Thermosipho atlanticus DSM 15807

Taxonomy: cellular organisms; Bacteria; Thermotogae; Thermotogae; Thermotogales; Fervidobacteriaceae; Thermosipho; Thermosipho atlanticus

Average proteome isoelectric point is 7.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1560 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M5T4M1|A0A1M5T4M1_9BACT Fe-ADH domain-containing protein OS=Thermosipho atlanticus DSM 15807 OX=1123380 GN=SAMN02745199_1167 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 10.45RR3 pKa = 11.84LFLILLFLLVLVFVLWSCGLQLPNSVTVSYY33 pKa = 11.03SNHH36 pKa = 5.84FEE38 pKa = 4.01FPLAMLHH45 pKa = 5.69FTLDD49 pKa = 3.57DD50 pKa = 4.76FINPVLLSLEE60 pKa = 4.13NEE62 pKa = 4.44GFQVTTGDD70 pKa = 4.46PITISFATTTTFIPGDD86 pKa = 3.95YY87 pKa = 9.77LPTGIPISGTEE98 pKa = 4.18TILDD102 pKa = 3.69QATLIQASTMQNGNVLQNVDD122 pKa = 4.14FNMSFEE128 pKa = 4.25VGYY131 pKa = 9.45FASTTTFDD139 pKa = 3.56STLVFYY145 pKa = 10.73INSTPVVISEE155 pKa = 4.2NSTEE159 pKa = 4.25SEE161 pKa = 4.06NLTKK165 pKa = 10.57YY166 pKa = 10.15VKK168 pKa = 10.19EE169 pKa = 4.08VLKK172 pKa = 10.83SGQDD176 pKa = 3.2LTVRR180 pKa = 11.84ADD182 pKa = 2.99IDD184 pKa = 3.37IDD186 pKa = 3.88GTIQSSDD193 pKa = 3.29EE194 pKa = 4.08LMLGVNWTFSLEE206 pKa = 4.1GTTLADD212 pKa = 3.06IVFDD216 pKa = 5.05ASTTDD221 pKa = 3.6LSVLEE226 pKa = 4.46SLTDD230 pKa = 4.0FVDD233 pKa = 3.24SATIVFDD240 pKa = 3.2EE241 pKa = 4.62WDD243 pKa = 3.44NSLGFDD249 pKa = 3.53TVFDD253 pKa = 3.79VGNLSFYY260 pKa = 10.88FGTTPPIVGLSKK272 pKa = 11.09DD273 pKa = 3.91DD274 pKa = 5.06LISIATDD281 pKa = 3.06NVPYY285 pKa = 10.16VIKK288 pKa = 10.9VPANSYY294 pKa = 10.64IKK296 pKa = 10.56LKK298 pKa = 10.7SNSYY302 pKa = 10.4LDD304 pKa = 3.5SAVYY308 pKa = 10.04ISLDD312 pKa = 3.34LTVATEE318 pKa = 4.09VTFF321 pKa = 4.26

Molecular weight: 35.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M5RC36|A0A1M5RC36_9BACT Adenine-specific DNA-methyltransferase OS=Thermosipho atlanticus DSM 15807 OX=1123380 GN=SAMN02745199_0462 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 9.91QPSRR9 pKa = 11.84IKK11 pKa = 10.52RR12 pKa = 11.84KK13 pKa = 8.24RR14 pKa = 11.84THH16 pKa = 6.29GFLARR21 pKa = 11.84KK22 pKa = 7.96RR23 pKa = 11.84TASGRR28 pKa = 11.84KK29 pKa = 7.3VLKK32 pKa = 9.48NRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.77GRR39 pKa = 11.84WRR41 pKa = 11.84LAVV44 pKa = 3.35

Molecular weight: 5.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1560

0

1560

501096

38

1650

321.2

36.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.23 ± 0.058

0.6 ± 0.017

4.784 ± 0.044

7.625 ± 0.072

5.683 ± 0.071

6.405 ± 0.064

1.448 ± 0.024

9.956 ± 0.056

9.396 ± 0.064

9.688 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.152 ± 0.024

5.395 ± 0.051

3.442 ± 0.033

2.019 ± 0.022

3.632 ± 0.04

5.725 ± 0.048

4.673 ± 0.038

6.951 ± 0.056

0.877 ± 0.021

4.318 ± 0.045
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski