Microbacterium sp. dk485

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Microbacterium; unclassified Microbacterium

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3585 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y9PK63|A0A4Y9PK63_9MICO LysE family translocator OS=Microbacterium sp. dk485 OX=2560021 GN=E4V99_17155 PE=4 SV=1
MM1 pKa = 7.46SGAALAVTALLVAGCSAGEE20 pKa = 4.12GVDD23 pKa = 4.3LDD25 pKa = 3.85SSGADD30 pKa = 3.32GEE32 pKa = 4.73VLVAAIGGEE41 pKa = 4.05PDD43 pKa = 3.24QLDD46 pKa = 3.79PQKK49 pKa = 8.03TTSYY53 pKa = 10.51FAFEE57 pKa = 3.92VLEE60 pKa = 4.21NVFDD64 pKa = 4.06TLVEE68 pKa = 4.24PDD70 pKa = 4.9EE71 pKa = 4.24NLQMQPALAEE81 pKa = 4.09SWEE84 pKa = 4.14VSDD87 pKa = 6.39DD88 pKa = 3.6QLEE91 pKa = 4.38WTFTLRR97 pKa = 11.84DD98 pKa = 3.35GVTFHH103 pKa = 7.53DD104 pKa = 4.55GSEE107 pKa = 4.21FTSADD112 pKa = 2.79VMYY115 pKa = 10.28SYY117 pKa = 11.53DD118 pKa = 4.08RR119 pKa = 11.84IIDD122 pKa = 3.92EE123 pKa = 4.56EE124 pKa = 4.85LSNAWKK130 pKa = 9.4FASVTDD136 pKa = 3.42VTAPDD141 pKa = 3.91DD142 pKa = 3.66QTVVITVAQPTPNLLSNLGGFKK164 pKa = 10.63GMAIVQEE171 pKa = 4.51DD172 pKa = 3.87NVASGDD178 pKa = 3.32ITTAPVGTGPFSVEE192 pKa = 4.01EE193 pKa = 4.33YY194 pKa = 10.93VSGDD198 pKa = 4.04HH199 pKa = 6.45ITLTANPDD207 pKa = 3.26YY208 pKa = 10.71WGGAPEE214 pKa = 4.45LGGVEE219 pKa = 3.94YY220 pKa = 10.86RR221 pKa = 11.84FISEE225 pKa = 4.15PATALAALRR234 pKa = 11.84GGEE237 pKa = 4.14IQWTDD242 pKa = 3.26VVPPQQVGEE251 pKa = 4.4LEE253 pKa = 3.92TDD255 pKa = 3.18EE256 pKa = 5.15ALEE259 pKa = 4.46LGVTPSSDD267 pKa = 3.04YY268 pKa = 10.62WYY270 pKa = 10.62LALNEE275 pKa = 4.21ARR277 pKa = 11.84EE278 pKa = 4.18PWSDD282 pKa = 3.19VRR284 pKa = 11.84VRR286 pKa = 11.84QAIAYY291 pKa = 9.59AIDD294 pKa = 3.59RR295 pKa = 11.84DD296 pKa = 4.36AIVQAVSYY304 pKa = 8.07GTAEE308 pKa = 4.2LNQLAIPEE316 pKa = 4.01QSVWYY321 pKa = 8.39TPYY324 pKa = 9.85DD325 pKa = 3.68TYY327 pKa = 11.13STDD330 pKa = 3.15MDD332 pKa = 4.07TAQQLMAEE340 pKa = 4.34AGFTGGTLDD349 pKa = 5.27LLATSDD355 pKa = 3.86YY356 pKa = 10.71PEE358 pKa = 4.57TVTAAQIIAANLEE371 pKa = 3.98PLGIEE376 pKa = 4.22VAIRR380 pKa = 11.84QPDD383 pKa = 4.05FSTWLDD389 pKa = 3.61EE390 pKa = 4.3QNSGNFDD397 pKa = 3.71MLMMGWLGNIDD408 pKa = 4.23PDD410 pKa = 3.72DD411 pKa = 4.38FYY413 pKa = 11.75YY414 pKa = 10.64SQHH417 pKa = 6.51HH418 pKa = 5.6STGASNAQKK427 pKa = 10.3YY428 pKa = 9.85ANPEE432 pKa = 3.45VDD434 pKa = 3.3QLLDD438 pKa = 3.52AGRR441 pKa = 11.84VEE443 pKa = 4.56TDD445 pKa = 2.89TEE447 pKa = 4.08ARR449 pKa = 11.84KK450 pKa = 9.99DD451 pKa = 3.77LYY453 pKa = 11.11AQAATIIADD462 pKa = 3.73EE463 pKa = 4.11ASYY466 pKa = 10.53IYY468 pKa = 10.34LYY470 pKa = 10.51NPSVIQAWSPSITGYY485 pKa = 8.02EE486 pKa = 3.73ARR488 pKa = 11.84ADD490 pKa = 3.79RR491 pKa = 11.84AIRR494 pKa = 11.84FRR496 pKa = 11.84DD497 pKa = 3.38AALSGG502 pKa = 3.56

Molecular weight: 54.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y9PXU4|A0A4Y9PXU4_9MICO Phenylacetate-CoA oxygenase subunit PaaJ OS=Microbacterium sp. dk485 OX=2560021 GN=paaJ PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3585

0

3585

1157956

29

2480

323.0

34.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.219 ± 0.066

0.49 ± 0.009

6.223 ± 0.037

5.615 ± 0.038

3.072 ± 0.026

9.034 ± 0.035

1.976 ± 0.022

4.237 ± 0.032

1.541 ± 0.03

10.165 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.765 ± 0.015

1.729 ± 0.02

5.7 ± 0.031

2.65 ± 0.019

7.759 ± 0.048

5.293 ± 0.026

5.852 ± 0.032

9.16 ± 0.035

1.564 ± 0.019

1.955 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski