McMurdo Ice Shelf pond-associated circular DNA virus-8

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 7.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A075LZ83|A0A075LZ83_9VIRU Uncharacterized protein OS=McMurdo Ice Shelf pond-associated circular DNA virus-8 OX=1521392 PE=4 SV=1
MM1 pKa = 7.5CGRR4 pKa = 11.84WGFRR8 pKa = 11.84DD9 pKa = 3.46WDD11 pKa = 3.71PVEE14 pKa = 4.03ARR16 pKa = 11.84LYY18 pKa = 10.3QPTVQKK24 pKa = 10.97VLEE27 pKa = 5.01DD28 pKa = 4.4YY29 pKa = 11.35GDD31 pKa = 3.47DD32 pKa = 3.82WIFQLEE38 pKa = 4.15MGEE41 pKa = 4.39SGFVHH46 pKa = 5.66YY47 pKa = 10.22QIYY50 pKa = 9.13WKK52 pKa = 8.98LTKK55 pKa = 9.51RR56 pKa = 11.84DD57 pKa = 3.67RR58 pKa = 11.84PKK60 pKa = 10.39TLAIALNEE68 pKa = 3.92RR69 pKa = 11.84LPGIEE74 pKa = 4.88LSAASTGGVLALKK87 pKa = 9.61RR88 pKa = 11.84YY89 pKa = 9.12CMKK92 pKa = 10.84DD93 pKa = 3.25DD94 pKa = 3.58TRR96 pKa = 11.84LAGPWSKK103 pKa = 11.07KK104 pKa = 9.59KK105 pKa = 10.15IYY107 pKa = 10.08RR108 pKa = 11.84GEE110 pKa = 4.17DD111 pKa = 3.48LPVKK115 pKa = 9.45MLPWQQKK122 pKa = 9.16LADD125 pKa = 3.97YY126 pKa = 10.81LKK128 pKa = 9.17TEE130 pKa = 4.0AGKK133 pKa = 10.7RR134 pKa = 11.84EE135 pKa = 4.71IIWLMDD141 pKa = 4.06PLGKK145 pKa = 9.23MGKK148 pKa = 8.12TIFGKK153 pKa = 9.99MMAYY157 pKa = 8.86WRR159 pKa = 11.84GALYY163 pKa = 8.06LTWGDD168 pKa = 3.68TKK170 pKa = 11.54DD171 pKa = 4.11VMNLVAKK178 pKa = 10.16SDD180 pKa = 3.73KK181 pKa = 10.69NNVYY185 pKa = 10.1IFNLSRR191 pKa = 11.84TKK193 pKa = 10.48PKK195 pKa = 10.25MMSSDD200 pKa = 3.91DD201 pKa = 4.48LYY203 pKa = 11.17TALEE207 pKa = 4.1QIKK210 pKa = 10.65DD211 pKa = 3.46GAFINTKK218 pKa = 10.38YY219 pKa = 8.27EE220 pKa = 4.03TSQVLMKK227 pKa = 9.75PPHH230 pKa = 5.65VVVMANCMPEE240 pKa = 3.85MAKK243 pKa = 10.39LSKK246 pKa = 10.66DD247 pKa = 2.88RR248 pKa = 11.84WNVIVMKK255 pKa = 10.62FSDD258 pKa = 3.94RR259 pKa = 11.84PPVEE263 pKa = 3.75EE264 pKa = 3.77EE265 pKa = 3.96TRR267 pKa = 11.84FTMEE271 pKa = 4.4DD272 pKa = 3.27PVKK275 pKa = 9.96EE276 pKa = 4.06RR277 pKa = 11.84EE278 pKa = 4.38GGGSSSQEE286 pKa = 3.87TVVLSQEE293 pKa = 3.97EE294 pKa = 4.61HH295 pKa = 6.23EE296 pKa = 4.63VMGDD300 pKa = 3.39QFPDD304 pKa = 3.25SSAPTGASVV313 pKa = 3.48

Molecular weight: 35.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A075LZ83|A0A075LZ83_9VIRU Uncharacterized protein OS=McMurdo Ice Shelf pond-associated circular DNA virus-8 OX=1521392 PE=4 SV=1
MM1 pKa = 7.23GRR3 pKa = 11.84DD4 pKa = 3.51VTPTSWTTCLEE15 pKa = 3.87GEE17 pKa = 4.65MYY19 pKa = 10.73GSHH22 pKa = 5.82TRR24 pKa = 11.84NHH26 pKa = 7.16DD27 pKa = 3.64SQLLDD32 pKa = 3.15SSEE35 pKa = 4.04IVSGEE40 pKa = 3.78RR41 pKa = 11.84IDD43 pKa = 5.33RR44 pKa = 11.84VGSDD48 pKa = 3.38LRR50 pKa = 11.84LARR53 pKa = 11.84ALEE56 pKa = 4.33GVGLARR62 pKa = 11.84KK63 pKa = 9.78SSALSLLGNSGTLGLHH79 pKa = 6.32GDD81 pKa = 4.11DD82 pKa = 3.39VGVLNGVPRR91 pKa = 11.84NLNRR95 pKa = 11.84EE96 pKa = 4.08LQTDD100 pKa = 4.35GEE102 pKa = 4.49LGLNGGGAGMTPPDD116 pKa = 3.64PRR118 pKa = 11.84AVEE121 pKa = 4.11RR122 pKa = 11.84VLSGRR127 pKa = 11.84RR128 pKa = 11.84HH129 pKa = 5.8HH130 pKa = 6.78NGGVDD135 pKa = 3.23RR136 pKa = 11.84RR137 pKa = 11.84KK138 pKa = 8.38TRR140 pKa = 11.84VLDD143 pKa = 3.45EE144 pKa = 4.19VLAVLIVDD152 pKa = 3.14QHH154 pKa = 7.28SGVRR158 pKa = 11.84FTADD162 pKa = 3.35DD163 pKa = 4.53TITLANRR170 pKa = 11.84HH171 pKa = 5.76NSLVSPTLDD180 pKa = 3.08VRR182 pKa = 11.84EE183 pKa = 3.87VDD185 pKa = 3.52GRR187 pKa = 11.84EE188 pKa = 3.9TRR190 pKa = 11.84SGSIRR195 pKa = 11.84VRR197 pKa = 11.84VAGMRR202 pKa = 11.84AAEE205 pKa = 4.3DD206 pKa = 3.21QTTVVRR212 pKa = 11.84LRR214 pKa = 11.84GDD216 pKa = 3.3SSIVRR221 pKa = 11.84VHH223 pKa = 6.38HH224 pKa = 5.87NRR226 pKa = 11.84VGEE229 pKa = 4.08RR230 pKa = 11.84NHH232 pKa = 6.24AHH234 pKa = 6.97LKK236 pKa = 9.91RR237 pKa = 11.84VNSHH241 pKa = 5.85PLEE244 pKa = 4.32AVVEE248 pKa = 4.19VLALFRR254 pKa = 11.84EE255 pKa = 4.61VEE257 pKa = 3.97EE258 pKa = 5.04GGAEE262 pKa = 3.99RR263 pKa = 11.84TDD265 pKa = 3.59TGQGTAQEE273 pKa = 4.24QLEE276 pKa = 4.51SLSTTSSGVGHH287 pKa = 6.89GSSEE291 pKa = 3.7EE292 pKa = 3.73RR293 pKa = 11.84RR294 pKa = 11.84RR295 pKa = 11.84RR296 pKa = 11.84SAQGGRR302 pKa = 11.84VGVVHH307 pKa = 6.79LVGAGDD313 pKa = 4.29LATVQPGVRR322 pKa = 11.84HH323 pKa = 6.57RR324 pKa = 11.84DD325 pKa = 3.33RR326 pKa = 11.84PHH328 pKa = 6.37EE329 pKa = 4.22VGGTPLRR336 pKa = 11.84GTLTPTSLSTAVLTGRR352 pKa = 11.84DD353 pKa = 3.96GACRR357 pKa = 11.84ALACPFLWNDD367 pKa = 3.27LEE369 pKa = 4.34IVVVGQIGAFHH380 pKa = 7.01RR381 pKa = 11.84RR382 pKa = 11.84QGRR385 pKa = 11.84QGCLRR390 pKa = 11.84ALLRR394 pKa = 11.84VLEE397 pKa = 4.33LVVPRR402 pKa = 11.84DD403 pKa = 3.34GSSWRR408 pKa = 11.84RR409 pKa = 11.84TDD411 pKa = 3.61RR412 pKa = 11.84RR413 pKa = 11.84MGTLKK418 pKa = 10.44RR419 pKa = 11.84RR420 pKa = 11.84LCEE423 pKa = 4.35GGNGPHH429 pKa = 5.6TTSTRR434 pKa = 11.84IWFMDD439 pKa = 3.8LFQNTSDD446 pKa = 3.35

Molecular weight: 48.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1167

313

446

389.0

42.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.141 ± 1.806

1.285 ± 0.178

5.741 ± 0.567

5.484 ± 0.754

2.656 ± 0.651

8.997 ± 1.525

2.485 ± 0.721

3.342 ± 0.625

4.199 ± 1.732

8.483 ± 1.164

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.742 ± 0.966

3.428 ± 0.334

4.97 ± 0.889

3.599 ± 0.375

7.883 ± 2.136

7.198 ± 0.481

7.369 ± 0.647

8.226 ± 0.85

1.628 ± 0.503

2.142 ± 0.88
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski