Candidatus Marinamargulisbacteria bacterium SCGC AG-343-K17

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Candidatus Margulisbacteria; Candidatus Marinamargulisbacteria

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 504 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A328QZW2|A0A328QZW2_9BACT Uncharacterized protein OS=Candidatus Marinamargulisbacteria bacterium SCGC AG-343-K17 OX=2184341 GN=DID73_01230 PE=4 SV=1
MM1 pKa = 7.63KK2 pKa = 10.21NNAMIPNMEE11 pKa = 4.71INNQQTTVDD20 pKa = 3.19ISLDD24 pKa = 3.15IEE26 pKa = 4.74SYY28 pKa = 11.1VITLCTHH35 pKa = 6.86LGLQFDD41 pKa = 4.13HH42 pKa = 8.27LEE44 pKa = 3.76ITLLSKK50 pKa = 10.57EE51 pKa = 5.08AISKK55 pKa = 9.82MNLEE59 pKa = 4.4YY60 pKa = 10.61FDD62 pKa = 6.31LDD64 pKa = 3.93TPTDD68 pKa = 3.79TISFNLTPEE77 pKa = 3.83AAITGDD83 pKa = 3.64IYY85 pKa = 11.25LCPDD89 pKa = 4.49IILANATTYY98 pKa = 11.07LSDD101 pKa = 3.84FNEE104 pKa = 3.9EE105 pKa = 3.91LKK107 pKa = 10.63IVLIHH112 pKa = 7.41SMLHH116 pKa = 5.33LMGYY120 pKa = 8.78TDD122 pKa = 4.98DD123 pKa = 3.84TPEE126 pKa = 4.59NYY128 pKa = 10.5SHH130 pKa = 6.89MKK132 pKa = 8.0NTQEE136 pKa = 5.25KK137 pKa = 9.33IYY139 pKa = 10.8QEE141 pKa = 4.41LSQQ144 pKa = 4.09

Molecular weight: 16.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A328QTK1|A0A328QTK1_9BACT Uncharacterized protein OS=Candidatus Marinamargulisbacteria bacterium SCGC AG-343-K17 OX=2184341 GN=DID73_00330 PE=4 SV=1
MM1 pKa = 7.13QKK3 pKa = 10.01IIRR6 pKa = 11.84SNQPNVVHH14 pKa = 6.95EE15 pKa = 4.54GRR17 pKa = 11.84LPRR20 pKa = 11.84QVQLSRR26 pKa = 11.84HH27 pKa = 5.37KK28 pKa = 10.59RR29 pKa = 11.84VTPPRR34 pKa = 11.84LSRR37 pKa = 11.84SVNGLPLNSTQYY49 pKa = 8.51WVKK52 pKa = 9.84TINYY56 pKa = 8.42QLDD59 pKa = 3.7EE60 pKa = 4.36TGVNIRR66 pKa = 11.84RR67 pKa = 11.84NPQGQPVLEE76 pKa = 4.42TTWTYY81 pKa = 10.92TRR83 pKa = 11.84GLWRR87 pKa = 11.84PGCTCVDD94 pKa = 3.51LRR96 pKa = 11.84DD97 pKa = 3.82PFLL100 pKa = 5.59

Molecular weight: 11.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

504

0

504

183195

59

3940

363.5

40.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.866 ± 0.1

1.096 ± 0.04

5.996 ± 0.096

5.503 ± 0.121

4.597 ± 0.078

6.157 ± 0.119

2.558 ± 0.06

8.196 ± 0.13

6.809 ± 0.145

9.66 ± 0.112

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.461 ± 0.047

5.629 ± 0.09

3.856 ± 0.073

4.361 ± 0.093

3.486 ± 0.07

7.657 ± 0.135

5.834 ± 0.1

6.143 ± 0.115

0.888 ± 0.035

3.246 ± 0.065
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski