Lettuce necrotic yellows virus (isolate 318) (LNYV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Betarhabdovirinae; Cytorhabdovirus; Lettuce necrotic yellows cytorhabdovirus; Lettuce necrotic yellows virus

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q9E7N8|MVP_LNYV3 Probable movement protein 4b OS=Lettuce necrotic yellows virus (isolate 318) OX=928304 GN=4b PE=2 SV=2
MM1 pKa = 8.03DD2 pKa = 4.95SEE4 pKa = 4.96SLDD7 pKa = 3.54FSSADD12 pKa = 3.57TVILRR17 pKa = 11.84SPNAGTNPDD26 pKa = 3.46GHH28 pKa = 7.43PDD30 pKa = 3.45TVEE33 pKa = 4.52CPDD36 pKa = 4.38FDD38 pKa = 4.08TDD40 pKa = 4.23IPKK43 pKa = 10.12TSDD46 pKa = 3.85DD47 pKa = 3.82SSKK50 pKa = 10.37MDD52 pKa = 3.48NKK54 pKa = 10.64GSSSSSKK61 pKa = 10.44AVKK64 pKa = 10.33DD65 pKa = 3.89LLEE68 pKa = 4.52LAAKK72 pKa = 9.74SQGIVVTDD80 pKa = 3.14VMQNTAIALHH90 pKa = 6.63HH91 pKa = 6.33NLGLDD96 pKa = 3.41ASSLDD101 pKa = 3.37WFVAGITFANNSMIMEE117 pKa = 4.37KK118 pKa = 9.48MVSAIKK124 pKa = 10.01EE125 pKa = 3.92LQIEE129 pKa = 4.18VRR131 pKa = 11.84NIQVASSGIKK141 pKa = 8.6GTSEE145 pKa = 3.81EE146 pKa = 4.78LVSKK150 pKa = 10.29MKK152 pKa = 10.72ANKK155 pKa = 9.61NDD157 pKa = 3.28IVKK160 pKa = 10.12EE161 pKa = 3.9LVKK164 pKa = 10.31TRR166 pKa = 11.84DD167 pKa = 3.57SVLSAMGGILSAPEE181 pKa = 4.26IEE183 pKa = 4.29QQPVKK188 pKa = 9.81TVTIGASQGRR198 pKa = 11.84RR199 pKa = 11.84KK200 pKa = 9.09STVVPPIEE208 pKa = 4.41INPEE212 pKa = 3.84LEE214 pKa = 4.19SPVLSKK220 pKa = 9.69TVSTATPEE228 pKa = 3.5EE229 pKa = 4.59RR230 pKa = 11.84IRR232 pKa = 11.84HH233 pKa = 4.98EE234 pKa = 4.18KK235 pKa = 10.4EE236 pKa = 3.59KK237 pKa = 11.09LLADD241 pKa = 4.74LDD243 pKa = 4.06WEE245 pKa = 4.25IGEE248 pKa = 4.24IAQYY252 pKa = 10.35TPLIVDD258 pKa = 4.25FLVPDD263 pKa = 5.83DD264 pKa = 4.4ILAMAADD271 pKa = 4.35GLTPEE276 pKa = 4.88LKK278 pKa = 10.5EE279 pKa = 4.29KK280 pKa = 10.02IQNEE284 pKa = 3.99IIEE287 pKa = 4.07NHH289 pKa = 5.78IALMALEE296 pKa = 5.18EE297 pKa = 4.29YY298 pKa = 10.97SSS300 pKa = 3.38

Molecular weight: 32.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q9E7N9|MATRX_LNYV3 Matrix protein OS=Lettuce necrotic yellows virus (isolate 318) OX=928304 GN=M PE=2 SV=1
MM1 pKa = 7.73LDD3 pKa = 3.32VNSVRR8 pKa = 11.84KK9 pKa = 9.19HH10 pKa = 4.16VLKK13 pKa = 10.18TGSLTSAVGTGTIYY27 pKa = 10.21QGTYY31 pKa = 9.13NRR33 pKa = 11.84YY34 pKa = 9.34AKK36 pKa = 10.37KK37 pKa = 10.31KK38 pKa = 8.14EE39 pKa = 3.71LNIIVTSSGSNNVIMRR55 pKa = 11.84QVPLFDD61 pKa = 5.88KK62 pKa = 10.89EE63 pKa = 5.17DD64 pKa = 3.96LDD66 pKa = 4.72AMKK69 pKa = 10.79SDD71 pKa = 3.69TTSNKK76 pKa = 9.37YY77 pKa = 10.06LHH79 pKa = 6.89IGCITVSIEE88 pKa = 3.81PLLHH92 pKa = 4.94QRR94 pKa = 11.84YY95 pKa = 7.25MKK97 pKa = 10.92NFGKK101 pKa = 9.12TIAGNCAIIDD111 pKa = 3.75STFRR115 pKa = 11.84KK116 pKa = 9.46VDD118 pKa = 3.18QSIISLHH125 pKa = 6.57KK126 pKa = 9.56YY127 pKa = 9.56DD128 pKa = 4.72LSRR131 pKa = 11.84GRR133 pKa = 11.84ADD135 pKa = 3.28YY136 pKa = 11.03VSYY139 pKa = 10.7PNHH142 pKa = 6.6CLSLTDD148 pKa = 3.86PMIQKK153 pKa = 10.16RR154 pKa = 11.84LSVLLGIKK162 pKa = 10.36GIDD165 pKa = 3.48VEE167 pKa = 4.59PGVEE171 pKa = 4.36LFSICIGYY179 pKa = 9.33IVSSVNTLHH188 pKa = 6.97PVSQLGIQGVAINGTEE204 pKa = 3.98SADD207 pKa = 3.38IDD209 pKa = 3.77EE210 pKa = 5.56LGAEE214 pKa = 5.33DD215 pKa = 5.11IDD217 pKa = 4.53QLSLSYY223 pKa = 10.82NDD225 pKa = 3.99SKK227 pKa = 11.08IISLPSDD234 pKa = 2.96EE235 pKa = 5.47DD236 pKa = 3.0IYY238 pKa = 11.47YY239 pKa = 10.34RR240 pKa = 11.84SKK242 pKa = 11.35GSLFSKK248 pKa = 10.43GRR250 pKa = 11.84TIKK253 pKa = 10.32RR254 pKa = 11.84RR255 pKa = 11.84TMRR258 pKa = 11.84TRR260 pKa = 11.84VPDD263 pKa = 3.7PEE265 pKa = 4.27EE266 pKa = 4.59PIKK269 pKa = 10.09LTKK272 pKa = 10.14SQSSRR277 pKa = 11.84IEE279 pKa = 3.7HH280 pKa = 5.65GKK282 pKa = 10.06VMRR285 pKa = 11.84LLKK288 pKa = 10.51NKK290 pKa = 9.59QIRR293 pKa = 11.84EE294 pKa = 4.03KK295 pKa = 10.46IEE297 pKa = 3.92RR298 pKa = 11.84GMIAA302 pKa = 3.92

Molecular weight: 33.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

3857

177

2068

642.8

72.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.6 ± 1.604

1.815 ± 0.362

6.222 ± 0.363

5.963 ± 0.375

3.189 ± 0.447

5.626 ± 0.309

2.126 ± 0.211

6.611 ± 0.664

6.404 ± 0.264

9.437 ± 0.493

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.267 ± 0.393

3.837 ± 0.134

4.511 ± 0.142

2.904 ± 0.434

5.548 ± 0.477

8.608 ± 0.616

6.663 ± 0.52

6.482 ± 0.303

1.685 ± 0.338

3.5 ± 0.426
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski