Verrucomicrobium sp. GAS474

Taxonomy: cellular organisms; Bacteria; PVC group; Verrucomicrobia; Verrucomicrobiae; Verrucomicrobiales; Verrucomicrobiaceae; Verrucomicrobium; unclassified Verrucomicrobium

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3180 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H2GZN7|A0A1H2GZN7_9BACT Uncharacterized protein OS=Verrucomicrobium sp. GAS474 OX=1882831 GN=SAMN05444156_2845 PE=4 SV=1
MM1 pKa = 7.5SISTVSSATASGSTTASTSSNASMSTADD29 pKa = 3.81FYY31 pKa = 11.64KK32 pKa = 10.44IIVAQLQNQSPDD44 pKa = 3.32DD45 pKa = 4.06TTDD48 pKa = 3.18SNAMVQNMMAISNYY62 pKa = 7.71QAINGMSTDD71 pKa = 3.47TTKK74 pKa = 11.05LSNYY78 pKa = 8.16STANSLLGKK87 pKa = 8.06TVTVTPSTLSSDD99 pKa = 3.82TVTGTVDD106 pKa = 2.95NVTYY110 pKa = 10.74SSDD113 pKa = 3.02GTASITINNNSYY125 pKa = 11.15DD126 pKa = 3.88LSTVLSVANTASSTSTGTTAQQ147 pKa = 3.33

Molecular weight: 14.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H2F2S1|A0A1H2F2S1_9BACT tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase OS=Verrucomicrobium sp. GAS474 OX=1882831 GN=miaB PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.69KK10 pKa = 9.77RR11 pKa = 11.84RR12 pKa = 11.84VRR14 pKa = 11.84QHH16 pKa = 5.75GFLARR21 pKa = 11.84TRR23 pKa = 11.84TKK25 pKa = 9.94SGRR28 pKa = 11.84AILSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 10.09GRR39 pKa = 11.84VRR41 pKa = 11.84LVPVGADD48 pKa = 3.03VKK50 pKa = 10.28FARR53 pKa = 11.84HH54 pKa = 4.39TQAA57 pKa = 4.79

Molecular weight: 6.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3180

0

3180

1135375

30

3253

357.0

38.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.517 ± 0.062

0.84 ± 0.013

4.858 ± 0.029

5.782 ± 0.053

4.065 ± 0.029

8.613 ± 0.057

2.077 ± 0.023

4.918 ± 0.032

4.06 ± 0.042

10.77 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.796 ± 0.017

2.904 ± 0.037

5.674 ± 0.047

3.001 ± 0.024

6.419 ± 0.053

6.02 ± 0.055

5.687 ± 0.064

6.999 ± 0.032

1.531 ± 0.023

2.467 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski