Photobacterium profundum (strain SS9)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae; Photobacterium; Photobacterium profundum

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5328 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q6LTP6|Q6LTP6_PHOPR Putative polar flagellar protein FlaK OS=Photobacterium profundum (strain SS9) OX=298386 GN=PSPTO195 PE=4 SV=1
MM1 pKa = 7.15LTASLVGCADD11 pKa = 3.46TPEE14 pKa = 4.19NQEE17 pKa = 4.23KK18 pKa = 8.53EE19 pKa = 4.59TVPTNVAEE27 pKa = 4.24QINAEE32 pKa = 4.13EE33 pKa = 4.3FEE35 pKa = 4.71SVNDD39 pKa = 3.55VYY41 pKa = 11.68DD42 pKa = 3.89PLEE45 pKa = 4.16GFNRR49 pKa = 11.84VMWDD53 pKa = 3.28LNYY56 pKa = 10.98DD57 pKa = 3.65YY58 pKa = 10.95LDD60 pKa = 4.58PYY62 pKa = 10.23LARR65 pKa = 11.84PISLAYY71 pKa = 10.11VDD73 pKa = 4.12YY74 pKa = 10.21TPNPVRR80 pKa = 11.84LGIANFFANLDD91 pKa = 3.81EE92 pKa = 4.67PASMVNSLIMQNPEE106 pKa = 3.39DD107 pKa = 4.02AVAHH111 pKa = 6.5FNRR114 pKa = 11.84FWINTVFGIAGLIDD128 pKa = 3.29IASAADD134 pKa = 3.62IPPPSNRR141 pKa = 11.84AFGDD145 pKa = 3.38SLGYY149 pKa = 10.42YY150 pKa = 10.16GVGNGPYY157 pKa = 10.43FMVPFYY163 pKa = 11.43GPVTLRR169 pKa = 11.84EE170 pKa = 4.32GVGDD174 pKa = 3.95AADD177 pKa = 3.91GLYY180 pKa = 10.87LPLSLLTFWQSLGKK194 pKa = 9.01WAFEE198 pKa = 3.91GMEE201 pKa = 4.21DD202 pKa = 3.66RR203 pKa = 11.84AALVKK208 pKa = 10.53QEE210 pKa = 4.68ALLEE214 pKa = 4.53DD215 pKa = 4.01SPDD218 pKa = 3.48PYY220 pKa = 11.5VFTRR224 pKa = 11.84DD225 pKa = 4.02AYY227 pKa = 9.35IQHH230 pKa = 6.53KK231 pKa = 9.07NFRR234 pKa = 11.84ATGGEE239 pKa = 4.21TPVDD243 pKa = 3.54EE244 pKa = 5.2PEE246 pKa = 4.56NEE248 pKa = 4.15EE249 pKa = 4.59QLDD252 pKa = 4.14DD253 pKa = 4.11YY254 pKa = 11.49LDD256 pKa = 4.79EE257 pKa = 6.45IDD259 pKa = 5.85DD260 pKa = 4.03YY261 pKa = 12.23

Molecular weight: 29.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q6LHR5|Q6LHR5_PHOPR Uncharacterized protein OS=Photobacterium profundum (strain SS9) OX=298386 GN=PBPRB1294 PE=4 SV=1
MM1 pKa = 7.67GNQSIKK7 pKa = 9.78TLNASNTHH15 pKa = 6.7PIVLIRR21 pKa = 11.84GLLRR25 pKa = 11.84EE26 pKa = 3.93QRR28 pKa = 11.84HH29 pKa = 5.36WGDD32 pKa = 3.97FLNQLITQFPKK43 pKa = 10.59RR44 pKa = 11.84QIICIDD50 pKa = 3.32LAGNGKK56 pKa = 7.8RR57 pKa = 11.84HH58 pKa = 6.35KK59 pKa = 10.4LQSPKK64 pKa = 10.48NIPAMVQDD72 pKa = 6.05LRR74 pKa = 11.84IQLCLKK80 pKa = 9.17LTTDD84 pKa = 3.74TPVDD88 pKa = 5.0LIALSMGGMIAISWMTLFPTEE109 pKa = 3.79IRR111 pKa = 11.84SAILMNTSVRR121 pKa = 11.84PLSPFYY127 pKa = 10.82QRR129 pKa = 11.84LNWRR133 pKa = 11.84HH134 pKa = 4.16TT135 pKa = 3.48

Molecular weight: 15.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5328

0

5328

1694257

35

4684

318.0

35.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.403 ± 0.037

1.142 ± 0.012

5.529 ± 0.033

5.956 ± 0.03

4.104 ± 0.023

6.691 ± 0.032

2.263 ± 0.017

6.731 ± 0.027

5.405 ± 0.028

10.277 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.762 ± 0.016

4.5 ± 0.025

3.835 ± 0.02

4.447 ± 0.03

4.5 ± 0.029

6.655 ± 0.031

5.634 ± 0.031

6.832 ± 0.031

1.267 ± 0.013

3.067 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski