa = 11.84FMHH634 pKa = 7.05LYY636 pKa = 10.46ASLLRR641 pKa = 11.84WCIGSDD647 pKa = 3.79QDD649 pKa = 5.26SSWLPWLWTDD659 pKa = 4.14HH660 pKa = 6.01VAPNYY665 pKa = 9.64EE666 pKa = 4.42VYY668 pKa = 10.26TPGSLSLMEE677 pKa = 4.51IKK679 pKa = 10.33QNKK682 pKa = 8.21QMLNAQPFQLPGQSCSTHH700 pKa = 5.71SAPNNEE706 pKa = 4.34CAAVKK711 pKa = 9.92HH712 pKa = 5.09VRR714 pKa = 11.84KK715 pKa = 8.55PTVKK719 pKa = 9.84RR720 pKa = 11.84VKK722 pKa = 10.35EE723 pKa = 4.09STKK726 pKa = 10.76DD727 pKa = 3.26QTPATSSTHH736 pKa = 6.25RR737 pKa = 11.84KK738 pKa = 7.98PSRR741 pKa = 11.84GEE743 pKa = 3.82RR744 pKa = 11.84KK745 pKa = 9.45NDD747 pKa = 2.88RR748 pKa = 11.84SAAAVAPEE756 pKa = 3.95PTQRR760 pKa = 11.84LGPSRR765 pKa = 11.84RR766 pKa = 11.84IPDD769 pKa = 3.3RR770 pKa = 11.84AVPNAGRR777 pKa = 11.84RR778 pKa = 11.84HH779 pKa = 4.42QQSGGRR785 pKa = 11.84NDD787 pKa = 4.18ASGKK791 pKa = 8.18STADD795 pKa = 3.3RR796 pKa = 11.84RR797 pKa = 11.84PDD799 pKa = 3.01RR800 pKa = 11.84RR801 pKa = 11.84NADD804 pKa = 3.32KK805 pKa = 11.36VNTRR809 pKa = 11.84TTPALNDD816 pKa = 3.07RR817 pKa = 11.84GMGKK821 pKa = 9.71KK822 pKa = 10.36VSGKK826 pKa = 8.3STSRR830 pKa = 11.84PNSSPRR836 pKa = 11.84KK837 pKa = 8.03SKK839 pKa = 10.95SVVHH843 pKa = 6.6AKK845 pKa = 9.89EE846 pKa = 3.81LQQRR850 pKa = 11.84PNIPEE855 pKa = 3.44IRR857 pKa = 11.84NVDD860 pKa = 3.35KK861 pKa = 11.35SDD863 pKa = 3.33GPKK866 pKa = 9.68EE867 pKa = 4.08HH868 pKa = 6.75NPEE871 pKa = 3.99STRR874 pKa = 11.84VPFDD878 pKa = 3.25FRR880 pKa = 11.84AFCEE884 pKa = 4.16QYY886 pKa = 10.33RR887 pKa = 11.84EE888 pKa = 4.47CNPPPPIPCEE898 pKa = 3.83RR899 pKa = 11.84QEE901 pKa = 4.15QLGPRR906 pKa = 11.84SEE908 pKa = 4.88NIPEE912 pKa = 4.17IKK914 pKa = 10.02QLPSVCGDD922 pKa = 3.72GLQQ925 pKa = 3.7

Molecular weight: 104.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1874

407

925

624.7

70.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.937 ± 0.548

2.081 ± 0.167

5.923 ± 0.323

4.269 ± 0.273

3.308 ± 0.515

4.909 ± 0.446

3.308 ± 0.123

6.03 ± 0.528

5.39 ± 0.275

8.645 ± 0.932

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.775 ± 0.485

4.482 ± 0.192

5.923 ± 0.662

4.055 ± 0.07

6.03 ± 1.064

7.737 ± 0.442

6.724 ± 0.858

6.83 ± 0.416

1.708 ± 0.186

2.935 ± 0.261
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski