Olavius sp. associated proteobacterium Delta 1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; unclassified Deltaproteobacteria

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10776 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6J4XFR5|A0A6J4XFR5_9DELT Glycine betaine ABC transport system glycine betaine-binding protein OpuAC OS=Olavius sp. associated proteobacterium Delta 1 OX=698986 GN=D1BOALGB6SA_8809 PE=4 SV=1
YYY2 pKa = 9.75YYY4 pKa = 10.64FDDD7 pKa = 5.8DD8 pKa = 4.68GDDD11 pKa = 3.64LYYY14 pKa = 10.96RR15 pKa = 11.84EEE17 pKa = 4.13PDDD20 pKa = 4.17DD21 pKa = 5.34RR22 pKa = 11.84MQWKKK27 pKa = 8.86QWAGYYY33 pKa = 8.28QLYYY37 pKa = 10.01YY38 pKa = 10.96DD39 pKa = 4.04AVDDD43 pKa = 3.78YY44 pKa = 9.95EE45 pKa = 4.16WPNQVVNDDD54 pKa = 3.97ALTLDDD60 pKa = 4.29ADDD63 pKa = 4.73DDD65 pKa = 4.55PRR67 pKa = 11.84SHHH70 pKa = 7.21MDDD73 pKa = 3.76ATTFDDD79 pKa = 5.08NATYYY84 pKa = 9.85HH85 pKa = 5.8FTSDDD90 pKa = 2.84GAIIVDDD97 pKa = 3.95DD98 pKa = 3.95DDD100 pKa = 4.57EEE102 pKa = 4.42SKKK105 pKa = 9.97PTGVGEEE112 pKa = 4.11TLLSTGFEEE121 pKa = 4.21EE122 pKa = 6.72SPAWQAHHH130 pKa = 4.57DDD132 pKa = 3.99NGAPDDD138 pKa = 3.27TRR140 pKa = 11.84ATDDD144 pKa = 3.6DDD146 pKa = 4.5HH147 pKa = 6.39GSWSAKKK154 pKa = 9.54DDD156 pKa = 3.41TNQGNLTSDDD166 pKa = 3.4LDDD169 pKa = 3.73GDDD172 pKa = 4.16VAANGDDD179 pKa = 4.29ITVDDD184 pKa = 3.7WFRR187 pKa = 11.84KKK189 pKa = 10.01DD190 pKa = 4.87DD191 pKa = 3.78EEE193 pKa = 5.96DD194 pKa = 3.9DD195 pKa = 4.01EE196 pKa = 5.58ILYYY200 pKa = 10.53YY201 pKa = 10.46YY202 pKa = 10.82DD203 pKa = 3.55ANYYY207 pKa = 10.12DD208 pKa = 4.76IMDDD212 pKa = 5.0EEE214 pKa = 5.55DD215 pKa = 5.03YYY217 pKa = 9.72DDD219 pKa = 3.28RR220 pKa = 11.84DDD222 pKa = 3.73DD223 pKa = 3.29WIHHH227 pKa = 4.64YY228 pKa = 10.13EEE230 pKa = 5.11ITDDD234 pKa = 3.7DD235 pKa = 3.73YYY237 pKa = 10.02TSNFRR242 pKa = 11.84IRR244 pKa = 11.84FYYY247 pKa = 11.1HH248 pKa = 6.47KK249 pKa = 9.73TSTRR253 pKa = 11.84EEE255 pKa = 4.01VWIDDD260 pKa = 3.31DD261 pKa = 4.79EEE263 pKa = 4.16TKKK266 pKa = 10.81GGGTGGWEEE275 pKa = 3.94EE276 pKa = 4.24TGSDDD281 pKa = 3.09AYYY284 pKa = 10.76GDDD287 pKa = 4.07YY288 pKa = 9.58WTGADDD294 pKa = 3.47DDD296 pKa = 4.56YY297 pKa = 10.16ATWTPDDD304 pKa = 2.9PEEE307 pKa = 5.3DD308 pKa = 3.7EE309 pKa = 5.33DD310 pKa = 4.51DDD312 pKa = 4.18YY313 pKa = 11.37DD314 pKa = 5.2YYY316 pKa = 11.56RR317 pKa = 11.84WHHH320 pKa = 5.52

Molecular weight: 36.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6J4XFE4|A0A6J4XFE4_9DELT Uncharacterized protein OS=Olavius sp. associated proteobacterium Delta 1 OX=698986 GN=D1BOALGB6SA_7237 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSRR9 pKa = 11.84IKK11 pKa = 10.45RR12 pKa = 11.84ARR14 pKa = 11.84NHH16 pKa = 6.07GFLKK20 pKa = 10.62RR21 pKa = 11.84MSTKK25 pKa = 9.49QGRR28 pKa = 11.84RR29 pKa = 11.84IINRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.3GRR39 pKa = 11.84ARR41 pKa = 11.84LAGG44 pKa = 3.63

Molecular weight: 5.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10776

0

10776

2777551

20

14640

257.8

28.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.26 ± 0.027

1.225 ± 0.014

5.568 ± 0.027

5.994 ± 0.025

4.477 ± 0.017

7.339 ± 0.027

2.05 ± 0.012

7.291 ± 0.028

5.832 ± 0.036

9.874 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.623 ± 0.016

3.969 ± 0.018

4.39 ± 0.016

3.626 ± 0.014

5.391 ± 0.022

5.953 ± 0.019

5.035 ± 0.022

6.64 ± 0.023

1.23 ± 0.01

3.235 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski