Streptomyces phage Toma

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Arquatrovirinae; Likavirus; unclassified Likavirus

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 76 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U8UUB6|A0A2U8UUB6_9CAUD Uncharacterized protein OS=Streptomyces phage Toma OX=2182323 GN=69 PE=4 SV=1
MM1 pKa = 7.4IGGVVRR7 pKa = 11.84GSDD10 pKa = 3.78FRR12 pKa = 11.84CWTCVDD18 pKa = 4.41FSSYY22 pKa = 9.55EE23 pKa = 3.99RR24 pKa = 11.84EE25 pKa = 4.38TNSGDD30 pKa = 3.43AAEE33 pKa = 4.48KK34 pKa = 9.54CTYY37 pKa = 10.36CKK39 pKa = 10.73GPVWSQEE46 pKa = 3.84EE47 pKa = 4.33TTVNVDD53 pKa = 2.25IDD55 pKa = 4.27TVQEE59 pKa = 4.09YY60 pKa = 11.2ADD62 pKa = 3.75NALEE66 pKa = 4.36RR67 pKa = 11.84PSDD70 pKa = 3.32AAFWDD75 pKa = 3.94DD76 pKa = 3.64RR77 pKa = 11.84CYY79 pKa = 8.14TTHH82 pKa = 7.01APVIGWADD90 pKa = 3.62RR91 pKa = 11.84GDD93 pKa = 4.95DD94 pKa = 3.38ILEE97 pKa = 4.06EE98 pKa = 4.48SNYY101 pKa = 10.22HH102 pKa = 4.97SAKK105 pKa = 10.73ALIEE109 pKa = 4.29GAATEE114 pKa = 4.46DD115 pKa = 3.28EE116 pKa = 4.67HH117 pKa = 8.99VFEE120 pKa = 4.88GTAGHH125 pKa = 6.71WLVGSLSQVWVQVYY139 pKa = 7.0EE140 pKa = 4.13TRR142 pKa = 11.84PEE144 pKa = 4.09CSTIGCEE151 pKa = 4.46EE152 pKa = 3.96EE153 pKa = 4.18SEE155 pKa = 4.3YY156 pKa = 11.22LATYY160 pKa = 8.87TLASYY165 pKa = 10.76DD166 pKa = 3.71SEE168 pKa = 4.71GFCAEE173 pKa = 4.13HH174 pKa = 7.35KK175 pKa = 9.46EE176 pKa = 4.05ALEE179 pKa = 4.22GAFLAEE185 pKa = 4.15LLRR188 pKa = 11.84IEE190 pKa = 5.38FEE192 pKa = 3.85DD193 pKa = 3.72HH194 pKa = 6.89PRR196 pKa = 11.84DD197 pKa = 3.49FTAAFIEE204 pKa = 4.27AVEE207 pKa = 4.07IQEE210 pKa = 4.21ALKK213 pKa = 10.42DD214 pKa = 3.96YY215 pKa = 10.28PILDD219 pKa = 3.47EE220 pKa = 4.27TDD222 pKa = 3.43FSEE225 pKa = 5.25RR226 pKa = 11.84EE227 pKa = 3.77WEE229 pKa = 4.01RR230 pKa = 11.84FEE232 pKa = 5.12EE233 pKa = 4.14NSKK236 pKa = 10.6EE237 pKa = 4.19AIEE240 pKa = 4.2EE241 pKa = 4.11AQRR244 pKa = 11.84SHH246 pKa = 7.16PEE248 pKa = 3.79DD249 pKa = 4.05SIEE252 pKa = 5.6DD253 pKa = 3.19ITEE256 pKa = 3.58IEE258 pKa = 3.79NRR260 pKa = 11.84IFSEE264 pKa = 4.31SALSDD269 pKa = 3.4LFGYY273 pKa = 9.58EE274 pKa = 4.0GNAEE278 pKa = 4.19VSWDD282 pKa = 3.82AVADD286 pKa = 3.73IYY288 pKa = 11.6DD289 pKa = 4.23EE290 pKa = 4.37YY291 pKa = 11.39RR292 pKa = 11.84DD293 pKa = 3.69AYY295 pKa = 9.87FLEE298 pKa = 4.93RR299 pKa = 11.84ATEE302 pKa = 4.26VYY304 pKa = 9.2RR305 pKa = 11.84WNYY308 pKa = 10.13LGYY311 pKa = 10.9NPDD314 pKa = 3.4QLEE317 pKa = 4.25LFVIIIAAA325 pKa = 3.66

Molecular weight: 37.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2U8UU58|A0A2U8UU58_9CAUD Uncharacterized protein OS=Streptomyces phage Toma OX=2182323 GN=65 PE=4 SV=1
MM1 pKa = 7.5AGWSGSDD8 pKa = 2.78RR9 pKa = 11.84RR10 pKa = 11.84QRR12 pKa = 11.84LPQNWAQIRR21 pKa = 11.84RR22 pKa = 11.84RR23 pKa = 11.84ILRR26 pKa = 11.84RR27 pKa = 11.84DD28 pKa = 3.24GNRR31 pKa = 11.84CVWVFEE37 pKa = 4.3GKK39 pKa = 9.93RR40 pKa = 11.84CEE42 pKa = 4.2EE43 pKa = 4.1VATEE47 pKa = 4.03VDD49 pKa = 4.17HH50 pKa = 7.66IIAGDD55 pKa = 3.55NHH57 pKa = 7.38DD58 pKa = 4.65DD59 pKa = 3.68SNLRR63 pKa = 11.84SLCSWHH69 pKa = 6.31HH70 pKa = 4.87QKK72 pKa = 10.65KK73 pKa = 10.28SSSEE77 pKa = 3.48GRR79 pKa = 11.84AAQLAKK85 pKa = 10.19RR86 pKa = 11.84RR87 pKa = 11.84QIEE90 pKa = 3.73KK91 pKa = 9.69RR92 pKa = 11.84FRR94 pKa = 11.84RR95 pKa = 11.84NEE97 pKa = 3.86SHH99 pKa = 7.53PGLLL103 pKa = 4.09

Molecular weight: 12.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

76

0

76

15820

39

1160

208.2

22.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.335 ± 0.376

0.721 ± 0.105

6.403 ± 0.228

7.143 ± 0.468

3.198 ± 0.154

8.666 ± 0.431

2.042 ± 0.184

4.576 ± 0.37

4.469 ± 0.225

8.407 ± 0.418

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.212 ± 0.162

3.104 ± 0.151

4.829 ± 0.19

3.274 ± 0.159

6.391 ± 0.398

5.885 ± 0.275

6.391 ± 0.342

7.149 ± 0.252

1.934 ± 0.121

2.87 ± 0.276
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski