Klebsiella phage vB_KpnM_FZ14

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Jedunavirus; unclassified Jedunavirus

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 35 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6T3S7|A0A4D6T3S7_9CAUD DNA-directed DNA polymerase OS=Klebsiella phage vB_KpnM_FZ14 OX=2530028 GN=FZ14_14 PE=4 SV=1
MM1 pKa = 7.92AEE3 pKa = 4.19LTSTGYY9 pKa = 9.8SVKK12 pKa = 10.61SQNDD16 pKa = 3.0WFDD19 pKa = 3.59EE20 pKa = 4.25EE21 pKa = 4.28KK22 pKa = 10.67QLYY25 pKa = 10.35LDD27 pKa = 5.34IDD29 pKa = 4.75SNWNLDD35 pKa = 3.15PSTPDD40 pKa = 3.47GLKK43 pKa = 9.09MAHH46 pKa = 6.79DD47 pKa = 4.33AEE49 pKa = 4.65IFSALDD55 pKa = 3.24EE56 pKa = 4.48VLQQAYY62 pKa = 10.17NSKK65 pKa = 10.83DD66 pKa = 3.36PNKK69 pKa = 10.71ASGYY73 pKa = 10.2DD74 pKa = 3.51LDD76 pKa = 4.94VICALTGTVRR86 pKa = 11.84SEE88 pKa = 4.03GTASTVTGFVLTGVAGTQVPAGTRR112 pKa = 11.84FEE114 pKa = 4.52SSVTGYY120 pKa = 10.93RR121 pKa = 11.84FTLDD125 pKa = 3.18QTWTLDD131 pKa = 3.37SSGTATVDD139 pKa = 3.23ITCTTVGEE147 pKa = 4.34IEE149 pKa = 4.58ADD151 pKa = 3.23ANTITTIVDD160 pKa = 3.73TVAGLVSVNNPTPATPGTSAEE181 pKa = 4.23SDD183 pKa = 3.05GSLRR187 pKa = 11.84LKK189 pKa = 10.49RR190 pKa = 11.84ATAVGLPGSNQVDD203 pKa = 3.73SMLGQLFNVDD213 pKa = 2.55GVRR216 pKa = 11.84RR217 pKa = 11.84VRR219 pKa = 11.84VYY221 pKa = 11.09EE222 pKa = 4.02NDD224 pKa = 3.25EE225 pKa = 4.03ATTDD229 pKa = 3.55SNGQPGHH236 pKa = 6.53SIAPIVDD243 pKa = 3.4GGTDD247 pKa = 4.0DD248 pKa = 4.34DD249 pKa = 4.11VAMAIYY255 pKa = 10.12LKK257 pKa = 10.48KK258 pKa = 10.61NPGVALYY265 pKa = 10.23QAGTGVTVTVTSPTCSTMTKK285 pKa = 9.79DD286 pKa = 3.32IKK288 pKa = 10.47FSRR291 pKa = 11.84PVYY294 pKa = 9.2VDD296 pKa = 2.87MVVVIEE302 pKa = 4.51IKK304 pKa = 10.65DD305 pKa = 3.9DD306 pKa = 3.66GTLPSQATLEE316 pKa = 4.24PLIQDD321 pKa = 4.96AIMEE325 pKa = 4.3YY326 pKa = 10.66AAGGLIPTEE335 pKa = 4.23YY336 pKa = 10.73GFKK339 pKa = 10.12PDD341 pKa = 3.92GFDD344 pKa = 2.96IGEE347 pKa = 4.3TVPYY351 pKa = 10.09SSLYY355 pKa = 9.26TPINKK360 pKa = 9.51VIGSYY365 pKa = 10.9GNSYY369 pKa = 9.48VNSMTLNGGTANVTIKK385 pKa = 10.62FNEE388 pKa = 3.93LSRR391 pKa = 11.84WTTSNITVTIVV402 pKa = 2.6

Molecular weight: 42.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6T3W2|A0A4D6T3W2_9CAUD Uncharacterized protein OS=Klebsiella phage vB_KpnM_FZ14 OX=2530028 GN=FZ14_26 PE=4 SV=1
MM1 pKa = 7.51KK2 pKa = 10.11EE3 pKa = 3.53KK4 pKa = 10.79ALTQEE9 pKa = 4.05TLKK12 pKa = 11.24ALLHH16 pKa = 5.89YY17 pKa = 10.79DD18 pKa = 3.38PVTGVFTWLVSPSFTVKK35 pKa = 10.76AGDD38 pKa = 3.24KK39 pKa = 10.52AGNVTPKK46 pKa = 10.35GYY48 pKa = 8.73WQIGINRR55 pKa = 11.84KK56 pKa = 9.56RR57 pKa = 11.84YY58 pKa = 8.52LAHH61 pKa = 6.87RR62 pKa = 11.84LAWLYY67 pKa = 9.49VHH69 pKa = 6.86GVFPEE74 pKa = 3.87KK75 pKa = 10.74DD76 pKa = 3.02VDD78 pKa = 4.25HH79 pKa = 7.22INRR82 pKa = 11.84NPADD86 pKa = 3.55NRR88 pKa = 11.84IVNLRR93 pKa = 11.84DD94 pKa = 3.11VSNQFNLFNTSKK106 pKa = 10.86QKK108 pKa = 11.38DD109 pKa = 3.5NTSGYY114 pKa = 10.24KK115 pKa = 10.2GVTFIPKK122 pKa = 8.21VKK124 pKa = 9.74KK125 pKa = 8.3WQAQIRR131 pKa = 11.84MNSKK135 pKa = 9.97KK136 pKa = 10.74VYY138 pKa = 10.24LGTFDD143 pKa = 3.38TPEE146 pKa = 4.02RR147 pKa = 11.84ADD149 pKa = 3.18IAHH152 pKa = 7.49RR153 pKa = 11.84IAEE156 pKa = 4.27YY157 pKa = 10.41FRR159 pKa = 11.84EE160 pKa = 4.06KK161 pKa = 10.88YY162 pKa = 9.95YY163 pKa = 11.19DD164 pKa = 3.23HH165 pKa = 6.85STTKK169 pKa = 10.73GVV171 pKa = 3.09

Molecular weight: 19.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

35

0

35

10235

57

785

292.4

32.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.129 ± 0.405

1.436 ± 0.19

6.429 ± 0.224

5.53 ± 0.29

3.664 ± 0.224

7.386 ± 0.309

2.013 ± 0.239

5.716 ± 0.163

5.745 ± 0.35

7.416 ± 0.231

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.755 ± 0.155

4.944 ± 0.384

3.957 ± 0.189

4.358 ± 0.229

5.393 ± 0.273

6.273 ± 0.309

6.732 ± 0.427

7.035 ± 0.283

1.495 ± 0.138

3.596 ± 0.152
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski