Oryza nivara (Indian wild rice) (Oryza sativa f. spontanea)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales; Poaceae; BOP clade; Oryzoideae; Oryzeae;

Average proteome isoelectric point is 6.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 46176 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0E0IHB6|A0A0E0IHB6_ORYNI Isoform of A0A0E0IHB5 Uncharacterized protein OS=Oryza nivara OX=4536 PE=4 SV=1
MM1 pKa = 7.95DD2 pKa = 4.96GVDD5 pKa = 5.1DD6 pKa = 5.24GLICSTSCYY15 pKa = 10.16KK16 pKa = 10.0MCGHH20 pKa = 7.43ADD22 pKa = 3.17KK23 pKa = 11.3SSIIDD28 pKa = 4.26GEE30 pKa = 4.3IGWPSMEE37 pKa = 4.71ISPCSTPYY45 pKa = 9.15GTPLFSRR52 pKa = 11.84EE53 pKa = 4.13SSCSSFASCFSSLDD67 pKa = 3.68DD68 pKa = 4.04YY69 pKa = 11.74LVEE72 pKa = 4.24TDD74 pKa = 3.63SEE76 pKa = 4.47EE77 pKa = 4.28EE78 pKa = 4.15IEE80 pKa = 5.05VLDD83 pKa = 4.09TGQLHH88 pKa = 6.57PGILFSDD95 pKa = 3.83EE96 pKa = 4.47SMEE99 pKa = 4.07QRR101 pKa = 11.84EE102 pKa = 4.13ADD104 pKa = 3.44SVQVEE109 pKa = 5.15EE110 pKa = 4.52YY111 pKa = 9.96QVSHH115 pKa = 6.15SAVVNDD121 pKa = 4.95DD122 pKa = 3.64SSISIPTNQNNSSGQLQLEE141 pKa = 4.18IHH143 pKa = 6.92LDD145 pKa = 3.38ATNEE149 pKa = 3.79KK150 pKa = 9.98SVPSNAILDD159 pKa = 4.05ANVTDD164 pKa = 3.78PHH166 pKa = 7.84QEE168 pKa = 4.02VISNGGLIEE177 pKa = 4.19ACYY180 pKa = 9.82GVPVDD185 pKa = 6.53DD186 pKa = 5.97IDD188 pKa = 5.13LKK190 pKa = 11.05QSNILDD196 pKa = 3.97GEE198 pKa = 4.73EE199 pKa = 3.99ITSLPMADD207 pKa = 4.82NEE209 pKa = 4.34MTPLDD214 pKa = 3.98DD215 pKa = 3.7MKK217 pKa = 10.5EE218 pKa = 3.85ISSIVYY224 pKa = 9.89NSTISAEE231 pKa = 3.89QHH233 pKa = 5.68VNSGSEE239 pKa = 4.02FEE241 pKa = 4.85KK242 pKa = 11.14GNEE245 pKa = 4.27SSDD248 pKa = 3.31KK249 pKa = 10.67LYY251 pKa = 10.45PLVMPSFDD259 pKa = 3.98TDD261 pKa = 3.19PHH263 pKa = 6.55IWLPPDD269 pKa = 4.12PVNKK273 pKa = 10.43DD274 pKa = 3.22DD275 pKa = 5.16DD276 pKa = 4.13TDD278 pKa = 3.52IVANNDD284 pKa = 3.61DD285 pKa = 3.93NSDD288 pKa = 3.79NNGNWVQSSFNISFDD303 pKa = 3.09AKK305 pKa = 10.42RR306 pKa = 11.84NKK308 pKa = 9.02TSCEE312 pKa = 3.94DD313 pKa = 3.24QQ314 pKa = 3.61

Molecular weight: 34.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0E0J0V6|A0A0E0J0V6_ORYNI Ribonuclease P/MRP protein subunit POP5 OS=Oryza nivara OX=4536 PE=3 SV=1
MM1 pKa = 7.83PSLTTGRR8 pKa = 11.84SPWRR12 pKa = 11.84RR13 pKa = 11.84RR14 pKa = 11.84KK15 pKa = 10.25RR16 pKa = 11.84GTATTARR23 pKa = 11.84RR24 pKa = 11.84RR25 pKa = 11.84RR26 pKa = 11.84TPTSLRR32 pKa = 11.84ALWRR36 pKa = 11.84RR37 pKa = 11.84IVPRR41 pKa = 11.84TSTTTAPRR49 pKa = 11.84VRR51 pKa = 11.84TRR53 pKa = 11.84KK54 pKa = 9.6PGLLSRR60 pKa = 11.84ALRR63 pKa = 11.84VLSCGGGRR71 pKa = 11.84RR72 pKa = 11.84SRR74 pKa = 11.84AARR77 pKa = 11.84RR78 pKa = 11.84WW79 pKa = 3.29

Molecular weight: 9.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

36306

9870

46176

19239447

7

5485

416.7

45.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.148 ± 0.018

1.944 ± 0.005

5.372 ± 0.009

5.962 ± 0.013

3.62 ± 0.007

7.408 ± 0.014

2.516 ± 0.006

4.494 ± 0.009

4.833 ± 0.011

9.587 ± 0.017

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.432 ± 0.004

3.561 ± 0.01

5.545 ± 0.012

3.401 ± 0.008

6.419 ± 0.012

8.395 ± 0.011

4.809 ± 0.007

6.701 ± 0.009

1.319 ± 0.004

2.535 ± 0.006
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski