MSSI2.225 virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemykibivirus; Gemykibivirus humas1; Human associated gemykibivirus 1

Average proteome isoelectric point is 6.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A077XLY5|A0A077XLY5_9VIRU Replication associated protein OS=MSSI2.225 virus OX=1516081 GN=rep PE=4 SV=1
MM1 pKa = 7.68GKK3 pKa = 8.35TIWARR8 pKa = 11.84SLGNHH13 pKa = 7.12AYY15 pKa = 10.41FGGLFSLDD23 pKa = 3.56EE24 pKa = 4.33NLDD27 pKa = 3.61GVEE30 pKa = 3.93YY31 pKa = 10.64AIFDD35 pKa = 3.87DD36 pKa = 4.21FGGIKK41 pKa = 10.32FLPSYY46 pKa = 10.27KK47 pKa = 10.0FWLGHH52 pKa = 4.73QKK54 pKa = 9.95QFYY57 pKa = 8.86VTDD60 pKa = 3.67KK61 pKa = 11.21YY62 pKa = 10.94KK63 pKa = 10.96GKK65 pKa = 10.68KK66 pKa = 8.0LVHH69 pKa = 6.36WARR72 pKa = 11.84PSIWLSNSDD81 pKa = 3.6PRR83 pKa = 11.84DD84 pKa = 3.43EE85 pKa = 5.63LGVDD89 pKa = 4.0TEE91 pKa = 4.54WLNANCDD98 pKa = 3.81FVYY101 pKa = 10.57LDD103 pKa = 4.01SPIVSS108 pKa = 3.68

Molecular weight: 12.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A077XL96|A0A077XL96_9VIRU Replication protein OS=MSSI2.225 virus OX=1516081 GN=Rep PE=3 SV=1
MM1 pKa = 7.18SVARR5 pKa = 11.84RR6 pKa = 11.84SGRR9 pKa = 11.84RR10 pKa = 11.84TRR12 pKa = 11.84KK13 pKa = 8.7AFRR16 pKa = 11.84RR17 pKa = 11.84GGRR20 pKa = 11.84ATRR23 pKa = 11.84SRR25 pKa = 11.84GRR27 pKa = 11.84STRR30 pKa = 11.84RR31 pKa = 11.84SSRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84VRR38 pKa = 11.84RR39 pKa = 11.84RR40 pKa = 11.84TMTTRR45 pKa = 11.84RR46 pKa = 11.84VLNITTRR53 pKa = 11.84KK54 pKa = 9.91KK55 pKa = 9.8VDD57 pKa = 2.92NMLPVVVAEE66 pKa = 4.85DD67 pKa = 3.37NTTTVGPYY75 pKa = 9.82TSVSPLLSLFVPNARR90 pKa = 11.84TTRR93 pKa = 11.84TPVTNPAVRR102 pKa = 11.84NSSDD106 pKa = 2.71IFAVGYY112 pKa = 8.9RR113 pKa = 11.84EE114 pKa = 3.91RR115 pKa = 11.84VRR117 pKa = 11.84IDD119 pKa = 3.2LMGGGTFMWRR129 pKa = 11.84RR130 pKa = 11.84IVFMLKK136 pKa = 10.54GSDD139 pKa = 3.54LRR141 pKa = 11.84VAMNSSDD148 pKa = 3.6SGNIPNQLFDD158 pKa = 3.24QTTEE162 pKa = 4.13GGCRR166 pKa = 11.84RR167 pKa = 11.84VIGPLLGVTNARR179 pKa = 11.84DD180 pKa = 3.49EE181 pKa = 4.29LQSYY185 pKa = 7.08VFRR188 pKa = 11.84GQEE191 pKa = 3.7DD192 pKa = 4.19SDD194 pKa = 3.57WADD197 pKa = 3.23QFTAPLDD204 pKa = 3.63TRR206 pKa = 11.84RR207 pKa = 11.84ITVKK211 pKa = 10.22SDD213 pKa = 2.63KK214 pKa = 10.77LRR216 pKa = 11.84VIRR219 pKa = 11.84PGNEE223 pKa = 3.34TGASRR228 pKa = 11.84LFKK231 pKa = 9.76FWYY234 pKa = 8.43PIRR237 pKa = 11.84RR238 pKa = 11.84TISYY242 pKa = 10.58EE243 pKa = 3.91DD244 pKa = 4.05DD245 pKa = 3.97LEE247 pKa = 6.14SDD249 pKa = 4.19VVGDD253 pKa = 4.43RR254 pKa = 11.84PFSTAGLRR262 pKa = 11.84GVGDD266 pKa = 4.13MYY268 pKa = 11.73VMDD271 pKa = 4.6IMGITNLVNDD281 pKa = 5.3APPTSYY287 pKa = 10.97RR288 pKa = 11.84FGPEE292 pKa = 3.53GSFYY296 pKa = 9.46WHH298 pKa = 6.64EE299 pKa = 4.04RR300 pKa = 3.36

Molecular weight: 34.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

640

108

300

213.3

23.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.875 ± 1.799

1.25 ± 0.646

6.406 ± 0.885

4.375 ± 0.78

4.844 ± 0.671

9.219 ± 0.581

1.406 ± 0.666

3.906 ± 0.235

3.281 ± 1.091

7.5 ± 0.992

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.031 ± 0.796

4.219 ± 0.161

4.688 ± 0.276

2.656 ± 0.77

10.625 ± 3.054

7.187 ± 0.564

6.563 ± 1.732

7.656 ± 0.704

2.188 ± 0.705

3.125 ± 0.635
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski