Apis mellifera associated microvirus 44

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A3Q8U598|A0A3Q8U598_9VIRU Replication initiator protein OS=Apis mellifera associated microvirus 44 OX=2494774 PE=4 SV=1
MM1 pKa = 7.44SSKK4 pKa = 10.51KK5 pKa = 9.89PSTEE9 pKa = 3.5IPFDD13 pKa = 3.36IAPRR17 pKa = 11.84APRR20 pKa = 11.84FQNLNTLGYY29 pKa = 9.95SIADD33 pKa = 3.97PTPVAPPIGYY43 pKa = 8.76QKK45 pKa = 9.64PISMVDD51 pKa = 2.88QMRR54 pKa = 11.84NLIRR58 pKa = 11.84SEE60 pKa = 3.76MLARR64 pKa = 11.84AVAAQGMEE72 pKa = 4.1TFEE75 pKa = 4.17EE76 pKa = 4.67SEE78 pKa = 4.58DD79 pKa = 3.96FDD81 pKa = 5.16VPDD84 pKa = 5.47DD85 pKa = 5.07DD86 pKa = 5.95GVLDD90 pKa = 4.1PHH92 pKa = 7.09SPWEE96 pKa = 4.03NDD98 pKa = 2.61FDD100 pKa = 4.31PPARR104 pKa = 11.84EE105 pKa = 3.54IAEE108 pKa = 4.14AVGDD112 pKa = 3.92ARR114 pKa = 11.84QRR116 pKa = 11.84PPMPAEE122 pKa = 4.38GGGGGAAPAEE132 pKa = 4.28QPKK135 pKa = 9.45PASPAPAPEE144 pKa = 4.58APPAKK149 pKa = 9.7PALPP153 pKa = 4.09

Molecular weight:
16.25 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A3S8UTI2|A0A3S8UTI2_9VIRU Uncharacterized protein OS=Apis mellifera associated microvirus 44 OX=2494774 PE=4 SV=1
MM1 pKa = 7.65KK2 pKa = 10.6GGGLNGLSSSSSSDD16 pKa = 3.16CSSSSRR22 pKa = 11.84SRR24 pKa = 11.84SSSSRR29 pKa = 11.84SRR31 pKa = 11.84APNRR35 pKa = 11.84VSHH38 pKa = 5.79VRR40 pKa = 11.84CEE42 pKa = 4.1NPFVRR47 pKa = 11.84GNSVHH52 pKa = 6.25GCGRR56 pKa = 11.84CLPCRR61 pKa = 11.84INRR64 pKa = 11.84RR65 pKa = 11.84RR66 pKa = 11.84LWKK69 pKa = 10.4VRR71 pKa = 11.84LGLEE75 pKa = 4.24ALLHH79 pKa = 5.36SQSAFVTLTYY89 pKa = 10.72ADD91 pKa = 3.89EE92 pKa = 4.42NLPVAKK98 pKa = 10.11SSKK101 pKa = 10.61GSLATLDD108 pKa = 4.0PVDD111 pKa = 3.94LRR113 pKa = 11.84NWLKK117 pKa = 10.64RR118 pKa = 11.84LRR120 pKa = 11.84KK121 pKa = 9.6AISPSSIRR129 pKa = 11.84YY130 pKa = 7.64YY131 pKa = 11.24AVGEE135 pKa = 4.2YY136 pKa = 10.63GDD138 pKa = 4.16EE139 pKa = 4.0SWRR142 pKa = 11.84PHH144 pKa = 4.14YY145 pKa = 10.57HH146 pKa = 6.46LALFGYY152 pKa = 7.51PRR154 pKa = 11.84CSTLQTTYY162 pKa = 11.01RR163 pKa = 11.84LRR165 pKa = 11.84ASTSEE170 pKa = 3.97KK171 pKa = 10.34PKK173 pKa = 10.95CCDD176 pKa = 2.82HH177 pKa = 6.88CRR179 pKa = 11.84LIHH182 pKa = 6.93DD183 pKa = 3.53SWGLGRR189 pKa = 11.84VQVAEE194 pKa = 4.75LNDD197 pKa = 3.43ASAGYY202 pKa = 9.38IAGYY206 pKa = 9.4IEE208 pKa = 4.63KK209 pKa = 10.41KK210 pKa = 9.06LTRR213 pKa = 11.84TDD215 pKa = 3.55DD216 pKa = 3.37PRR218 pKa = 11.84LDD220 pKa = 3.79GRR222 pKa = 11.84WPEE225 pKa = 4.01FARR228 pKa = 11.84MSLRR232 pKa = 11.84PGIGAGFMPHH242 pKa = 6.44YY243 pKa = 9.92FQALLEE249 pKa = 4.58HH250 pKa = 6.64NLDD253 pKa = 3.63GMADD257 pKa = 3.55VPSAIRR263 pKa = 11.84IGDD266 pKa = 3.57KK267 pKa = 10.41VVPLGRR273 pKa = 11.84YY274 pKa = 7.46LTSQLRR280 pKa = 11.84KK281 pKa = 10.31LNGKK285 pKa = 9.12DD286 pKa = 3.12GKK288 pKa = 10.78APPEE292 pKa = 3.7TLKK295 pKa = 11.09AVFEE299 pKa = 4.44EE300 pKa = 4.69KK301 pKa = 10.69VLPLLLAARR310 pKa = 11.84TSADD314 pKa = 3.31APSLKK319 pKa = 10.49EE320 pKa = 4.05QVLQKK325 pKa = 10.86NRR327 pKa = 11.84GARR330 pKa = 11.84SRR332 pKa = 11.84LLSLNKK338 pKa = 9.25IHH340 pKa = 7.12RR341 pKa = 11.84QRR343 pKa = 11.84KK344 pKa = 7.8HH345 pKa = 4.65LL346 pKa = 4.02

Molecular weight:
38.59 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1420

75

499

284.0

31.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.0 ± 1.423

0.845 ± 0.551

5.07 ± 0.575

4.507 ± 0.509

3.732 ± 0.68

8.099 ± 0.717

2.394 ± 0.334

4.085 ± 0.742

4.085 ± 0.737

7.746 ± 1.479

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.746 ± 0.613

4.366 ± 0.569

7.324 ± 1.679

4.296 ± 0.872

7.746 ± 1.367

8.028 ± 1.071

4.93 ± 1.017

5.211 ± 0.462

1.338 ± 0.278

3.451 ± 0.513

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski