Pseudonocardia sediminis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Pseudonocardia

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6113 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q7URP2|A0A4Q7URP2_9PSEU Acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II OS=Pseudonocardia sediminis OX=1397368 GN=EV383_0243 PE=4 SV=1
MM1 pKa = 7.46LAILGVAAAIAIGFATTGTSPAPVATPVAPPAPSTSQLLAQWRR44 pKa = 11.84DD45 pKa = 3.43GGGLQHH51 pKa = 7.02LTTISGDD58 pKa = 3.44LTSVGEE64 pKa = 3.84AASRR68 pKa = 11.84YY69 pKa = 8.97DD70 pKa = 3.34VSGMMSACYY79 pKa = 10.09SLQNDD84 pKa = 3.8IEE86 pKa = 4.45SAQAFTPVPDD96 pKa = 3.81VQVQSSWSAALASGARR112 pKa = 11.84SAAYY116 pKa = 9.55CVAGAQQLDD125 pKa = 3.62PDD127 pKa = 5.51LINMSTTEE135 pKa = 4.1MNDD138 pKa = 3.34MTSHH142 pKa = 7.2LDD144 pKa = 3.48DD145 pKa = 3.38ATARR149 pKa = 11.84LNSINGII156 pKa = 3.9

Molecular weight: 15.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q7V4H5|A0A4Q7V4H5_9PSEU Phospholipid/cholesterol/gamma-HCH transport system ATP-binding protein OS=Pseudonocardia sediminis OX=1397368 GN=EV383_5350 PE=4 SV=1
MM1 pKa = 6.83TAALRR6 pKa = 11.84TGSGRR11 pKa = 11.84ATGRR15 pKa = 11.84RR16 pKa = 11.84WSLSRR21 pKa = 11.84PARR24 pKa = 11.84SALRR28 pKa = 11.84GGHH31 pKa = 5.06VVLGVARR38 pKa = 11.84TGMALVMLVTATLLSVRR55 pKa = 11.84KK56 pKa = 8.25PGRR59 pKa = 11.84RR60 pKa = 11.84VRR62 pKa = 11.84SLTGNRR68 pKa = 11.84KK69 pKa = 8.47VAA71 pKa = 3.51

Molecular weight: 7.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6113

0

6113

1958073

29

6843

320.3

34.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.696 ± 0.044

0.733 ± 0.009

6.599 ± 0.031

5.361 ± 0.025

2.605 ± 0.022

9.881 ± 0.029

2.18 ± 0.017

3.174 ± 0.022

1.524 ± 0.021

10.057 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.738 ± 0.011

1.587 ± 0.016

6.373 ± 0.032

2.423 ± 0.021

8.23 ± 0.034

5.193 ± 0.024

6.193 ± 0.023

9.241 ± 0.034

1.449 ± 0.013

1.763 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski