Hubei tombus-like virus 18

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KG77|A0A1L3KG77_9VIRU RNA-directed RNA polymerase OS=Hubei tombus-like virus 18 OX=1923264 PE=4 SV=1
MM1 pKa = 7.71ANAQPAQGIVGLEE14 pKa = 3.84NIIAQLQQLTQDD26 pKa = 4.06PNFDD30 pKa = 3.65PQLAEE35 pKa = 4.35DD36 pKa = 5.59LILATRR42 pKa = 11.84PVLEE46 pKa = 3.75AHH48 pKa = 6.66RR49 pKa = 11.84LRR51 pKa = 11.84RR52 pKa = 11.84EE53 pKa = 3.95RR54 pKa = 11.84VVLARR59 pKa = 11.84DD60 pKa = 3.55LLIALQDD67 pKa = 4.09GEE69 pKa = 5.29DD70 pKa = 3.72PAGDD74 pKa = 3.62GLGIPLVADD83 pKa = 3.49ALEE86 pKa = 4.27NVQRR90 pKa = 11.84EE91 pKa = 4.4IIRR94 pKa = 11.84EE95 pKa = 3.99RR96 pKa = 11.84GHH98 pKa = 6.6PGVPGGGMHH107 pKa = 7.26RR108 pKa = 11.84DD109 pKa = 3.0AGLFVGEE116 pKa = 4.2GNGARR121 pKa = 11.84EE122 pKa = 4.06RR123 pKa = 11.84APNDD127 pKa = 2.98AAAEE131 pKa = 3.99LRR133 pKa = 11.84DD134 pKa = 3.48LRR136 pKa = 11.84VVRR139 pKa = 11.84EE140 pKa = 3.96RR141 pKa = 11.84DD142 pKa = 3.02EE143 pKa = 4.34HH144 pKa = 6.11RR145 pKa = 11.84RR146 pKa = 11.84QLAEE150 pKa = 4.09LPDD153 pKa = 3.69NEE155 pKa = 5.24EE156 pKa = 3.73IFGDD160 pKa = 3.96VWWMFGDD167 pKa = 4.05EE168 pKa = 4.47EE169 pKa = 4.29PAAEE173 pKa = 4.26PPAGRR178 pKa = 11.84EE179 pKa = 3.76IAVGPDD185 pKa = 2.98IPGTAVPAKK194 pKa = 10.59VRR196 pKa = 11.84DD197 pKa = 3.8KK198 pKa = 11.18YY199 pKa = 10.98RR200 pKa = 11.84SNKK203 pKa = 8.43TDD205 pKa = 2.76IRR207 pKa = 11.84DD208 pKa = 3.98PLPEE212 pKa = 3.93ATRR215 pKa = 11.84DD216 pKa = 3.69LPEE219 pKa = 4.01FRR221 pKa = 11.84ADD223 pKa = 3.51LVSVQAGTVPLFTHH237 pKa = 6.79RR238 pKa = 11.84VIDD241 pKa = 4.09HH242 pKa = 7.07RR243 pKa = 11.84PLCDD247 pKa = 3.17QDD249 pKa = 4.99VYY251 pKa = 12.0AHH253 pKa = 6.38LQAVTMFMPRR263 pKa = 11.84TPGLAVYY270 pKa = 9.14MRR272 pKa = 11.84EE273 pKa = 3.58RR274 pKa = 11.84ALRR277 pKa = 11.84FVEE280 pKa = 5.73GYY282 pKa = 8.04DD283 pKa = 3.2TGRR286 pKa = 11.84LSRR289 pKa = 11.84MEE291 pKa = 3.81INEE294 pKa = 4.27IIGAAIANAYY304 pKa = 8.49VPSVAEE310 pKa = 3.8MQLIRR315 pKa = 11.84TAEE318 pKa = 4.28YY319 pKa = 10.1IPTQYY324 pKa = 11.23RR325 pKa = 11.84LDD327 pKa = 3.62RR328 pKa = 11.84FNEE331 pKa = 4.23FFRR334 pKa = 11.84TGDD337 pKa = 3.55SSSSAATGPKK347 pKa = 10.1NSLNKK352 pKa = 9.61VNVVRR357 pKa = 11.84KK358 pKa = 9.28CLSKK362 pKa = 10.54RR363 pKa = 11.84WIVGIGLLAMASLVILRR380 pKa = 11.84KK381 pKa = 8.9PQLPLHH387 pKa = 6.55IPTLSRR393 pKa = 11.84IMM395 pKa = 4.08

Molecular weight: 43.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KG35|A0A1L3KG35_9VIRU Uncharacterized protein OS=Hubei tombus-like virus 18 OX=1923264 PE=4 SV=1
MM1 pKa = 7.13QVNRR5 pKa = 11.84YY6 pKa = 8.8LGRR9 pKa = 11.84GRR11 pKa = 11.84GWRR14 pKa = 11.84DD15 pKa = 2.7WVVPGLQVGGYY26 pKa = 8.5LANSAIRR33 pKa = 11.84SYY35 pKa = 11.02LGRR38 pKa = 11.84PQTIVYY44 pKa = 7.36QQPDD48 pKa = 3.73PLASQMANMSIQPAQQSRR66 pKa = 11.84ARR68 pKa = 11.84GGRR71 pKa = 11.84RR72 pKa = 11.84RR73 pKa = 11.84GRR75 pKa = 11.84GGRR78 pKa = 11.84GRR80 pKa = 11.84GGRR83 pKa = 11.84GRR85 pKa = 11.84GRR87 pKa = 11.84GALPLIAPPGSGAIVAEE104 pKa = 4.17DD105 pKa = 3.74TEE107 pKa = 4.76IIVVKK112 pKa = 9.31TADD115 pKa = 3.48KK116 pKa = 9.44PAVVALVFNPAVTEE130 pKa = 4.22TPRR133 pKa = 11.84LLRR136 pKa = 11.84YY137 pKa = 9.81EE138 pKa = 4.31EE139 pKa = 3.84MFEE142 pKa = 4.24RR143 pKa = 11.84YY144 pKa = 8.63RR145 pKa = 11.84VVYY148 pKa = 9.27FRR150 pKa = 11.84INYY153 pKa = 9.43KK154 pKa = 9.85GACSANQRR162 pKa = 11.84GNIQFAITPEE172 pKa = 4.03AKK174 pKa = 9.93SANVTVDD181 pKa = 4.71KK182 pKa = 10.74IAQLKK187 pKa = 8.98PYY189 pKa = 10.09RR190 pKa = 11.84SVACWQTGSIKK201 pKa = 9.83VDD203 pKa = 3.13QRR205 pKa = 11.84VGEE208 pKa = 4.18TRR210 pKa = 11.84YY211 pKa = 10.26LEE213 pKa = 4.61CGGSQPAFTFYY224 pKa = 10.93YY225 pKa = 9.4VASGEE230 pKa = 4.15EE231 pKa = 4.37GQVVGNFHH239 pKa = 6.53VSYY242 pKa = 9.85KK243 pKa = 10.4IQFAYY248 pKa = 9.83PKK250 pKa = 10.58VFTT253 pKa = 4.69

Molecular weight: 27.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1279

241

395

319.8

36.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.74 ± 1.252

2.033 ± 0.652

4.222 ± 1.103

6.099 ± 0.65

3.362 ± 0.642

7.506 ± 1.173

1.486 ± 0.46

5.317 ± 0.345

5.16 ± 1.373

9.851 ± 1.647

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.049 ± 0.754

3.909 ± 0.342

5.317 ± 0.809

4.926 ± 1.06

8.21 ± 0.997

5.16 ± 0.749

4.378 ± 0.493

6.724 ± 0.845

1.486 ± 0.313

4.066 ± 0.798
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski